BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,275,551	A→G	7.8%	T302A (ACA→GCA) ‡	mtlR →	Transcriptional regulator MtlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,275,551	0	A	G	7.8%	401.8 / 18.5	128	T302A (ACA→GCA) ‡	mtlR	Transcriptional regulator MtlR
Reads supporting (aligned to +/- strand): ref base A (67/51); new base G (4/6); total (71/57)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 131 total aligned reads displayed.

GGTCAAATATATGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAGATTTGG  >  USA300TCH1516_ALE/2275461‑2275641

ggTCAAATATATGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATa                                                                                            <  1:1943357/91‑1 (MQ=255)

 gTCAAATATATGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATAc                                                                                           <  1:724109/91‑1 (MQ=255)

 gTCAAATATATGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATAc                                                                                           >  1:2717372/1‑91 (MQ=255)

       tatatGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATGTATCAtt                                                                                     <  1:1353363/91‑1 (MQ=255)

          atGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATGTATCATTaag                                                                                  <  1:2913959/91‑2 (MQ=255)

          atGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATGTATCATTaag                                                                                  <  1:3645197/91‑2 (MQ=255)

          atGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATGTATCATTaag                                                                                  <  1:125784/91‑2 (MQ=255)

          atGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTaaa                                                                                  <  1:2939136/91‑1 (MQ=255)

          atGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTaaa                                                                                  >  1:3041891/1‑91 (MQ=255)

          atGACGTCACGTCTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTGCGTGGAGCTAAACGAAAAAATCTTAATGATACATCATaaaa                                                                                  >  1:3208093/1‑91 (MQ=255)

           tGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaat                                                                                 <  1:3801082/91‑1 (MQ=255)

            gACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaata                                                                                <  1:795600/91‑1 (MQ=255)

            gACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaata                                                                                <  1:3221753/91‑1 (MQ=255)

             aCGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaataa                                                                               >  1:3614527/1‑91 (MQ=255)

             aCGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaataa                                                                               >  1:3614952/1‑91 (MQ=255)

             aCGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAaataa                                                                               >  1:569578/1‑91 (MQ=255)

                tCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCg                                                                            <  1:1872404/91‑1 (MQ=255)

                   cGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtg                                                                         <  1:381175/91‑1 (MQ=255)

                   cGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtg                                                                         <  1:2730314/91‑1 (MQ=255)

                   cGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtg                                                                         <  1:2730270/91‑1 (MQ=255)

                    gTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtgt                                                                        >  1:3301808/1‑91 (MQ=255)

                    gTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtgt                                                                        <  1:1566533/91‑1 (MQ=255)

                    gTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtgt                                                                        >  1:2399371/1‑91 (MQ=255)

                     tttAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTtgtg                                                                       >  1:3358138/1‑91 (MQ=255)

                          tCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTgaagaa                                                                  <  1:3422057/91‑1 (MQ=255)

                          tCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTgaagaa                                                                  >  1:3481307/1‑91 (MQ=255)

                          tCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTgaagaa                                                                  <  1:900407/91‑1 (MQ=255)

                             ggCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAAc                                                               <  1:725620/91‑1 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              <  1:1007272/91‑1 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              >  1:2530719/1‑91 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              >  1:3571014/1‑91 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              >  1:2530551/1‑91 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              <  1:1216148/91‑1 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              >  1:2530203/1‑91 (MQ=255)

                              gCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACa                                                              <  1:238066/91‑1 (MQ=255)

                                   aaTTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAAtt                                                         >  1:1480194/1‑91 (MQ=255)

                                   aaTTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAAtt                                                         <  1:1147907/91‑1 (MQ=255)

                                     ttGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTaa                                                       >  1:1766114/1‑91 (MQ=255)

                                     ttGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTaa                                                       >  1:1389304/1‑91 (MQ=255)

                                      tGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTaaa                                                      <  1:2180748/91‑1 (MQ=255)

                                      tGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTaaa                                                      <  1:1140789/91‑1 (MQ=255)

                                       gCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAg                                                     >  1:3092695/1‑91 (MQ=255)

                                       gCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAg                                                     >  1:3092434/1‑91 (MQ=255)

                                       gCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAg                                                     >  1:1609270/1‑91 (MQ=255)

                                       gCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAg                                                     >  1:1567298/1‑91 (MQ=255)

                                       gCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAg                                                     >  1:2888546/1‑91 (MQ=255)

                                        cTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGc                                                    >  1:2254493/1‑91 (MQ=255)

                                           tCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGtt                                                 >  1:3452855/1‑91 (MQ=255)

                                               tACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGtt                                             <  1:3063350/91‑1 (MQ=255)

                                                  tATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAAc                                          >  1:542130/1‑91 (MQ=255)

                                                   aTCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACa                                         <  1:425238/91‑1 (MQ=255)

                                                    tCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACaa                                        >  1:3985069/1‑91 (MQ=255)

                                                    tCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACaa                                        >  1:3984917/1‑91 (MQ=255)

