BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,668,296	A→C	5.3%	intergenic (‑105/‑446)	USA300HOU_RS13735 ← / → USA300TCH1516_02539	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,668,296	0	A	C	5.3%	398.7 / 11.8	113	intergenic (‑105/‑446)	USA300HOU_RS13735/USA300TCH1516_02539	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (44/63); new base C (3/3); total (47/66)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 117 total aligned reads displayed.

TCTCTATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTT  >  USA300TCH1516_ALE/2668206‑2668385

tctctATGTTTATTTTATTTTCAGACCATAATCATCGTACACCCTTAAGAAAACGTTGTCAACTTTTATCTGTTATCAAATCAAATATTTa                                                                                           >  1:3683043/1‑91 (MQ=255)

tctctATGTTTATTTTATTTTCAGACCATAACCATCGTACACCCTTAAGAAAACGTTCTCAACTTTTATCTGTTATCAAATCAAATATTTa                                                                                           >  1:3684234/1‑91 (MQ=255)

tctctATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTa                                                                                           >  1:2970773/1‑91 (MQ=255)

tctctATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTa                                                                                           >  1:658166/1‑91 (MQ=255)

  tctATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAg                                                                                         <  1:1352074/91‑1 (MQ=255)

  tctACGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAg                                                                                         <  1:3455917/91‑1 (MQ=255)

     aTGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGa                                                                                      <  1:3651373/91‑1 (MQ=255)

          tattttatttTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatat                                                                                 <  1:2211682/91‑1 (MQ=255)

           attttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatt                                                                                <  1:3700172/91‑1 (MQ=255)

            ttttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatattt                                                                               <  1:18808/91‑1 (MQ=255)

              ttattttCAGACCATAATCATCGTACACCCTTGAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatttga                                                                             <  1:3552516/91‑1 (MQ=38)

              ttattttCAGACCATAAGCATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatttga                                                                             <  1:3552369/91‑1 (MQ=38)

              ttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttga                                                                             <  1:1004928/91‑1 (MQ=255)

              ttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttga                                                                             <  1:1003726/91‑1 (MQ=255)

               tattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgat                                                                            <  1:2318146/91‑1 (MQ=255)

                attttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgata                                                                           >  1:573771/1‑91 (MQ=255)

                 ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                          >  1:2505574/1‑91 (MQ=255)

                 ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                          >  1:1782808/1‑91 (MQ=255)

                 ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                          >  1:205217/1‑91 (MQ=255)

                 ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                          >  1:204653/1‑91 (MQ=255)

                 ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                          >  1:1319324/1‑91 (MQ=255)

                     cAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgt                                                                      <  1:3522164/91‑1 (MQ=255)

                      aGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgtg                                                                     >  1:3369864/1‑91 (MQ=255)

                        aCCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGaa                                                                   <  1:450685/91‑1 (MQ=255)

                         ccATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGaaa                                                                  >  1:295900/1‑91 (MQ=255)

                                  tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                         <  1:1463660/91‑1 (MQ=255)

                                  tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                         <  1:1464044/91‑1 (MQ=255)

                                  tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                         >  1:730510/1‑91 (MQ=255)

                                  tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                         <  1:2467388/91‑1 (MQ=255)

                                   cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                        >  1:2865997/1‑91 (MQ=255)

                                   cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                        >  1:2826504/1‑91 (MQ=255)

                                   cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                        >  1:718214/1‑91 (MQ=255)

                                    gTACACCCTTAAGAAAACGTTTTCAACTTTTATGTGTGATCAAATCCAATATTTAAGTGCAATATTTGATATTTGTGAAAGATTTTTaaaa                                                       >  1:3308342/1‑91 (MQ=255)

                                    gTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaaa                                                       >  1:3585226/1‑91 (MQ=255)

                                     tACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAAt                                                      <  1:337270/91‑1 (MQ=255)

                                     tACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAAt                                                      <  1:254288/91‑1 (MQ=255)

                                      acacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATg                                                     >  1:2858078/1‑91 (MQ=255)

                                       cacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATgg                                                    >  1:4000004/1‑91 (MQ=255)

                                       cacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATgg                                                    >  1:3999721/1‑91 (MQ=255)

                                        acCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGa                                                   >  1:3765997/1‑91 (MQ=255)

                                        acCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGa                                                   >  1:2920300/1‑91 (MQ=255)

                                         cccTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGAt                                                  <  1:643751/91‑1 (MQ=255)

                                           cTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTg                                                <  1:3994672/91‑1 (MQ=255)

                                             tAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGtt                                              <  1:734992/91‑1 (MQ=255)

