BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,273,764	T→G	6.0%	G230G (GGT→GGG)	mtlA →	PTS system mannitol‑specific EIICB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,273,764	0	T	G	6.0%	387.9 / 14.6	117	G230G (GGT→GGG)	mtlA	PTS system mannitol‑specific EIICB component
Reads supporting (aligned to +/- strand): ref base T (54/56); new base G (5/2); total (59/58)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

Only 100 of 121 total aligned reads displayed.

CCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGG  >  USA300TCH1516_ALE/2273674‑2273853

ccATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGt                                                                                           >  1:2994502/1‑91 (MQ=255)

ccATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGt                                                                                           >  1:2996704/1‑91 (MQ=255)

   tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGTCCAGGTTTa                                                                                        >  1:3724299/1‑91 (MQ=255)

   tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTa                                                                                        <  1:3961042/91‑1 (MQ=255)

   tGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTa                                                                                        <  1:2294304/91‑1 (MQ=255)

     gtgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAgg                                                                                      >  1:1923479/1‑91 (MQ=255)

      tgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGt                                                                                     <  1:3801461/91‑1 (MQ=255)

      tgtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGt                                                                                     <  1:2196140/91‑1 (MQ=255)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCAGAAGCTGGTCAATCAATTTTATACACAATTGAATCTAAACCTGGTCCAGGGTTAgatt                                                                                    <  1:3398853/91‑4 (MQ=38)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCACAAGCTGGTCAATCAATTTTATACACAATTGAATCTAAACCTGGTCCAGGGTTAgatt                                                                                    <  1:3398739/91‑4 (MQ=38)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGATCAGGTTTAGGTa                                                                                    <  1:3183184/91‑1 (MQ=255)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                                    <  1:1428736/91‑1 (MQ=255)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                                    <  1:1428469/91‑1 (MQ=255)

       gtATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTa                                                                                    >  1:817573/1‑91 (MQ=255)

        tATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTAt                                                                                   >  1:2122091/1‑91 (MQ=255)

        tATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTAt                                                                                   >  1:3906647/1‑91 (MQ=255)

         aTTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATc                                                                                  <  1:2317176/91‑1 (MQ=255)

            cacaCCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTa                                                                               <  1:232111/91‑1 (MQ=255)

            cacaCCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTa                                                                               <  1:2053009/91‑1 (MQ=255)

             acacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTAc                                                                              <  1:3563414/91‑1 (MQ=255)

              cacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACt                                                                             <  1:2385967/91‑1 (MQ=255)

              cacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACt                                                                             >  1:1553322/1‑91 (MQ=255)

              cacCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACt                                                                             >  1:1240084/1‑91 (MQ=255)

               acCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACtt                                                                            >  1:3055139/1‑91 (MQ=255)

               acCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACtt                                                                            >  1:3055109/1‑91 (MQ=255)

                 cGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGc                                                                          >  1:651583/1‑91 (MQ=255)

                  gCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCt                                                                         <  1:75747/91‑1 (MQ=255)

                    ttGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTta                                                                       <  1:669952/91‑1 (MQ=255)

                     tGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTtat                                                                      <  1:439310/91‑1 (MQ=255)

                      ggTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTtata                                                                     >  1:2602709/1‑91 (MQ=255)

                       gTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTatat                                                                    >  1:2073098/1‑91 (MQ=255)

                       gTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTatat                                                                    >  1:2639909/1‑91 (MQ=255)

                         gCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGa                                                                  >  1:669225/1‑91 (MQ=255)

                         gCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGa                                                                  >  1:669738/1‑91 (MQ=255)

                           aGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGAtt                                                                <  1:3614209/91‑1 (MQ=255)

                           aGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCATATATGAtt                                                                <  1:2089750/91‑1 (MQ=255)

                             aTCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGAtttt                                                              <  1:1335329/91‑1 (MQ=255)

                               cAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTg                                                            <  1:524594/91‑1 (MQ=255)

                                aaGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTgg                                                           <  1:3607857/91‑1 (MQ=255)

                                  gCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGaa                                                         >  1:930059/1‑91 (MQ=255)

                                      cAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAgg                                                     >  1:4137874/1‑91 (MQ=255)

                                      cAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGCTATCTTACTTGCTTATATGATTTTTGGAAAAgg                                                     >  1:3395753/1‑91 (MQ=255)

                                       aaaaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGc                                                    <  1:3516298/91‑1 (MQ=255)

                                         aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                                  <  1:1212908/91‑1 (MQ=255)

                                         aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                                  <  1:1213259/91‑1 (MQ=255)

                                         aaGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCAc                                                  >  1:4075832/1‑91 (MQ=255)

                                          aGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACt                                                 >  1:1140555/1‑91 (MQ=255)

                                          aGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACt                                                 >  1:3256675/1‑91 (MQ=255)

                                           gCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTg                                                <  1:3878690/91‑1 (MQ=255)

                                           gCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTg                                                <  1:3805139/91‑1 (MQ=255)