                                                     ccATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACaaa                                       >  1:2645953/1‑91 (MQ=255)

                                                      cATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAg                                      >  1:1614975/1‑91 (MQ=255)

                                                       aTTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGt                                     >  1:3957565/1‑91 (MQ=255)

                                                            cGTGGAGCTAAACGAAAGAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTACAGCGTTTGTTAACAAAGTAGaaa                                <  1:3557711/91‑1 (MQ=255)

                                                            cGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGaaa                                <  1:3733584/91‑1 (MQ=255)

                                                            cGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGaaa                                <  1:4134385/91‑1 (MQ=255)

                                                            cGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGaaa                                >  1:2748666/1‑91 (MQ=255)

                                                            cGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGaaa                                >  1:2329859/1‑91 (MQ=255)

                                                             gTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAAt                               <  1:584898/91‑1 (MQ=255)

                                                             gTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAAt                               <  1:1164725/91‑1 (MQ=255)

                                                             gTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAAt                               >  1:3812972/1‑91 (MQ=255)

                                                              tGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATg                              <  1:2166093/91‑1 (MQ=255)

                                                              tGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATg                              <  1:2165086/91‑1 (MQ=255)

                                                                gAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGAt                            >  1:1105552/1‑91 (MQ=255)

                                                                  gCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGAttt                          >  1:67551/1‑91 (MQ=255)

                                                                   cTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTc                         >  1:2886372/1‑91 (MQ=255)

                                                                    tAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTcc                        >  1:3389180/1‑91 (MQ=255)

                                                                    tAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTcc                        >  1:3040102/1‑91 (MQ=255)

                                                                     aaaCGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCg                       <  1:1066944/91‑1 (MQ=255)

                                                                     aaaCGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCg                       >  1:3126235/1‑91 (MQ=255)

                                                                      aaCGAAAAAATCTTAGTGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAACGCGTTTGTTAACAAAGTAGAAATGATTTCCgg                      >  1:3540208/1‑91 (MQ=255)

                                                                        cGAAAAAATCTTGATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTa                    >  1:2033069/1‑91 (MQ=255)

                                                                        cGAAAAAATCTTGATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTa                    >  1:2033040/1‑91 (MQ=255)

                                                                         gAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTAt                   <  1:2780118/91‑1 (MQ=255)

                                                                         gAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTAt                   >  1:1013401/1‑91 (MQ=255)

                                                                          aaaaaaTCTTAATGATACATCATTAAATAANCGTTGTNAAGAAAANAAAATTAAAGCNTTTGNTAACAAAGTAGAAATGATTTCCGGTATg                  >  1:1202074/1‑91 (MQ=255)

                                                                            aaaaTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATggc                <  1:1864935/91‑3 (MQ=255)

                                                                            aaaaTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGAc                >  1:3228181/1‑91 (MQ=255)

                                                                            aaaaTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGAc                >  1:3904755/1‑91 (MQ=255)

                                                                              aaTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAt              <  1:1026902/91‑1 (MQ=255)

                                                                              aaTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAt              <  1:1326866/91‑1 (MQ=255)

                                                                               tatttAATGATGTATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAtt             >  1:3008253/4‑91 (MQ=255)

                                                                               tatttAATGATGTATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAtt             >  1:3008336/4‑91 (MQ=255)

                                                                               aTCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAtt             <  1:121399/91‑1 (MQ=255)

                                                                                tCTTAATGATACCTCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAttt            >  1:3208954/1‑91 (MQ=255)

                                                                                tCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACAttt            <  1:3838557/91‑1 (MQ=255)

                                                                                 cTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTg           <  1:1002257/91‑1 (MQ=255)

                                                                                 cTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTg           >  1:3795964/1‑91 (MQ=255)

                                                                                 cTTAATGATACACCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTg           >  1:3205768/1‑91 (MQ=255)

                                                                                  ttAATGATGTATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGc          >  1:1316615/1‑91 (MQ=255)

                                                                                  ttAATGATGTATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGc          >  1:143627/1‑91 (MQ=255)

                                                                                  ttAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAACTTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGc          >  1:3584848/1‑91 (MQ=255)

                                                                                  ttAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGc          <  1:1138186/91‑1 (MQ=255)

                                                                                    aaTGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAg        >  1:1883453/1‑91 (MQ=255)

                                                                                       gATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAGAtt     <  1:468639/91‑1 (MQ=255)

                                                                                       gATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAGAtt     <  1:1564489/91‑1 (MQ=255)

                                                                                          aCATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAGATTTgg  >  1:779231/1‑91 (MQ=255)

GGTCAAATATATGACGTCACGTTTAATCAGGCAGAAATTGCTTTCATTACTATCCATTTACGTGGAGCTAAACGAAAAAATCTTAATGATACATCATTAAATAATCGTTGTGAAGAAAACAAAATTAAAGCGTTTGTTAACAAAGTAGAAATGATTTCCGGTATGACATTTGCAGATTTGG  >  USA300TCH1516_ALE/2275461‑2275641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`