Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,745,325 T→A 5.6% N143I (AAT→ATT)  mqo2 ← putative malate:quinone oxidoreductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,745,3250TA 5.6% 313.5 / 7.7 90N143I (AAT→ATT) mqo2putative malate:quinone oxidoreductase 2
Reads supporting (aligned to +/- strand):  ref base T (43/42);  new base A (3/2);  total (46/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

GCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGT  >  USA300TCH1516_ALE/2745235‑2745414
                                                                                          |                                                                                         
gccgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGatat                                                                                           <  1:2236462/91‑1 (MQ=255)
  cgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGataata                                                                                         <  1:1148619/91‑1 (MQ=255)
  cgccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGAtatta                                                                                         <  1:1561015/91‑1 (MQ=255)
    ccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATc                                                                                       <  1:291901/91‑1 (MQ=255)
    ccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATc                                                                                       <  1:1461897/91‑1 (MQ=255)
    ccATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATAATATc                                                                                       <  1:3169916/91‑1 (MQ=255)
       tGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGaa                                                                                    >  1:2530702/1‑91 (MQ=255)
       tGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGaa                                                                                    >  1:3026514/1‑91 (MQ=255)
       tGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGaa                                                                                    >  1:2776148/1‑91 (MQ=255)
       tGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGaa                                                                                    >  1:3026870/1‑91 (MQ=255)
       tGATACCAGAGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGaa                                                                                    >  1:2093025/1‑91 (MQ=255)
        gATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAAc                                                                                   <  1:1232761/91‑1 (MQ=255)
         aTACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCTATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACa                                                                                  <  1:3367190/91‑1 (MQ=255)
          tACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACAt                                                                                 >  1:842399/1‑91 (MQ=255)
          tACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACAt                                                                                 >  1:3160522/1‑91 (MQ=255)
            ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATGTTCGATAATATCGAAtatac                                                                               <  1:1567310/91‑2 (MQ=39)
            ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAg                                                                               <  1:3076238/91‑1 (MQ=255)
            ccAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAg                                                                               >  1:3787201/1‑91 (MQ=255)
              aGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAggg                                                                             >  1:372043/1‑91 (MQ=255)
               gggTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGa                                                                            <  1:3776496/91‑1 (MQ=255)
               gggTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGa                                                                            <  1:1950668/91‑1 (MQ=255)
                ggTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGaa                                                                           <  1:2630035/91‑1 (MQ=255)
                ggTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGaa                                                                           <  1:2629994/91‑1 (MQ=255)
                  ttATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                         >  1:3025474/1‑91 (MQ=255)
                  ttATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                         >  1:3025570/1‑91 (MQ=255)
                  ttATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                         >  1:1305188/1‑91 (MQ=255)
                  ttATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAg                                                                         <  1:2713981/91‑1 (MQ=255)
                   tATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGc                                                                        <  1:2115331/91‑1 (MQ=255)
                   tATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGc                                                                        <  1:1901934/91‑1 (MQ=255)
                    aTCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCt                                                                       <  1:789347/91‑1 (MQ=255)
                     tCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCtt                                                                      <  1:2666824/91‑1 (MQ=255)
                     tCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCtt                                                                      <  1:2346891/91‑1 (MQ=255)
                     tCTTCACGGCCTTTCATCATCAATGGAATCCATGTTTTCATTACTTCGATGTCTTCAGTGTATTCGATATTATCGAACATAGGGAAAGCtt                                                                      <  1:3681482/91‑1 (MQ=255)
                      cTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCttt                                                                     <  1:1421118/91‑1 (MQ=255)
                      cTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCttt                                                                     >  1:2574304/1‑91 (MQ=255)
                      cTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCttt                                                                     >  1:679897/1‑91 (MQ=255)
                      cTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCttt                                                                     >  1:1172841/1‑91 (MQ=255)
                       ttCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTc                                                                    >  1:3037779/1‑91 (MQ=255)
                         cACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCAt                                                                  <  1:527731/91‑1 (MQ=255)
                          aCGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATc                                                                 >  1:2886344/1‑91 (MQ=255)
                          aCGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATc                                                                 <  1:1045801/91‑1 (MQ=255)
                             gCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCt                                                              >  1:2504066/1‑91 (MQ=255)
                             gCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCt                                                              >  1:3068547/1‑91 (MQ=255)
                                 ttCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGt                                                          <  1:3946080/91‑1 (MQ=255)
                                  tcatcatcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTa                                                         >  1:2190461/1‑91 (MQ=255)
                                    atcatcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAAc                                                       <  1:1967831/91‑1 (MQ=255)
                                      catcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGa                                                     >  1:2441507/1‑91 (MQ=255)
                                      catcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGa                                                     <  1:2061944/91‑1 (MQ=255)
                                       atcaATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGAt                                                    >  1:3660007/1‑91 (MQ=255)
