BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,668,300	G→T	5.3%	intergenic (‑109/‑442)	USA300HOU_RS13735 ← / → USA300TCH1516_02539	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,668,300	0	G	T	5.3%	404.9 / 11.0	114	intergenic (‑109/‑442)	USA300HOU_RS13735/USA300TCH1516_02539	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (45/63); new base T (3/3); total (48/66)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 114 total aligned reads displayed.

ATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTTTTAC  >  USA300TCH1516_ALE/2668211‑2668389

aTGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGa                                                                                          <  1:3651373/91‑1 (MQ=255)

      attttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatt                                                                                    <  1:3700172/91‑1 (MQ=255)

       ttttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatattt                                                                                   <  1:18808/91‑1 (MQ=255)

        tttattttCAGACCATAAACATCGTGCACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttg                                                                                  <  1:3857631/91‑1 (MQ=255)

         ttattttCAGACCATAATCATCGTACACCCTTGAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatttga                                                                                 <  1:3552516/91‑1 (MQ=38)

         ttattttCAGACCATAAGCATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTCACTTAAatatttga                                                                                 <  1:3552369/91‑1 (MQ=38)

         ttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttga                                                                                 <  1:1004928/91‑1 (MQ=255)

         ttattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttga                                                                                 <  1:1003726/91‑1 (MQ=255)

          tattttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgat                                                                                <  1:2318146/91‑1 (MQ=255)

           attttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgata                                                                               >  1:573771/1‑91 (MQ=255)

            ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                              >  1:2505574/1‑91 (MQ=255)

            ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                              >  1:1319324/1‑91 (MQ=255)

            ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                              >  1:205217/1‑91 (MQ=255)

            ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                              >  1:204653/1‑91 (MQ=255)

            ttttCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAatatttgatat                                                                              >  1:1782808/1‑91 (MQ=255)

                cAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgt                                                                          <  1:375866/91‑1 (MQ=255)

                cAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgt                                                                          <  1:3522164/91‑1 (MQ=255)

                 aGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgtg                                                                         >  1:3369864/1‑91 (MQ=255)

                 aGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgtg                                                                         >  1:1454542/1‑91 (MQ=255)

                 aGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTtgtg                                                                         <  1:2000089/91‑1 (MQ=255)

                   aCCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGaa                                                                       <  1:450685/91‑1 (MQ=255)

                    ccATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGaaa                                                                      >  1:295900/1‑91 (MQ=255)

                      aTAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGa                                                                    >  1:3624387/1‑91 (MQ=255)

                      aTAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGa                                                                    <  1:2703300/91‑1 (MQ=255)

                          aCATCGTACACCCTTAAGAAAACGTTTTCAACTTTTAACTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGAtttt                                                                >  1:3497269/1‑91 (MQ=255)

                             tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                             <  1:1463660/91‑1 (MQ=255)

                             tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                             <  1:1464044/91‑1 (MQ=255)

                             tCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaa                                                             <  1:2467388/91‑1 (MQ=255)

                              cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                            >  1:2865997/1‑91 (MQ=255)

                              cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                            >  1:2826504/1‑91 (MQ=255)

                              cGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaa                                                            >  1:718214/1‑91 (MQ=255)

                               gTACACCCTTAAGAAAACGTTTTCAACTTTTATGTGTGATCAAATCCAATATTTAAGTGCAATATTTGATATTTGTGAAAGATTTTTaaaa                                                           >  1:3308342/1‑91 (MQ=255)

                               gTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTaaaa                                                           >  1:3585226/1‑91 (MQ=255)

                                tACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAAt                                                          <  1:337270/91‑1 (MQ=255)

                                 acacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATg                                                         >  1:1567939/1‑91 (MQ=255)

                                  cacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATgg                                                        >  1:3999721/1‑91 (MQ=255)

                                  cacCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATgg                                                        >  1:4000004/1‑91 (MQ=255)

                                   acCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGa                                                       >  1:2920300/1‑91 (MQ=255)

                                    cccTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGAt                                                      <  1:643751/91‑1 (MQ=255)

                                      cTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTg                                                    <  1:3994672/91‑1 (MQ=255)

                                        tAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGtt                                                  <  1:734992/91‑1 (MQ=255)

                                         aaGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGttt                                                 <  1:468647/91‑1 (MQ=255)

                                          aGAAATCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTc                                                <  1:2037159/91‑1 (MQ=255)

                                          aGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTc                                                <  1:2037462/91‑1 (MQ=255)

                                             aaaCGTTTTCAACTTTTATCTGTTATCAAATCAAATACTTAAGTGAGATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaa                                             >  1:3303521/1‑91 (MQ=255)

                                              aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                            <  1:3920298/91‑1 (MQ=255)

                                              aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                            <  1:3472472/91‑1 (MQ=255)

                                              aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                            <  1:1981687/91‑1 (MQ=255)

                                              aaCGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCaaaa                                            <  1:1981572/91‑1 (MQ=255)

                                                 gTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAact                                         <  1:3995132/91‑1 (MQ=255)

                                                 gTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAact                                         <  1:3995573/91‑1 (MQ=255)

                                                  ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                        >  1:861182/1‑91 (MQ=255)

                                                  ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                        >  1:405092/1‑91 (MQ=255)

                                                  ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                        >  1:2430806/1‑91 (MQ=255)

                                                  ttttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAacta                                        <  1:28819/91‑1 (MQ=255)

                                                   tttCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAactac                                       >  1:1587065/1‑91 (MQ=255)

                                                      cAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTa                                    <  1:3939955/91‑1 (MQ=255)

                                                      cAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTa                                    <  1:1566337/91‑1 (MQ=255)

                                                          ttttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgtt                                <  1:1875351/91‑1 (MQ=255)

                                                           tttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGTTTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttg                               <  1:763185/91‑1 (MQ=255)

                                                           tttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttg                               <  1:763150/91‑1 (MQ=255)

                                                            ttATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTAtgttgt                              <  1:3630798/91‑1 (MQ=255)

                                                               tCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGt                           <  1:3641443/91‑1 (MQ=255)

                                                                cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                          <  1:2938533/91‑1 (MQ=255)

                                                                cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                          >  1:2863297/1‑91 (MQ=255)

                                                                cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGtt                          <  1:1478361/91‑1 (MQ=255)

                                                                cTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGATTCAAAAACTACTTATGTTGTCGtt                          >  1:3847826/1‑91 (MQ=255)

                                                                   ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                       >  1:2827143/1‑91 (MQ=255)

                                                                   ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                       <  1:857462/91‑1 (MQ=255)

                                                                   ttATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAat                       <  1:857891/91‑1 (MQ=255)

                                                                     atcaaatcaaATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAatat                     <  1:808777/91‑1 (MQ=255)

                                                                       caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt                   <  1:3245832/91‑1 (MQ=255)

                                                                       caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt                   >  1:2493687/1‑91 (MQ=255)

                                                                       caaatcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATAttt                   <  1:3245807/91‑1 (MQ=255)

                                                                          atcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACt                >  1:2811448/1‑91 (MQ=255)

                                                                          atcaaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACt                >  1:2862156/1‑91 (MQ=255)

                                                                           tcaaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTa               >  1:2343554/1‑91 (MQ=39)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              <  1:3796865/91‑1 (MQ=255)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              >  1:128633/1‑91 (MQ=255)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              <  1:1571883/91‑1 (MQ=255)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              <  1:3794980/91‑1 (MQ=255)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              <  1:2965407/91‑1 (MQ=255)

                                                                            caaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTaa              <  1:3808748/91‑1 (MQ=255)

                                                                             aaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAt             >  1:3839148/1‑91 (MQ=39)

                                                                             aaatATTTCACTTAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAt             >  1:1528466/1‑91 (MQ=39)

                                                                             aaatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAt             <  1:2341136/91‑1 (MQ=255)

                                                                              aatATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAAtt            <  1:2719719/91‑1 (MQ=255)

                                                                               atatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTa           >  1:52474/1‑91 (MQ=255)

                                                                                tatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTaa          >  1:1858422/1‑91 (MQ=255)

                                                                                tatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTaa          >  1:1858366/1‑91 (MQ=255)

                                                                                tatTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTaa          <  1:1696327/91‑1 (MQ=255)

                                                                                 atTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAAc         >  1:2807093/1‑91 (MQ=255)

                                                                                 atTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATCTTGTCGTTAATATTTACTAATTAAc         >  1:3864516/1‑91 (MQ=255)

                                                                                   ttAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACtt       <  1:3606883/91‑1 (MQ=255)

                                                                                   ttAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACtt       >  1:1831615/1‑91 (MQ=255)

                                                                                    tAAGTGAACTATTTGATATTTGTGAAAGATTTTTAAAATGGATTATTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACttt      <  1:3296919/91‑1 (MQ=255)

                                                                                    tAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACttt      <  1:1268586/91‑1 (MQ=255)

                                                                                      aGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACttttt    >  1:2678928/1‑91 (MQ=255)

                                                                                        tGAAATATTTGATATTTGTGAAAGATTTTTAATATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTTTTAc  <  1:3538498/91‑1 (MQ=255)

                                                                                        tGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTTTTAc  <  1:1558277/91‑1 (MQ=255)

ATGTTTATTTTATTTTCAGACCATAAACATCGTACACCCTTAAGAAAACGTTTTCAACTTTTATCTGTTATCAAATCAAATATTTAAGTGAAATATTTGATATTTGTGAAAGATTTTTAAAATGGATTGTTTCAAAAACTACTTATGTTGTCGTTAATATTTACTAATTAACTTTTTAC  >  USA300TCH1516_ALE/2668211‑2668389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`