                                              aaGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGttt                                             <  1:468647/91‑1 (MQ=255)

                                               aGAAATCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTc                                            <  1:2037159/91‑1 (MQ=255)

                                               aGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTc                                            <  1:2037462/91‑1 (MQ=255)

                                                gAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCa                                           <  1:358629/91‑1 (MQ=255)

                                                   aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                        <  1:1981687/91‑1 (MQ=255)

                                                   aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                        <  1:1981572/91‑1 (MQ=255)

                                                   aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                        <  1:3920298/91‑1 (MQ=255)

                                                   aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                        <  1:3472472/91‑1 (MQ=255)

                                                     cGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAac                                      >  1:1624671/1‑91 (MQ=255)

                                                      gTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAact                                     <  1:3995132/91‑1 (MQ=255)

                                                       ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                    >  1:861182/1‑91 (MQ=255)

                                                       ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                    >  1:405092/1‑91 (MQ=255)

                                                       ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                    >  1:2430806/1‑91 (MQ=255)

                                                       ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                    <  1:28819/91‑1 (MQ=255)

                                                        tttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAactac                                   >  1:1587065/1‑91 (MQ=255)

                                                           cAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTa                                <  1:1566337/91‑1 (MQ=255)

                                                           cAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTa                                <  1:3939955/91‑1 (MQ=255)

                                                               ttttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgtt                            <  1:4010975/91‑1 (MQ=255)

                                                               ttttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgtt                            <  1:1875351/91‑1 (MQ=255)

                                                                tttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGTTTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttg                           <  1:763185/91‑1 (MQ=255)

                                                                 ttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttgt                          >  1:391814/1‑91 (MQ=255)

                                                                 ttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttgt                          <  1:3630798/91‑1 (MQ=255)

                                                                    tCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGt                       <  1:3641443/91‑1 (MQ=255)

                                                                     cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                      <  1:1472579/91‑1 (MQ=255)

                                                                     cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                      >  1:2863297/1‑91 (MQ=255)

                                                                     cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                      <  1:2938533/91‑1 (MQ=255)

                                                                     cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                      <  1:1478361/91‑1 (MQ=255)

                                                                     cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGATTCAAAAACTACTTATGTTGTCGtt                      >  1:3847826/1‑91 (MQ=255)

                                                                        ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                   <  1:857891/91‑1 (MQ=255)

                                                                        ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                   >  1:2827143/1‑91 (MQ=255)

                                                                        ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                   <  1:857462/91‑1 (MQ=255)

                                                                          atcaaatcaaATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAatat                 <  1:808777/91‑1 (MQ=255)

                                                                            caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt               <  1:3245832/91‑1 (MQ=255)

                                                                            caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt               <  1:3245807/91‑1 (MQ=255)

                                                                            caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt               >  1:2493687/1‑91 (MQ=255)

                                                                               atcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACt            >  1:2811448/1‑91 (MQ=255)

                                                                                tcaaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTa           >  1:2343554/1‑91 (MQ=39)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          <  1:3808748/91‑1 (MQ=255)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          <  1:1571883/91‑1 (MQ=255)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          <  1:2965407/91‑1 (MQ=255)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          >  1:128633/1‑91 (MQ=255)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          <  1:3794980/91‑1 (MQ=255)

                                                                                 caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa          <  1:3796865/91‑1 (MQ=255)

                                                                                  aaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAt         >  1:3839148/1‑91 (MQ=39)

                                                                                  aaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAt         >  1:1528466/1‑91 (MQ=39)

                                                                                   aatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAtt        <  1:1897283/91‑1 (MQ=255)

                                                                                   aatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAtt        <  1:2719719/91‑1 (MQ=255)

                                                                                     tatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTaa      >  1:1858422/1‑91 (MQ=255)

                                                                                     tatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTaa      <  1:1696327/91‑1 (MQ=255)

                                                                                      atTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAAc     >  1:3401539/1‑91 (MQ=255)

                                                                                      atTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAAc     >  1:2807093/1‑91 (MQ=255)

                                                                                      atTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATCTTGTCGTTAATATTTACTAATTAAc     >  1:3864516/1‑91 (MQ=255)

                                                                                        ttAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACtt   >  1:1831615/1‑91 (MQ=255)

                                                                                        ttAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACtt   <  1:3606883/91‑1 (MQ=255)

                                                                                         tAAGTGAACTATTTGATATTTGTGAAAGATTTTTAAAATGGATTATTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACttt  <  1:3296919/91‑1 (MQ=255)

                                                                                         tAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACttt  <  1:1268586/91‑1 (MQ=255)

TCTCTATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTT  >  USA300TCH1516_ALE/2668206‑2668385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`