BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	413,764	T→C	5.7%	S165S (TCA→TCG)	metI ←	Cystathionine gamma‑synthase/O‑acetylhomoserine (thiol)‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	413,764	0	T	C	5.7%	340.0 / 8.4	105	S165S (TCA→TCG)	metI	Cystathionine gamma‑synthase/O‑acetylhomoserine (thiol)‑lyase
Reads supporting (aligned to +/- strand): ref base T (51/48); new base C (2/4); total (53/52)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 108 total aligned reads displayed.

ATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGAATAA  >  USA300TCH1516_ALE/413675‑413853

atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                          >  1:1134217/1‑91 (MQ=255)

atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                          <  1:2889159/91‑1 (MQ=255)

atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                          <  1:2461929/91‑1 (MQ=255)

atatatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                          <  1:3766451/91‑1 (MQ=255)

atatatTTCGTCGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTg                                                                                          <  1:3766364/91‑1 (MQ=255)

  atatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGAc                                                                                        >  1:874519/1‑91 (MQ=255)

  atatTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGAc                                                                                        <  1:681386/91‑1 (MQ=255)

    atTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACaa                                                                                      <  1:3149669/91‑1 (MQ=255)

     tttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAAt                                                                                     >  1:1460896/1‑91 (MQ=255)

      ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATa                                                                                    >  1:3831659/1‑91 (MQ=255)

      ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                                    <  1:2747204/91‑1 (MQ=255)

      ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                                    <  1:585910/91‑1 (MQ=255)

      ttCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATa                                                                                    <  1:2042469/91‑1 (MQ=255)

       tCGTGGCTGAATGCAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCAATTATCGACAATac                                                                                   <  1:4142771/91‑2 (MQ=255)

          tGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGcc                                                                                <  1:3918744/91‑1 (MQ=255)

          tGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGcc                                                                                <  1:3918687/91‑1 (MQ=255)

            gCTGAATGTAAGACTTTATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTACAAAAGTATTGTCGATAATTGACAATAAGCCtt                                                                              >  1:3536478/1‑91 (MQ=255)

            gCTGAATGTAAGACTGTATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCtt                                                                              >  1:3537020/1‑91 (MQ=255)

             cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGccctt                                                                             >  1:3823592/1‑88 (MQ=255)

             cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCttt                                                                             <  1:2498963/91‑1 (MQ=255)

             cTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCttt                                                                             >  1:13316/1‑91 (MQ=255)

               gAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAg                                                                           >  1:743187/1‑91 (MQ=255)

                 aTGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCt                                                                         <  1:3662885/91‑1 (MQ=255)

                  tGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCtt                                                                        >  1:3490650/1‑91 (MQ=255)

                  tGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCtt                                                                        >  1:3924072/1‑91 (MQ=255)

                   gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                       >  1:3437152/1‑91 (MQ=255)

                   gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                       >  1:3437865/1‑91 (MQ=255)

                   gTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCttt                                                                       >  1:402064/1‑91 (MQ=255)

                    tAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTa                                                                      <  1:2456585/91‑1 (MQ=255)

                     aaGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTAc                                                                     <  1:2715058/91‑1 (MQ=255)

                         cTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAg                                                                 <  1:2165246/91‑1 (MQ=255)

                         cTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAg                                                                 >  1:4123777/1‑91 (MQ=255)

                          tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                                <  1:2867404/91‑1 (MQ=255)

                          tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                                >  1:2590585/1‑91 (MQ=255)

                          tataTCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGt                                                                <  1:2867002/91‑1 (MQ=255)

                              tCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGAt                                                            <  1:2247450/91‑1 (MQ=255)

                                aGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGTTAg                                                          >  1:3350696/1‑91 (MQ=255)

                                 gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                         >  1:3637550/1‑91 (MQ=255)

                                 gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                         <  1:1464206/91‑1 (MQ=255)

                                 gCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGt                                                         >  1:3773358/1‑91 (MQ=255)

                                  cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                        <  1:830772/91‑1 (MQ=255)

                                  cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                        <  1:830187/91‑1 (MQ=255)

                                  cACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTa                                                        >  1:3063053/1‑91 (MQ=255)

                                   aCCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTAt                                                       <  1:2259020/91‑1 (MQ=255)

                                    ccTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATg                                                      <  1:2802422/91‑1 (MQ=255)

                                     cTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATgg                                                     <  1:253434/91‑1 (MQ=255)

                                      ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                                    <  1:484259/91‑1 (MQ=255)

                                      ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                                    >  1:1167502/1‑91 (MQ=255)

                                      ttCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGc                                                    <  1:1422241/91‑1 (MQ=255)

                                       tCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCt                                                   <  1:848562/91‑1 (MQ=255)