                                            cTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGc                                               <  1:509006/91‑1 (MQ=255)

                                             tGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAACGGCACTGCa                                              >  1:3979727/1‑91 (MQ=255)

                                             tGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACAGCa                                              >  1:3213788/1‑91 (MQ=255)

                                              ggtgaatcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAGAAGGCACTGCaa                                             <  1:3209520/87‑1 (MQ=255)

                                              ggTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCaa                                             >  1:2508107/1‑91 (MQ=255)

                                              ggTCAATCAATTTTATACACAATTGAATCTAACCCTGCACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCaa                                             >  1:3497060/1‑91 (MQ=255)

                                                    tcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCaa                                       <  1:3439124/91‑1 (MQ=255)

                                                    tcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCaa                                       <  1:3439153/91‑1 (MQ=255)

                                                    tcaatTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCaa                                       <  1:3035296/91‑1 (MQ=255)

                                                       atTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGt                                    <  1:1612902/91‑1 (MQ=255)

                                                        ttttATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTc                                   >  1:1639794/1‑91 (MQ=255)

                                                         tttATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCa                                  <  1:4148284/91‑1 (MQ=255)

                                                           tataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCata                                <  1:1172824/91‑1 (MQ=255)

                                                           tataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCata                                >  1:3643911/1‑91 (MQ=255)

                                                            ataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCatat                               <  1:1460977/91‑1 (MQ=255)

                                                            ataCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCatat                               >  1:2542890/1‑91 (MQ=255)

                                                             taCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATg                              >  1:2954465/1‑91 (MQ=255)

                                                             taCACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATg                              <  1:3697012/91‑1 (MQ=255)

                                                              acacAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATgg                             >  1:1218448/1‑91 (MQ=255)

                                                               cacaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGt                            <  1:2847179/91‑1 (MQ=255)

                                                               cacaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACATGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGt                            >  1:3598522/1‑91 (MQ=255)

                                                                acaATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTg                           >  1:1700715/1‑91 (MQ=255)

                                                                 caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                          <  1:3868672/91‑1 (MQ=255)

                                                                 caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                          >  1:2988716/1‑91 (MQ=255)

                                                                 caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                          >  1:3383428/1‑91 (MQ=255)

                                                                 caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                          <  1:2060194/91‑1 (MQ=255)

                                                                 caATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGc                          <  1:135080/91‑1 (MQ=255)

                                                                  aaTTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCt                         <  1:2815738/91‑1 (MQ=255)

                                                                   aTTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTg                        >  1:2191665/1‑91 (MQ=255)

                                                                    ttGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTgg                       <  1:1782055/91‑1 (MQ=255)

                                                                    ttGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTgg                       <  1:298862/91‑1 (MQ=255)

                                                                      ataccTAAACCTGGTCCAGGGTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGaa                     >  1:2555586/5‑91 (MQ=38)

                                                                        aTCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAAtt                   >  1:4056966/1‑91 (MQ=255)

                                                                        aTCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAAtt                   >  1:3607282/1‑91 (MQ=255)

                                                                         tCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTa                  >  1:3603002/1‑91 (MQ=255)

                                                                           tAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATc                >  1:798697/1‑91 (MQ=255)

                                                                           tAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATc                >  1:1348423/1‑91 (MQ=255)

                                                                            aaCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATcc               <  1:867283/91‑1 (MQ=255)

                                                                            aaCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATcc               <  1:3006556/91‑1 (MQ=255)

                                                                             aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa              >  1:1829549/1‑91 (MQ=255)

                                                                             aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa              >  1:1686834/1‑91 (MQ=255)

                                                                             aCCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCa              >  1:2008913/1‑91 (MQ=255)

                                                                                 tggtccAGGGTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCActtc          >  1:2689550/5‑91 (MQ=255)

                                                                                 tggtccAGGGTTAGGTATCTTACTTGCTTATATGATTCTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCAcctc          >  1:3501641/5‑91 (MQ=255)

                                                                                    aCCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTa       >  1:2883190/1‑91 (MQ=255)

                                                                                      cAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAgg     <  1:1155036/91‑1 (MQ=255)

                                                                                       aggtataggtatCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAggt    <  1:3460556/91‑1 (MQ=255)

                                                                                       aGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAggt    <  1:144460/91‑1 (MQ=255)

                                                                                       aGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAggt    >  1:1691164/1‑91 (MQ=255)

                                                                                         gTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAggtgg  <  1:3253977/91‑1 (MQ=255)

CCATGGTGTATTCACACCGCTTGGTGCAGATCAAGCTGCAAAAGCTGGTCAATCAATTTTATACACAATTGAATCTAACCCTGGACCAGGTTTAGGTATCTTACTTGCTTATATGATTTTTGGAAAAGGCACTGCAAAAGCAACGTCATATGGTGCTGGAATTATCCACTTCTTAGGTGG  >  USA300TCH1516_ALE/2273674‑2273853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`