BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,273,758	A→T	5.2%	G228G (GGA→GGT)	mtlA →	PTS system mannitol‑specific EIICB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,273,758	0	A	T	5.2%	394.4 / 7.4	114	G228G (GGA→GGT)	mtlA	PTS system mannitol‑specific EIICB component
Reads supporting (aligned to +/- strand): ref base A (55/53); new base T (4/2); total (59/55)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 116 total aligned reads displayed.

GATTAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTA  >  USA300TCH1516_ALE/2273668‑2273848

gATTAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGa                                                                                            <  1:2586097/91‑1 (MQ=255)

  ttAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGAcc                                                                                          >  1:595875/1‑91 (MQ=255)

  ttAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGAcc                                                                                          <  1:339273/91‑1 (MQ=255)

      ccATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGt                                                                                      >  1:2994502/1‑91 (MQ=255)

      ccATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGt                                                                                      >  1:2996704/1‑91 (MQ=255)

      ccATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGt                                                                                      >  1:2995343/1‑91 (MQ=255)

         tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTa                                                                                   >  1:768146/1‑91 (MQ=255)

         tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTa                                                                                   <  1:2294304/91‑1 (MQ=255)

         tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTa                                                                                   <  1:3961042/91‑1 (MQ=255)

           gtgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAgg                                                                                 >  1:1923479/1‑91 (MQ=255)

           gtgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAgg                                                                                 <  1:4080754/91‑1 (MQ=255)

            tgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGt                                                                                <  1:2196140/91‑1 (MQ=255)

            tgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGt                                                                                <  1:3801461/91‑1 (MQ=255)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCAGAAGCTGGTCAATCAATTTTATACACAATTGAATCTAAACCTGGTCCAGGGTTAgatt                                                                               <  1:3398853/91‑4 (MQ=38)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCACAAGCTGGTCAATCAATTTTATACACAATTGAATCTAAACCTGGTCCAGGGTTAgatt                                                                               <  1:3398739/91‑4 (MQ=38)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGATCAGGTTTAGGTa                                                                               <  1:3183184/91‑1 (MQ=255)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                               >  1:817573/1‑91 (MQ=255)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                               <  1:1428736/91‑1 (MQ=255)

             gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                               <  1:1428469/91‑1 (MQ=255)

              tATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTAt                                                                              >  1:2122091/1‑91 (MQ=255)

              tATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTAt                                                                              >  1:3906647/1‑91 (MQ=255)

               aTTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATc                                                                             <  1:2317176/91‑1 (MQ=255)

                  cacaCCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTa                                                                          <  1:2053009/91‑1 (MQ=255)

                   acacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTAc                                                                         <  1:3563414/91‑1 (MQ=255)

                    cacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACt                                                                        >  1:1240084/1‑91 (MQ=255)

                    cacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACt                                                                        <  1:2385967/91‑1 (MQ=255)

                     acCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACtt                                                                       >  1:3787984/1‑91 (MQ=255)

                     acCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACtt                                                                       >  1:3055109/1‑91 (MQ=255)

                     acCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACtt                                                                       >  1:3055139/1‑91 (MQ=255)

                       cGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGc                                                                     >  1:1405031/1‑91 (MQ=255)

                       cGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGc                                                                     >  1:651583/1‑91 (MQ=255)

                        gCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCt                                                                    <  1:75747/91‑1 (MQ=255)

                           tGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTtat                                                                 <  1:439310/91‑1 (MQ=255)

                             gTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTatat                                                               >  1:2636858/1‑91 (MQ=255)

                             gTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTatat                                                               >  1:2639909/1‑91 (MQ=255)

                               gCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGa                                                             >  1:669225/1‑91 (MQ=255)

                               gCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGa                                                             >  1:669738/1‑91 (MQ=255)

                                 aGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGAtt                                                           <  1:3614209/91‑1 (MQ=255)

                                 aGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCATATATGAtt                                                           <  1:2089750/91‑1 (MQ=255)

                                     cAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTg                                                       <  1:524594/91‑1 (MQ=255)

                                      aaGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTgg                                                      <  1:3607346/91‑1 (MQ=255)

                                      aaGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTgg                                                      <  1:3607857/91‑1 (MQ=255)

                                        gCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGaa                                                    >  1:930059/1‑91 (MQ=255)

                                            cAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAgg                                                >  1:4137874/1‑91 (MQ=255)

                                            cAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAgg                                                <  1:2364114/91‑1 (MQ=255)

                                            cAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGCTATCTTACTTGCTTATATGATTTTTGGAAAAgg                                                >  1:3395753/1‑91 (MQ=255)

                                             aaaaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGc                                               <  1:1732177/91‑1 (MQ=255)

                                             aaaaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGc                                               <  1:3516298/91‑1 (MQ=255)

