BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,429,717	T→A	13.5%	intergenic (‑71/+62)	lytR_2 ← / ← suhB_2	Transcriptional regulator LytR/Inositol‑1‑monophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,429,717	0	T	A	13.5%	308.9 / 35.3	104	intergenic (‑71/+62)	lytR_2/suhB_2	Transcriptional regulator LytR/Inositol‑1‑monophosphatase
Reads supporting (aligned to +/- strand): ref base T (51/39); new base A (10/4); total (61/43)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 113 total aligned reads displayed.

CTTTTAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCATTTG  >  USA300TCH1516_ALE/2429627‑2429807

cTTTTAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAAttt                                                                                            >  1:3907043/1‑91 (MQ=255)

cTTTTAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAAttt                                                                                            >  1:1825608/1‑91 (MQ=255)

 ttttAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAAtttt                                                                                           <  1:2105069/91‑1 (MQ=255)

   ttAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAAtttttt                                                                                         >  1:3093780/1‑91 (MQ=255)

        cGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAAAAATTATTTTACta                                                                                    <  1:820443/91‑1 (MQ=255)

         gAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACtat                                                                                   <  1:1613418/91‑1 (MQ=255)

         gAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAAAAATTATTTTACtat                                                                                   <  1:176889/91‑1 (MQ=255)

          accGTTTCAATCCCCCCACTCCTTTCCTATTCCTTACCTACTTTGTATGTTTTCTCTATTTCCTATATAGTAAAAAACTTATTTTACtata                                                                                  <  1:2698035/89‑1 (MQ=255)

          aaCGTTTCAATCCCACCACTCATTTACTATTCCTTTCCTACTTTGTCTGTTTTCTCTATTTTTTATATAGTAAAATAATTTTTTTACtttt                                                                                  >  1:2736199/1‑88 (MQ=255)

              tttCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTc                                                                              >  1:271809/1‑91 (MQ=255)

              tttCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTc                                                                              <  1:140421/91‑1 (MQ=255)

                tCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTg                                                                            <  1:3872821/91‑1 (MQ=255)

                  aaTCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTa                                                                          >  1:4010416/1‑91 (MQ=255)

                  aaTCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTa                                                                          >  1:1626763/1‑91 (MQ=255)

                     cccACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGAc                                                                       >  1:3829535/1‑91 (MQ=255)

                       caccacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGt                                                                     >  1:2037474/1‑91 (MQ=255)

                       caccacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGt                                                                     >  1:2037135/1‑91 (MQ=255)

                       caccacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGt                                                                     >  1:801903/1‑91 (MQ=255)

                        accacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGta                                                                    >  1:1828081/1‑91 (MQ=255)

                        accacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGta                                                                    >  1:1605307/1‑91 (MQ=255)

                        accacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGta                                                                    >  1:1828041/1‑91 (MQ=255)

                        accacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGta                                                                    <  1:2375272/91‑1 (MQ=255)

                         ccacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGtat                                                                   <  1:1684503/91‑1 (MQ=255)

                          cacTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGtata                                                                  >  1:3695008/1‑91 (MQ=255)

                           acTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATaa                                                                 <  1:3990562/91‑1 (MQ=255)

                           acTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATaa                                                                 >  1:397279/1‑91 (MQ=255)

                            cTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGAAGTATAAc                                                                >  1:2024102/1‑91 (MQ=255)

                             tCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATATTTTTTTTACTATACTTCTGTAGACGTATAACt                                                               >  1:262776/1‑91 (MQ=255)

                                  tACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTatttt                                                          <  1:3377057/91‑1 (MQ=255)

                                   aCTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttt                                                         <  1:3473291/91‑1 (MQ=255)

                                    cTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTatttttt                                                        <  1:3503475/91‑1 (MQ=255)

                                     tATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGGCGTATAACTatttttta                                                       <  1:831256/91‑1 (MQ=255)

                                     tATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTatttttta                                                       <  1:1821260/91‑1 (MQ=255)

                                       ttCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatc                                                     <  1:1337000/91‑1 (MQ=255)

                                            tACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatcatttt                                                <  1:1396890/91‑1 (MQ=255)

                                             aCATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatcattttt                                               >  1:1081379/1‑91 (MQ=255)

                                              cATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatcatttttt                                              <  1:3238119/91‑1 (MQ=255)

                                              cATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatcatttttt                                              >  1:537730/1‑91 (MQ=255)

                                              cATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTattttttatcatttttt                                              >  1:537715/1‑91 (MQ=255)

                                                    ttGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATctct                                        >  1:1920643/1‑91 (MQ=255)

                                                    ttGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATctct                                        <  1:2264343/91‑1 (MQ=255)

                                                    ttGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATctct                                        >  1:1350316/1‑91 (MQ=255)

                                                      gTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTag                                      <  1:52801/91‑1 (MQ=255)

                                                      gTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTag                                      >  1:2245874/1‑91 (MQ=255)

                                                      gTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTag                                      <  1:710318/91‑1 (MQ=255)

                                                       tCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTaga                                     >  1:2469855/1‑91 (MQ=255)

                                                        cTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTagag                                    >  1:95874/1‑91 (MQ=255)

