BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	413,758	G→A	5.8%	I167I (ATC→ATT)	metI ←	Cystathionine gamma‑synthase/O‑acetylhomoserine (thiol)‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	413,758	0	G	A	5.8%	349.2 / 7.8	104	I167I (ATC→ATT)	metI	Cystathionine gamma‑synthase/O‑acetylhomoserine (thiol)‑lyase
Reads supporting (aligned to +/- strand): ref base G (48/50); new base A (2/4); total (50/54)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 108 total aligned reads displayed.

TCCGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATG  >  USA300TCH1516_ALE/413668‑413848

tCCGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCg                                                                                            >  1:3523481/1‑91 (MQ=255)

tCCGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCg                                                                                            >  1:1180390/1‑91 (MQ=255)

tCCGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCg                                                                                            >  1:462486/1‑91 (MQ=255)

 ccGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGa                                                                                           <  1:3926179/91‑1 (MQ=255)

  cGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGAt                                                                                          <  1:3443636/91‑1 (MQ=255)

    ccAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATaa                                                                                        <  1:899108/91‑1 (MQ=255)

       atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                     <  1:2461929/91‑1 (MQ=255)

       atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                     <  1:2889159/91‑1 (MQ=255)

       atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                     <  1:3766451/91‑1 (MQ=255)

       atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                     >  1:1134217/1‑91 (MQ=255)

       atatatTTCGTCGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                     <  1:3766364/91‑1 (MQ=255)

         atatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGAc                                                                                   >  1:874519/1‑91 (MQ=255)

         atatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGAc                                                                                   <  1:681386/91‑1 (MQ=255)

           atTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACaa                                                                                 <  1:3149669/91‑1 (MQ=255)

            tttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAAt                                                                                >  1:1460896/1‑91 (MQ=255)

             ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                               <  1:2042469/91‑1 (MQ=255)

             ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                               <  1:2747204/91‑1 (MQ=255)

             ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                               <  1:585910/91‑1 (MQ=255)

              tCGTGGCTGAATGCAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATac                                                                              <  1:4142771/91‑2 (MQ=255)

                 tGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGcc                                                                           <  1:3918744/91‑1 (MQ=255)

                 tGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGcc                                                                           <  1:3918687/91‑1 (MQ=255)

                   gCTGAATGTAAGACTTTATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTACAAAAGTATTGTCGATAATTGACAATAAGCCtt                                                                         >  1:3536478/1‑91 (MQ=255)

                    cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGccctt                                                                        >  1:3823592/1‑88 (MQ=255)

                    cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCttt                                                                        >  1:13316/1‑91 (MQ=255)

                    cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCttt                                                                        <  1:2498963/91‑1 (MQ=255)

                      gAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAg                                                                      >  1:743187/1‑91 (MQ=255)

                        aTGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCt                                                                    <  1:3662885/91‑1 (MQ=255)

                         tGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCtt                                                                   >  1:3924072/1‑91 (MQ=255)

                         tGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCtt                                                                   >  1:3490650/1‑91 (MQ=255)

                          gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                  >  1:402064/1‑91 (MQ=255)

                          gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                  >  1:3437865/1‑91 (MQ=255)

                          gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                  >  1:3437152/1‑91 (MQ=255)

                           tAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTa                                                                 <  1:2456585/91‑1 (MQ=255)

                            aaGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTAc                                                                <  1:2715058/91‑1 (MQ=255)

                                cTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAg                                                            <  1:2165246/91‑1 (MQ=255)

                                cTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAg                                                            >  1:4123777/1‑91 (MQ=255)

                                 tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                           >  1:2590585/1‑91 (MQ=255)

                                 tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                           <  1:2867002/91‑1 (MQ=255)

                                 tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                           <  1:2867404/91‑1 (MQ=255)

                                     tCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGAt                                                       <  1:2247450/91‑1 (MQ=255)

                                       aGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGTTAg                                                     >  1:3350696/1‑91 (MQ=255)

                                        gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                    >  1:3773358/1‑91 (MQ=255)

                                        gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                    >  1:3637550/1‑91 (MQ=255)

                                        gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                    <  1:1464206/91‑1 (MQ=255)

                                         cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                   >  1:3063053/1‑91 (MQ=255)

                                         cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                   <  1:830187/91‑1 (MQ=255)

                                         cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                   <  1:830772/91‑1 (MQ=255)

                                          aCCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTAt                                                  <  1:3909153/91‑1 (MQ=255)

