Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,470,111 G→A 50.0% S575L (TCA→TTA)  emrB ← Multidrug export protein EmrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,470,1110GA50.0% ‑4.0 / 34.5 26S575L (TCA→TTA) emrBMultidrug export protein EmrB
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base A (3/10);  total (9/17)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAATTAAGGCGATGATGCTAAATATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTACCTATAGAGCCTGCTAAT  >  USA300TCH1516_ALE/2469984‑2470245
                                                                                                                               |                                                                                                                                      
tAAAATTAAGGCGATGATGCTAAATATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATg                                                                                                                           <  1:554914/141‑1 (MQ=255)
      tAAGTCGATGATGCTAAATATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATg                                                                                                                     <  1:29924/141‑1 (MQ=255)
         ggCGATGATGCTAAATATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATc                                                                                                                  >  1:67189/1‑141 (MQ=255)
                    tAAATATCGTTGTAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAATTTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATAGTGATGCTAATTCATGCATATGATCTTGTACAACAg                                                                                                       <  1:397480/141‑1 (MQ=255)
                     aaaTATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAgg                                                                                                      <  1:238780/141‑1 (MQ=255)
                                           tcatcatTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTcc                                                                                <  1:519237/140‑1 (MQ=255)
                                           gCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGACTTCTTGTCCGCCATATTGTAATGCTAATTCATGTATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTcc                                                                                <  1:387111/141‑1 (MQ=255)
                                                  tAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAg                                                                         >  1:483282/1‑141 (MQ=255)
                                                    aTACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCt                                                                       <  1:509528/141‑1 (MQ=255)
                                                     tACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAATACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTg                                                                      <  1:27052/141‑1 (MQ=255)
                                                                 ttGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTtgtg                                                          >  1:209224/1‑141 (MQ=255)
                                                                        tttatttaCAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAATAGCTGATAAGTGTTGTGTTGTTtg                                                   >  1:355532/1‑141 (MQ=255)
                                                                          tatttaCAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTtgtg                                                 <  1:53616/141‑1 (MQ=255)
                                                                                 aaaTTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCAt                                          >  1:499249/1‑141 (MQ=255)
                                                                                          taACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCCTTACAGTagc                                 >  1:222093/1‑139 (MQ=255)
                                                                                               cTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGa                            >  1:176788/1‑141 (MQ=255)
                                                                                               cTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGa                            <  1:161455/141‑1 (MQ=255)
                                                                                               cTTTCATTGCGCCTTCTTGGCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGa                            <  1:193472/141‑1 (MQ=255)
                                                                                                tttCATTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAACGCGGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGAt                           <  1:325410/141‑1 (MQ=255)
                                                                                                    aTTGCGCCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGGTAAGTGTTGTGTAGTTTGTGTTGTCATTAGAGTAACTAAGATTGCt                       >  1:485118/1‑141 (MQ=255)
                                                                                                      tGCCCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTGCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGt                     <  1:429523/141‑1 (MQ=255)
                                                                                                       gcgcCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTa                    >  1:570942/1‑141 (MQ=255)
                                                                                                         gcCTTCTTGTCCGCCATATTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTAcc                  <  1:370380/141‑1 (MQ=255)
                                                                                                            ttcttGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTACCtat               <  1:67773/141‑1 (MQ=255)
                                                                                                                         tatTGTAATGCTAATTGCTGCATTTGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTACCTATAGAGCCTGCTAAt  <  1:416357/141‑1 (MQ=255)
                                                                                                                         tatTGTAATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTTTTCTCATTACAGTAACTAAGATTGCTGTACCTATAGAGCCTGCTAAt  <  1:416515/141‑1 (MQ=255)
                                                                                                                               |                                                                                                                                      
TAAAATTAAGGCGATGATGCTAAATATCGTTGCAACTATAAATGCATCATTAATACCTTCAACCGTTGCTAGTTTATTTACAAATTGTAGTAACACTTTCATTGCGCCTTCTTGTCCGCCATATTGTGATGCTAATTCATGCATATGATCTTGTACAACAGGATTCGTTTTATCTAACTCTTCCCCAAAAGCTGATAAGTGTTGTGTAGTTTGTGTTGTCATTACAGTAACTAAGATTGCTGTACCTATAGAGCCTGCTAAT  >  USA300TCH1516_ALE/2469984‑2470245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: