BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,783,163	T→A	9.0%	intergenic (‑68/+279)	arcA_2 ← / ← argR_3	Arginine deiminase/Arginine repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,783,163	0	T	A	9.0%	336.6 / 20.1	100	intergenic (‑68/+279)	arcA_2/argR_3	Arginine deiminase/Arginine repressor
Reads supporting (aligned to +/- strand): ref base T (50/41); new base A (3/6); total (53/47)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 101 total aligned reads displayed.

CTTTAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCATTTT  >  USA300TCH1516_ALE/2783074‑2783252

cTTTAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAtt                                                                                          <  1:2281610/91‑1 (MQ=255)

 tttAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATta                                                                                         <  1:1064455/91‑1 (MQ=255)

  ttAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATtat                                                                                        >  1:4053884/1‑91 (MQ=255)

   tAATTGTACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATtata                                                                                       >  1:3222974/1‑91 (MQ=255)

   tAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATtata                                                                                       <  1:2896769/91‑1 (MQ=255)

   tAATTGCACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATtata                                                                                       >  1:3222973/1‑91 (MQ=255)

   tAATTGAACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATtata                                                                                       >  1:3222845/1‑91 (MQ=255)

    aaTTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTatat                                                                                      >  1:1572318/1‑91 (MQ=255)

     aTTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAAtataat                                                                                     <  1:1972443/91‑3 (MQ=255)

     aTTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAAtataat                                                                                     <  1:1970049/91‑3 (MQ=255)

      ttGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAAtataatc                                                                                    <  1:3907274/91‑4 (MQ=255)

      ttGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAAtataatc                                                                                    <  1:1305460/91‑4 (MQ=255)

       tGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTca                                                                                   <  1:3073004/91‑1 (MQ=255)

       tGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAATATAATca                                                                                   <  1:400395/91‑1 (MQ=255)

         gACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTcaca                                                                                 <  1:2144166/91‑1 (MQ=255)

         gACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTcaca                                                                                 <  1:298530/91‑1 (MQ=255)

         gACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGAATATAATcaca                                                                                 <  1:1024497/91‑1 (MQ=255)

          aCCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACAt                                                                                >  1:3053728/1‑91 (MQ=255)

           ccATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATa                                                                               >  1:2016668/1‑91 (MQ=255)

            cATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaa                                                                              >  1:2355235/1‑91 (MQ=255)

                tGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaa                                                                          <  1:1927268/91‑1 (MQ=255)

                 gatctGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaa                                                                         <  1:2212840/87‑1 (MQ=255)

                 gTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaa                                                                         <  1:2500569/91‑1 (MQ=255)

                  tCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaag                                                                        <  1:2903978/91‑1 (MQ=255)

                  tCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaag                                                                        >  1:1880460/1‑91 (MQ=255)

                  tCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaag                                                                        <  1:3036729/91‑1 (MQ=255)

                  tCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaag                                                                        >  1:553080/1‑91 (MQ=255)

                  tCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATaaagaaag                                                                        >  1:3083788/1‑91 (MQ=255)

                   cATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGt                                                                       <  1:2800840/91‑1 (MQ=255)

                    aTGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTc                                                                      <  1:3521182/91‑1 (MQ=255)

                     tGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCg                                                                     >  1:3180462/1‑91 (MQ=255)

                      gTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGa                                                                    >  1:2723773/1‑91 (MQ=255)

                      gTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGa                                                                    <  1:249089/91‑1 (MQ=255)

                         tATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGaaaa                                                                 >  1:3143468/1‑91 (MQ=255)

                          aTTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAAt                                                                >  1:2243847/1‑91 (MQ=255)

                           tttCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAAtt                                                               >  1:759753/1‑91 (MQ=255)

                             tcctccTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTc                                                             <  1:3141440/91‑1 (MQ=255)

                               ctcctTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCat                                                           <  1:1240499/91‑1 (MQ=255)

                               ctcctTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCat                                                           >  1:3806184/1‑91 (MQ=255)

                                tcctTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCata                                                          >  1:3664743/1‑91 (MQ=255)

                                 cctTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCatat                                                         <  1:2464740/91‑1 (MQ=255)

                                    tttATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCtt                                                      <  1:1222752/91‑1 (MQ=255)

                                    tttATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCtt                                                      <  1:1253969/91‑1 (MQ=255)

                                    tttATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCtt                                                      <  1:1222443/91‑1 (MQ=255)

                                     ttATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCttt                                                     <  1:4014380/91‑1 (MQ=255)

                                       aTCTTTGAATTCATTTAGAGTATAGACAATCGTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAGTTTCATATCttttt                                                   >  1:3741956/1‑91 (MQ=255)

                                        tCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTg                                                  >  1:1394503/1‑91 (MQ=255)

                                           ttGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTta                                               <  1:788587/91‑1 (MQ=255)

                                           ttGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTta                                               >  1:3590762/1‑91 (MQ=255)