                                             aaaaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGc                                               <  1:3516241/91‑1 (MQ=255)

                                               aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                             >  1:4075832/1‑91 (MQ=255)

                                               aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                             <  1:1213259/91‑1 (MQ=255)

                                               aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                             <  1:1212908/91‑1 (MQ=255)

                                                aGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACt                                            >  1:1140555/1‑91 (MQ=255)

                                                aGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACt                                            >  1:2187213/1‑91 (MQ=255)

                                                aGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACt                                            >  1:3256675/1‑91 (MQ=255)

                                                 gCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTg                                           <  1:3805139/91‑1 (MQ=255)

                                                 gCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTg                                           <  1:3878690/91‑1 (MQ=255)

                                                   tGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCa                                         >  1:2879743/1‑91 (MQ=255)

                                                   tGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACAGCa                                         >  1:3213788/1‑91 (MQ=255)

                                                    ggtgaatcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAGAAGGCACTGCaa                                        <  1:3209520/87‑1 (MQ=255)

                                                    ggTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCaa                                        >  1:2508107/1‑91 (MQ=255)

                                                    ggTCAATCAATTTTATACACAATTGAATCTAACCCTGCACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCaa                                        >  1:3497060/1‑91 (MQ=255)

                                                          tcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCaa                                  <  1:3439153/91‑1 (MQ=255)

                                                             atTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGt                               <  1:1612902/91‑1 (MQ=255)

                                                              ttttATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTc                              >  1:1639794/1‑91 (MQ=255)

                                                               tttATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCa                             <  1:4148284/91‑1 (MQ=255)

                                                                 tataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCata                           <  1:1172824/91‑1 (MQ=255)

                                                                 tataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCata                           >  1:3643911/1‑91 (MQ=255)

                                                                  ataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCatat                          <  1:1460977/91‑1 (MQ=255)

                                                                  ataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCatat                          >  1:2542890/1‑91 (MQ=255)

                                                                  ataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCatat                          >  1:3099299/1‑91 (MQ=255)

                                                                   taCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATg                         <  1:3697012/91‑1 (MQ=255)

                                                                    acacAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATgg                        >  1:1218234/1‑91 (MQ=255)

                                                                    acacAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATgg                        >  1:1218448/1‑91 (MQ=255)

                                                                     cacaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGt                       <  1:2847179/91‑1 (MQ=255)

                                                                     cacaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGt                       <  1:3434804/91‑1 (MQ=255)

                                                                     cacaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACATGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGt                       >  1:3598522/1‑91 (MQ=255)

                                                                      acaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTg                      >  1:1700715/1‑91 (MQ=255)

                                                                       caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                     <  1:135080/91‑1 (MQ=255)

                                                                       caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                     <  1:3868672/91‑1 (MQ=255)

                                                                       caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                     <  1:2060194/91‑1 (MQ=255)

                                                                       caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                     >  1:2988716/1‑91 (MQ=255)

                                                                       caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                     >  1:3383428/1‑91 (MQ=255)

                                                                        aaTTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCt                    <  1:2815738/91‑1 (MQ=255)

                                                                         aTTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTg                   >  1:2191665/1‑91 (MQ=255)

                                                                          ttGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTgg                  <  1:298862/91‑1 (MQ=255)

                                                                            ataccTAAACCTGGTCCAGGGTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGaa                >  1:2555586/5‑91 (MQ=38)

                                                                              aTCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAAtt              >  1:4056966/1‑91 (MQ=255)

                                                                              aTCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAAtt              >  1:3607282/1‑91 (MQ=255)

                                                                               ccTAAACCTGGTCCAGGGTTAGATATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTa             >  1:1599594/2‑91 (MQ=38)

                                                                                 taaaccTGGTCCAGGGTTAGGTATCTTACTTGCTTATATGATTTTTGG‑AAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATcc          >  1:2013900/5‑91 (MQ=255)

                                                                                 tAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATc           >  1:634707/1‑91 (MQ=255)

                                                                                 tAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATc           >  1:1348423/1‑91 (MQ=255)

                                                                                 tAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATc           >  1:798697/1‑91 (MQ=255)

                                                                                  aaCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATcc          <  1:3006556/91‑1 (MQ=255)

                                                                                  aaCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATcc          <  1:867283/91‑1 (MQ=255)

                                                                                   aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa         >  1:1686834/1‑91 (MQ=255)

                                                                                   aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa         >  1:1829549/1‑91 (MQ=255)

                                                                                   aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa         >  1:2008913/1‑91 (MQ=255)

                                                                                          aCCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTa  >  1:2883190/1‑91 (MQ=255)

GATTAACCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTA  >  USA300TCH1516_ALE/2273668‑2273848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`