                                          aCCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTAt                                                  <  1:2259020/91‑1 (MQ=255)

                                            cTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATgg                                                <  1:253434/91‑1 (MQ=255)

                                             ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                               <  1:484259/91‑1 (MQ=255)

                                             ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                               >  1:1167502/1‑91 (MQ=255)

                                             ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                               <  1:1422241/91‑1 (MQ=255)

                                              tCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCt                                              <  1:848562/91‑1 (MQ=255)

                                               cTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCtt                                             >  1:4006402/1‑91 (MQ=255)

                                               cTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCtt                                             <  1:3805656/91‑1 (MQ=255)

                                                tGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCttt                                            >  1:3703452/1‑91 (MQ=255)

                                                tGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCttt                                            >  1:135531/1‑91 (MQ=255)

                                                 gCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTa                                           <  1:3392903/91‑1 (MQ=255)

                                                  cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                          <  1:570113/91‑1 (MQ=255)

                                                  cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                          >  1:3504245/1‑91 (MQ=255)

                                                  cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                          >  1:2573576/1‑91 (MQ=255)

                                                    aGTGGTGCTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACAt                                        <  1:2082145/91‑1 (MQ=255)

                                                     gtggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATc                                       <  1:1695965/91‑1 (MQ=255)

                                                     gtggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATc                                       <  1:1696307/91‑1 (MQ=255)

                                                      tggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCa                                      >  1:3151102/1‑91 (MQ=255)

                                                      tggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCa                                      >  1:1900904/1‑91 (MQ=255)

                                                         tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                   <  1:260371/91‑1 (MQ=255)

                                                         tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                   <  1:262060/91‑1 (MQ=255)

                                                         tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                   <  1:261798/91‑1 (MQ=255)

                                                          gtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAg                                  <  1:2231924/91‑1 (MQ=255)

                                                          gtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAg                                  <  1:823707/91‑1 (MQ=255)

                                                            tGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCa                                >  1:1851572/1‑91 (MQ=255)

                                                            tGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCa                                >  1:2954736/1‑91 (MQ=255)

                                                             gAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCaa                               <  1:3234485/91‑1 (MQ=255)

                                                              aaaGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAAt                              >  1:3673359/1‑91 (MQ=255)

                                                                 gATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAt                           >  1:1208886/1‑91 (MQ=255)

                                                                  aTAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAtt                          <  1:1273626/91‑1 (MQ=255)

                                                                   tAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAttt                         >  1:752718/1‑91 (MQ=255)

                                                                   tAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAttt                         >  1:752472/1‑91 (MQ=255)

                                                                    aaGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtg                        >  1:3775634/1‑91 (MQ=255)

                                                                    aaGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtg                        >  1:3435269/1‑91 (MQ=255)

                                                                     aGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgt                       >  1:754595/1‑91 (MQ=255)

                                                                      ggTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgtg                      >  1:2088869/1‑91 (MQ=255)

                                                                      ggTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgtg                      >  1:3258467/1‑91 (MQ=255)

                                                                         gtTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGt                   >  1:1677385/1‑91 (MQ=255)

                                                                          ttAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGtt                  >  1:143746/1‑91 (MQ=255)

                                                                           tAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTa                 >  1:2959782/1‑91 (MQ=255)

                                                                               aaaGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGata             <  1:1341895/91‑1 (MQ=255)

                                                                                 aGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATAtt           >  1:3619146/1‑91 (MQ=255)

                                                                                 aGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATAtt           >  1:1816709/1‑91 (MQ=255)

                                                                                 aGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATAtt           >  1:3155138/1‑91 (MQ=255)

                                                                                  gTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTg          <  1:2815641/91‑1 (MQ=255)

                                                                                   tATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg         <  1:3039010/91‑1 (MQ=255)

                                                                                   tATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg         <  1:4089488/91‑1 (MQ=255)

                                                                                   tATTGTCAATTATCGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg         >  1:3514033/1‑91 (MQ=255)

                                                                                   tATTGTCAATTATCGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg         >  1:2383748/1‑91 (MQ=255)

                                                                                    aTTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGt        <  1:376750/91‑1 (MQ=255)

                                                                                        tCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCaa    <  1:3045535/91‑1 (MQ=255)

                                                                                          gATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATg  >  1:2379757/1‑91 (MQ=255)

TCCGCCAATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATG  >  USA300TCH1516_ALE/413668‑413848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`