                                         caATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCt                                                  >  1:262625/1‑91 (MQ=255)
                                          aaTGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCtt                                                 <  1:212698/91‑1 (MQ=255)
                                          aaTGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCtt                                                 <  1:1483353/91‑1 (MQ=255)
                                          aaTGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCtt                                                 >  1:823397/1‑91 (MQ=255)
                                                aTCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGa                                           >  1:2787788/1‑91 (MQ=255)
                                                   cATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAtt                                        >  1:2818549/1‑91 (MQ=255)
                                                   cATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAtt                                        >  1:2818564/1‑91 (MQ=255)
                                                   cATTTTTTCATTACTTCGACGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAtt                                        >  1:4104787/1‑91 (MQ=255)
                                                    aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAttt                                       <  1:2434178/91‑1 (MQ=255)
                                                    aTTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAAttt                                       <  1:1470236/91‑1 (MQ=255)
                                                       ttttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAAc                                    >  1:1009114/1‑91 (MQ=255)
                                                        tttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACa                                   >  1:3992960/1‑91 (MQ=255)
                                                        tttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACa                                   <  1:720679/91‑1 (MQ=255)
                                                        tttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACa                                   >  1:1284333/1‑91 (MQ=255)
                                                         ttCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACAt                                  <  1:32832/91‑1 (MQ=255)
                                                          tCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACAtt                                 >  1:2161758/1‑91 (MQ=255)
                                                          tCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACAtt                                 >  1:2161553/1‑91 (MQ=255)
                                                            aTTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGt                               >  1:1009374/1‑91 (MQ=255)
                                                             ttACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGtt                              <  1:3848427/91‑1 (MQ=255)
                                                             ttACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGtt                              <  1:1771414/91‑1 (MQ=255)
                                                                cTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGttttt                           <  1:3092784/91‑1 (MQ=255)
                                                                        tCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTaa                   <  1:2269209/91‑1 (MQ=255)
                                                                         cTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAAc                  <  1:971723/91‑1 (MQ=255)
                                                                         cTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAAc                  <  1:971722/91‑1 (MQ=255)
                                                                         cTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAAc                  <  1:2934564/91‑1 (MQ=255)
                                                                          ttCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACa                 <  1:1301683/91‑1 (MQ=255)
                                                                          ttCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACa                 <  1:1600766/91‑1 (MQ=255)
                                                                            cAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATa               >  1:2865960/1‑91 (MQ=255)
                                                                            cAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATa               >  1:4098383/1‑91 (MQ=255)
                                                                              gTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAAc             <  1:2842447/91‑1 (MQ=255)
                                                                               tataTTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACt            <  1:3517446/91‑1 (MQ=255)
                                                                                atatTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTg           <  1:1294852/91‑1 (MQ=255)
                                                                                 tatTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGa          >  1:646174/1‑91 (MQ=255)
                                                                                 tatTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGa          >  1:646048/1‑91 (MQ=255)
                                                                                  atTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAt         >  1:1691188/1‑91 (MQ=255)
                                                                                  atTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAt         <  1:4034962/91‑1 (MQ=255)
                                                                                   ttCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtg        >  1:1211512/1‑91 (MQ=255)
                                                                                   ttCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtg        >  1:1211781/1‑91 (MQ=255)
                                                                                   ttCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtg        >  1:179644/1‑91 (MQ=255)
                                                                                   ttCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtg        >  1:2526114/1‑91 (MQ=255)
                                                                                   ttCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtg        <  1:306609/91‑1 (MQ=255)
                                                                                     cGATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtgtg      >  1:335284/1‑91 (MQ=255)
                                                                                      gATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtgtgt     >  1:2350026/1‑91 (MQ=255)
                                                                                      gATAATATCGAATATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGAtgtgt     >  1:3456738/1‑91 (MQ=255)
                                                                                        tattatCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg   <  1:2241706/91‑1 (MQ=255)
                                                                                        tattatCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg   <  1:270024/91‑1 (MQ=255)
                                                                                        tattatCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg   <  1:2730484/91‑1 (MQ=255)
                                                                                        tattatCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTgg   <  1:3734633/91‑1 (MQ=255)
                                                                                         attatCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGt  <  1:3359752/91‑1 (MQ=255)
                                                                                          |                                                                                         
GCCGCCATGATACCAGGGTTATCTTCACGGCCTTTCATCATCAATGGAATCCATTTTTTCATTACTTCGATGTCTTCAGTATATTCGATATTATCGAACATAGGGAAAGCTTTCATCGCTTCGTAACGATCTTTTAAGAATTTAACATTGTTTTTACCTCTAACATAACTGATGTGTGGT  >  USA300TCH1516_ALE/2745235‑2745414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