BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,362	G→A	H104Y (CAC→TAC)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,362	0	G	A	100.0%	435.3 / NA	117	H104Y (CAC→TAC)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (52/65); total (52/65)

Only 100 of 117 total aligned reads displayed.

AATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTG  >  USA300TCH1516_ALE/1744274‑1744448

  ttttATGGCTGCTTCAATCGTACCACCAGTAGCTAATAAAGCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACAtt                                                                                    <  1:1024591/91‑1 (MQ=255)

    ttATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGt                                                                                  <  1:1999354/91‑1 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 >  1:1896465/1‑91 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 >  1:354336/1‑91 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 >  1:2817203/1‑91 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 <  1:1993908/91‑1 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 >  1:2578150/1‑91 (MQ=255)

     tATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGtt                                                                                 <  1:1188358/91‑1 (MQ=255)

       ttGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaa                                                                               >  1:2687378/1‑91 (MQ=255)

        tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaaa                                                                              >  1:561771/1‑91 (MQ=255)

        tgctgcTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaaa                                                                              <  1:3678954/91‑1 (MQ=255)

         gctgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTaaaa                                                                             <  1:1287912/91‑1 (MQ=255)

          ctgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAAc                                                                            >  1:2570887/1‑91 (MQ=255)

           tgctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACa                                                                           >  1:666291/1‑91 (MQ=255)

            gctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAt                                                                          >  1:1269987/1‑91 (MQ=255)

            gctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAt                                                                          <  1:460163/91‑1 (MQ=255)

             ctTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAtt                                                                         >  1:3298347/1‑91 (MQ=255)

              ttCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAttt                                                                        <  1:1569794/91‑1 (MQ=255)

              ttCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACAttt                                                                        <  1:859730/91‑1 (MQ=255)

                cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                      <  1:1983433/91‑1 (MQ=255)

                cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                      <  1:1981909/91‑1 (MQ=255)

                cAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGt                                                                      >  1:2328048/1‑91 (MQ=255)

                  aTCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAc                                                                    >  1:1722027/1‑91 (MQ=255)

                   tCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTAcc                                                                   <  1:250630/91‑1 (MQ=255)

                    cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCa                                                                  <  1:3266605/91‑1 (MQ=255)

                    cGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCa                                                                  <  1:1558717/91‑1 (MQ=255)

                      tACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCata                                                                >  1:1238893/1‑91 (MQ=255)

                         caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTc                                                             >  1:2210404/1‑91 (MQ=255)

                         caccaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTc                                                             <  1:794096/91‑1 (MQ=255)

                          accaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                            >  1:3270061/1‑91 (MQ=255)

                          accaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                            >  1:1934840/1‑91 (MQ=255)

                          accaGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCt                                                            >  1:2179199/1‑91 (MQ=255)

                             aGTAGCTAATAAATCATCTGTAATTAACACACATTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAgg                                                         <  1:3152215/91‑1 (MQ=255)

                              gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGt                                                        <  1:2364146/91‑1 (MQ=255)

                              gTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGt                                                        <  1:2364093/91‑1 (MQ=255)

                               tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTc                                                       <  1:452107/91‑1 (MQ=255)

                               tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTc                                                       >  1:1535580/1‑91 (MQ=255)

                               tAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTc                                                       <  1:452303/91‑1 (MQ=255)

                                aGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCa                                                      <  1:213419/91‑1 (MQ=255)

                                 gCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCAt                                                     <  1:3195860/91‑1 (MQ=255)

                                 gCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCAt                                                     >  1:1494217/1‑91 (MQ=255)

                                 gCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCAt                                                     <  1:3081456/91‑1 (MQ=255)

                                  cTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATACTCTAGGttcga                                                    >  1:1062500/1‑87 (MQ=255)

                                    aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACt                                                  <  1:3623005/91‑1 (MQ=255)

                                    aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACt                                                  >  1:298846/1‑91 (MQ=255)

                                    aataaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACt                                                  >  1:2578542/1‑91 (MQ=255)

                                      taaATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCa                                                <  1:1081824/91‑1 (MQ=255)

                                       anaTCATCTNTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTATACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCAt                                               <  1:2181273/91‑1 (MQ=255)

                                       aaaTCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCAt                                               >  1:2214198/1‑91 (MQ=255)

                                           catcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACg                                           <  1:3657987/91‑1 (MQ=255)

                                           catcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACg                                           >  1:3238389/1‑91 (MQ=255)