                                        cTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCtt                                                  >  1:4006402/1‑91 (MQ=255)

                                        cTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCtt                                                  <  1:3805656/91‑1 (MQ=255)

                                         tGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCttt                                                 >  1:135531/1‑91 (MQ=255)

                                         tGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCttt                                                 >  1:3703452/1‑91 (MQ=255)

                                          gCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTa                                                <  1:3392903/91‑1 (MQ=255)

                                          gCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTa                                                <  1:229633/91‑1 (MQ=255)

                                           cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                               <  1:570113/91‑1 (MQ=255)

                                           cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                               >  1:3504245/1‑91 (MQ=255)

                                           cTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTAc                                               >  1:2573576/1‑91 (MQ=255)

                                             aGTGGTGCTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACAt                                             <  1:2082145/91‑1 (MQ=255)

                                              gtggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATc                                            <  1:1696307/91‑1 (MQ=255)

                                               tggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCa                                           >  1:1900904/1‑91 (MQ=255)

                                               tggtgTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCa                                           >  1:3151102/1‑91 (MQ=255)

                                                  tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                        <  1:260371/91‑1 (MQ=255)

                                                  tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                        <  1:261798/91‑1 (MQ=255)

                                                  tgtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATa                                        <  1:262060/91‑1 (MQ=255)

                                                   gtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAg                                       <  1:2231924/91‑1 (MQ=255)

                                                   gtTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAg                                       <  1:823707/91‑1 (MQ=255)

                                                     tGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCa                                     >  1:2954736/1‑91 (MQ=255)

                                                     tGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCa                                     >  1:1851572/1‑91 (MQ=255)

                                                      gAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCaa                                    <  1:3234485/91‑1 (MQ=255)

                                                       aaaGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAAt                                   >  1:3673359/1‑91 (MQ=255)

                                                          gATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAt                                >  1:1208886/1‑91 (MQ=255)

                                                           aTAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAtt                               <  1:1273626/91‑1 (MQ=255)

                                                            tAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAttt                              >  1:752472/1‑91 (MQ=255)

                                                            tAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCAttt                              >  1:752718/1‑91 (MQ=255)

                                                             aaGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtg                             >  1:3775634/1‑91 (MQ=255)

                                                             aaGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtg                             >  1:3435269/1‑91 (MQ=255)

                                                              aGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgt                            >  1:754595/1‑91 (MQ=255)

                                                               ggTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgtg                           >  1:2088869/1‑91 (MQ=255)

                                                               ggTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTtgtg                           >  1:3258467/1‑91 (MQ=255)

                                                                  gtTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGt                        >  1:1678000/1‑91 (MQ=255)

                                                                  gtTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGt                        >  1:1677385/1‑91 (MQ=255)

                                                                   ttAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGtt                       >  1:143746/1‑91 (MQ=255)

                                                                    tAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTa                      >  1:2959782/1‑91 (MQ=255)

                                                                        aaaGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGata                  <  1:1341895/91‑1 (MQ=255)

                                                                          aGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATAtt                >  1:3155138/1‑91 (MQ=255)

                                                                          aGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATAtt                >  1:1816709/1‑91 (MQ=255)

                                                                            tATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg              <  1:4089488/91‑1 (MQ=255)

                                                                            tATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg              <  1:3039010/91‑1 (MQ=255)

                                                                            tATTGTCAATTATCGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg              >  1:2383748/1‑91 (MQ=255)

                                                                            tATTGTCAATTATCGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTgg              >  1:3514033/1‑91 (MQ=255)

                                                                             aTTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGt             <  1:376750/91‑1 (MQ=255)

                                                                                 tCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCaa         <  1:3045535/91‑1 (MQ=255)

                                                                                    aTAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGa      <  1:3030691/91‑1 (MQ=255)

                                                                                       aTTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGaata   >  1:3445239/1‑91 (MQ=255)

                                                                                       aTTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGaata   >  1:4032123/1‑91 (MQ=255)

                                                                                        ttGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGaataa  >  1:594209/1‑91 (MQ=255)

                                                                                        ttGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGaataa  >  1:4120829/1‑91 (MQ=255)

                                                                                        ttGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGaataa  >  1:4120448/1‑91 (MQ=255)

ATATATTTCGTGGCTGAATGTAAGACTATATCAGCACCTTCTGCTAGTGGTGTTGAAAGATAAGGTGTTAAAAAAGTATTGTCGATAATTGACAATAAGCCTTTAGCTTTACAAAGTTGATAGTATGGCTTTACATCAATAGCAATCATTTGTGGGTTAGATATTGGTTCAATGAATAA  >  USA300TCH1516_ALE/413675‑413853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`