                                           ttGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTta                                               <  1:594385/91‑1 (MQ=255)

                                            tGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTtat                                              <  1:2418825/91‑1 (MQ=255)

                                              aaTTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatac                                            <  1:779733/91‑1 (MQ=255)

                                               aTTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTataca                                           >  1:465682/1‑91 (MQ=255)

                                               aTTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTataca                                           >  1:693735/1‑91 (MQ=255)

                                               aTTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTataca                                           >  1:693722/1‑91 (MQ=255)

                                                ttCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatacaa                                          >  1:3799197/1‑91 (MQ=255)

                                                  cATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatacaata                                        <  1:4088491/91‑1 (MQ=255)

                                                  cATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatacaata                                        >  1:2716697/1‑91 (MQ=255)

                                                  cATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatacaata                                        >  1:2716656/1‑91 (MQ=255)

                                                    tttAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTatacaataca                                      >  1:2662958/1‑91 (MQ=255)

                                                        gagTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACataat                                  >  1:280733/1‑91 (MQ=255)

                                                        gagTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACataat                                  >  1:2559180/1‑91 (MQ=255)

                                                          gTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACAtaataa                                <  1:2687821/91‑1 (MQ=255)

                                                          gTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACAtaataa                                <  1:1599246/91‑1 (MQ=255)

                                                          gTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACAtaataa                                <  1:2687787/91‑1 (MQ=255)

                                                            ataGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATg                              <  1:1434514/91‑1 (MQ=255)

                                                            ataGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATg                              <  1:3668364/91‑1 (MQ=255)

                                                            ataGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATg                              <  1:3667314/91‑1 (MQ=255)

                                                             taGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATgg                             >  1:2405294/1‑91 (MQ=255)

                                                             taGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATgg                             >  1:2405346/1‑91 (MQ=255)

                                                             taGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATgg                             >  1:415336/1‑91 (MQ=255)

                                                                aCAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGAtt                          >  1:3538277/1‑91 (MQ=255)

                                                                 cAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGAttt                         >  1:1541158/1‑91 (MQ=255)

                                                                 cAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGAttt                         >  1:2449492/1‑91 (MQ=255)

                                                                 cAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGAttt                         <  1:372380/91‑1 (MQ=255)

                                                                  aaTCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGAtttt                        >  1:2334391/1‑91 (MQ=255)

                                                                    tCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTta                      <  1:1608785/91‑1 (MQ=255)

                                                                    tCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTta                      <  1:346721/91‑1 (MQ=255)

                                                                    tCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTta                      <  1:3463461/91‑1 (MQ=255)

                                                                     cTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTtat                     >  1:202398/1‑91 (MQ=255)

                                                                         aaaCGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACat                 >  1:3999506/1‑91 (MQ=255)

                                                                            cGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACataaa              >  1:2687815/1‑91 (MQ=255)

                                                                            cGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACataaa              >  1:2687814/1‑91 (MQ=255)

                                                                                tACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACAtaaataaa          >  1:3703509/1‑91 (MQ=255)

                                                                                tACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACAtaaataaa          <  1:4084814/91‑1 (MQ=255)

                                                                                tACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACAtaaataaa          >  1:3671209/1‑91 (MQ=255)

                                                                                 ttaTGTGAATATAATCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAg         >  1:1471306/3‑91 (MQ=255)

                                                                                 aCATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAg         <  1:1117470/91‑1 (MQ=255)

                                                                                  cATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAgg        >  1:2175726/1‑91 (MQ=255)

                                                                                   aTGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGc       <  1:1750107/91‑1 (MQ=255)

                                                                                   aTGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGc       >  1:1775826/1‑91 (MQ=255)

                                                                                   aTGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGc       >  1:3446897/1‑91 (MQ=255)

                                                                                    tgtgAATATAATCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCa      >  1:3737321/1‑91 (MQ=255)

                                                                                     gtgATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAt     <  1:1722127/91‑1 (MQ=255)

                                                                                     gtgAATATAATCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAt     >  1:3619928/1‑91 (MQ=255)

                                                                                      tgATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAtt    >  1:1133404/1‑91 (MQ=255)

                                                                                      tgATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAtt    <  1:2128986/91‑1 (MQ=255)

                                                                                       gATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAttt   <  1:700095/91‑1 (MQ=255)

                                                                                        aTTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAtttt  >  1:4153876/1‑91 (MQ=255)

                                                                                        aTTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCAtttt  >  1:1517798/1‑91 (MQ=255)

CTTTAATTGGACCATCTGTCATGTCTATTTCCTCCTTTTATCTTTGAATTCATTTAGAGTATAGACAATCTTCAAACGCTTACATGTGATTATATTCACATAAAGAAAGTCGAAAATTTCATATCTTTTTGTTATACAATACATAATAATGGATTTTTATACATAAATAAAGGCATTTT  >  USA300TCH1516_ALE/2783074‑2783252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`