                                                        cTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTagag                                    >  1:2823106/1‑91 (MQ=255)

                                                        cTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTagag                                    >  1:1997551/1‑91 (MQ=255)

                                                         tGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAgaga                                   <  1:160550/91‑1 (MQ=255)

                                                         tGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTAACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAgaga                                   >  1:3509170/1‑91 (MQ=255)

                                                         tGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTAACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAgaga                                   >  1:3509154/1‑91 (MQ=255)

                                                          gTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGaa                                  >  1:2621560/1‑91 (MQ=255)

                                                          gTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGaa                                  <  1:2246084/91‑1 (MQ=255)

                                                           ttttCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAat                                 >  1:1455334/1‑91 (MQ=255)

                                                           ttttCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAat                                 >  1:3184493/1‑91 (MQ=255)

                                                            tttCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAata                                >  1:2448815/1‑91 (MQ=255)

                                                                tctATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAAtatcta                            >  1:2757469/1‑91 (MQ=255)

                                                                tctATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAAtatcta                            >  1:198097/1‑91 (MQ=255)

                                                                 ctATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAAtatctat                           <  1:613865/91‑1 (MQ=255)

                                                                  tatttattATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAAtatctatc                          <  1:1374865/91‑1 (MQ=255)

                                                                  tatttattATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAAtatctatc                          <  1:1374948/91‑1 (MQ=255)

                                                                    tttattATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTg                        <  1:923004/91‑1 (MQ=255)

                                                                        ttatATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAtt                    <  1:3368093/91‑1 (MQ=255)

                                                                        ttatATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAtt                    >  1:2246612/1‑91 (MQ=255)

                                                                         tatataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttt                   <  1:216588/91‑1 (MQ=255)

                                                                          gtataGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAtttt                  >  1:2445600/2‑91 (MQ=255)

                                                                          atataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAtttt                  >  1:627498/1‑91 (MQ=255)

                                                                          atataGTAAAATAATTTTTTTACTATACTTCTGTAGAAGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAtttt                  >  1:3491356/1‑91 (MQ=255)

                                                                           tataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 <  1:2690426/91‑1 (MQ=255)

                                                                           tataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 <  1:2690446/91‑1 (MQ=255)

                                                                           tataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 <  1:632937/91‑1 (MQ=255)

                                                                           tataGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATATCTAGAGAATATCTATCTGTAttttt                 >  1:263482/1‑91 (MQ=255)

                                                                           tataGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 >  1:128177/1‑91 (MQ=255)

                                                                           tataGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 >  1:127867/1‑91 (MQ=255)

                                                                           tataGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTAttttt                 >  1:127850/1‑91 (MQ=255)

                                                                            ataGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTg                >  1:2671789/1‑91 (MQ=255)

                                                                             taGTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGa               >  1:3299646/1‑91 (MQ=255)

                                                                              aGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGAt              <  1:761873/91‑1 (MQ=255)

                                                                               gTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATa             >  1:2814215/1‑91 (MQ=255)

                                                                               gTAAAAAAATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATa             >  1:1306068/1‑91 (MQ=255)

                                                                                tAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATaa            >  1:3935937/1‑91 (MQ=255)

                                                                                 aaaaaaATTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAac           >  1:140638/1‑91 (MQ=255)

                                                                                 aaaaTAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAac           <  1:1700475/91‑1 (MQ=255)

                                                                                 aaaaTAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAac           >  1:323783/1‑91 (MQ=255)

                                                                                  aaaTAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAacc          >  1:1010339/1‑91 (MQ=255)

                                                                                  aaaTAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAacc          <  1:3587105/91‑1 (MQ=255)

                                                                                   aataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAacca         >  1:1396269/1‑91 (MQ=255)

                                                                                   aataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAacca         >  1:2318700/1‑91 (MQ=255)

                                                                                   aataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAacca         <  1:1636176/91‑1 (MQ=255)

                                                                                    ataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAaccac        >  1:1233301/1‑91 (MQ=255)

                                                                                    ataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAaccac        <  1:2833851/91‑1 (MQ=255)

                                                                                    ataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAaccac        <  1:2833766/91‑1 (MQ=255)

                                                                                    ataatTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAaccac        <  1:767160/91‑1 (MQ=255)

                                                                                    aaaatTATTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAaccac        >  1:596189/3‑91 (MQ=255)

                                                                                       atTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCAt     >  1:3925450/1‑91 (MQ=255)

                                                                                        tttttttACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCAtt    >  1:2879679/1‑91 (MQ=255)

                                                                                         ttttttACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCAttt   >  1:3017700/1‑91 (MQ=255)

                                                                                          tttttACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCATTTg  >  1:1913577/1‑91 (MQ=255)

                                                                                          tttttACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCATTTg  >  1:1032306/1‑91 (MQ=255)

CTTTTAGACGAACGTTTCAATCCCACCACTCCTTTACTATTCCTTACATACTTTGTCTGTTTTCTCTATTTATTATATAGTAAAATAATTTTTTTACTATACTTCTGTAGACGTATAACTATTTTTTATCATTTTTTATCTCTAGAGAATATCTATCTGTATTTTTGATAACCACCATTTG  >  USA300TCH1516_ALE/2429627‑2429807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`