                                            atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGa                                          <  1:314342/91‑1 (MQ=255)

                                            atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGa                                          >  1:2673471/1‑91 (MQ=255)

                                             tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAGCATTTGTACCATATTCTAGGTCATACTCATAACGaa                                         <  1:2672003/91‑1 (MQ=255)

                                             tcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGaa                                         >  1:3105735/1‑91 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATTTTCTAGGTCATACTCATAACGAAt                                        >  1:2401551/1‑91 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAt                                        >  1:1704232/1‑91 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAt                                        <  1:629269/91‑1 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAt                                        >  1:1751659/1‑91 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAAt                                        >  1:2401882/1‑91 (MQ=255)

                                              cTGTAATTAACACACGTTGACCTGGTTTAATCGCATCTTTGTACGTTGTTAAAGCATGTGTACCATATTCTAGGTCATACTCATAACGAAt                                        <  1:2952302/91‑1 (MQ=255)

                                                gTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGa                                      >  1:1651934/1‑91 (MQ=255)

                                                 tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCGTAACGAATGAc                                     >  1:3359648/1‑91 (MQ=255)

                                                   aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACtt                                   >  1:1821514/1‑91 (MQ=255)

                                                   aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACtt                                   >  1:171223/1‑91 (MQ=255)

                                                     tAACCCACGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCTTATTCTAGGTCATACTCATAACGAATGACCTCa                                 >  1:2922209/1‑91 (MQ=255)

                                                       acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACg                               <  1:3487141/91‑1 (MQ=255)

                                                       acacacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACg                               >  1:1662333/1‑91 (MQ=255)

                                                         acacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAg                             <  1:276450/91‑1 (MQ=255)

                                                          cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                            <  1:1549310/91‑1 (MQ=255)

                                                          cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                            <  1:3336828/91‑1 (MQ=255)

                                                          cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                            >  1:608617/1‑91 (MQ=255)

                                                          cacGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAgg                            >  1:863661/1‑91 (MQ=255)

                                                            cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTa                          >  1:3090337/1‑91 (MQ=255)

                                                            cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTa                          <  1:1541294/91‑1 (MQ=255)

                                                            cGTTGACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTa                          >  1:2566737/1‑91 (MQ=255)

                                                                gACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                      <  1:2667946/91‑1 (MQ=255)

                                                                gACCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAAttt                      <  1:2667769/91‑1 (MQ=255)

                                                                 aCCTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTc                     <  1:3006732/91‑1 (MQ=255)

                                                                  ccTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                    >  1:1206089/1‑91 (MQ=255)

                                                                  ccTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                    <  1:366292/91‑1 (MQ=255)

                                                                  ccTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcc                    <  1:2284010/91‑1 (MQ=255)

                                                                   cTGGTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTccc                   <  1:1800800/91‑1 (MQ=255)

                                                                     ggTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTcccgt                 <  1:1231411/91‑3 (MQ=255)

                                                                      gTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCcttc                >  1:2548792/1‑91 (MQ=255)

                                                                      gTTTAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCcttc                <  1:653500/91‑1 (MQ=255)

                                                                         tAATTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCttt             <  1:2126677/91‑1 (MQ=255)

                                                                          aaTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt            <  1:2170950/91‑1 (MQ=255)

                                                                          aaTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt            <  1:3111282/91‑1 (MQ=255)

                                                                          aaTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCtttt            >  1:1388400/1‑91 (MQ=255)

                                                                           aTTGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTc           <  1:2699136/91‑1 (MQ=255)

                                                                             tGCATCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTa         <  1:1249992/91‑1 (MQ=255)

                                                                                aTCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACa      <  1:526448/91‑1 (MQ=255)

                                                                                 tCTTTGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAg     <  1:2035408/91‑1 (MQ=255)

                                                                                   tttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGt   >  1:880468/1‑91 (MQ=255)

                                                                                   tttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGt   >  1:880546/1‑91 (MQ=255)

                                                                                   tttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGt   <  1:2750906/91‑1 (MQ=255)

                                                                                   tttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGt   >  1:1987249/1‑91 (MQ=255)

                                                                                    ttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTg  >  1:137496/1‑91 (MQ=255)

                                                                                    ttGTACATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTg  <  1:2966044/91‑1 (MQ=255)

AATTTTATTGCTGCTTCAATCGTACCACCAGTAGCTAATAAATCATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTG  >  USA300TCH1516_ALE/1744274‑1744448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`