Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 665,392 T→G 100% L79* (TTA→TGA)  paiA_1 → Spermidine/spermine N(1)‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE665,3920TG100.0% 125.7 / NA 39L79* (TTA→TGA) paiA_1Spermidine/spermine N(1)‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/18);  total (21/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGA  >  USA300TCH1516_ALE/665257‑665527
                                                                                                                                       |                                                                                                                                       
caacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTCTATTTACGGTGAAGAATGatat                                                                                                                                     >  1:711320/1‑140 (MQ=255)
 aacaGTTGATCACTTGTTAGAAGCATCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTCACGGTGAAGAATGATAtt                                                                                                                                    >  1:486681/1‑140 (MQ=255)
 aacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtt                                                                                                                                    >  1:52189/1‑140 (MQ=255)
              ttGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACAGCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTa                                                                                                                       <  1:347076/140‑1 (MQ=255)
                  tAGAAGCTTCATATAATGAAGATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTGACTTTATTTACGGTGAAGAATGATATTTATCAGCCCATTCTGtt                                                                                                                   >  1:537414/1‑140 (MQ=255)
                    gAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTaa                                                                                                                 <  1:683718/140‑1 (MQ=255)
                     aaGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTaaa                                                                                                                >  1:765207/1‑140 (MQ=255)
                      agcatactataATGATAATCATTTAAAGAAAAAACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAc                                                                                                               <  1:132669/133‑1 (MQ=255)
                      aGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAc                                                                                                               <  1:577671/140‑1 (MQ=255)
                         ttCATATAATGAATATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAg                                                                                                            <  1:381398/140‑1 (MQ=255)
                             tataATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                        >  1:428729/1‑140 (MQ=255)
                             tataATGAACATCACTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAGTGATATTTATCAGCTCATTATGTTAAACCAGAATc                                                                                                        >  1:423715/1‑140 (MQ=255)
                               taATGAACATCATTTAAATAAAAGACTTCAAGAACAATTATTCCTAGTCGGTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGc                                                                                                      <  1:155920/140‑1 (MQ=255)
                                    aaCATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGCTATTTATCAGCTCATTATGTTAAACCAGAATCGCAACAt                                                                                                 >  1:541325/1‑140 (MQ=255)
                                         catTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                            <  1:508552/140‑1 (MQ=255)
                                         catTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                            <  1:733501/140‑1 (MQ=255)
                                         catTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAAGGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAgg                                                                                            <  1:576340/140‑1 (MQ=255)
                                          atTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGt                                                                                           <  1:139410/140‑1 (MQ=255)
                                            ttAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTa                                                                                         >  1:373082/1‑140 (MQ=255)
                                                         ttCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTg                                                                            <  1:703719/140‑1 (MQ=255)
                                                          tCAAGAACAACTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTTAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTGATGGTACAGCATTGt                                                                           <  1:570339/140‑1 (MQ=255)
                                                           cAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGATAAACCAGAATAGCAACATACAGGTTATGGTACAGCATTGtt                                                                          >  1:99988/1‑140 (MQ=255)
                                                            aaGAACAATTATTCTTAGTCGTTGAAGAAGGGAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCGCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTa                                                                         <  1:98144/140‑1 (MQ=255)
                                                              gAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTGACTTTGTTTACGGTGACGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCANCATATAGGTGATGGTANAGCATTGTTaca                                                                       >  1:222397/1‑138 (MQ=255)
                                                                      atccTTAGTCGTTTAAGAAGGTAATGACATCGTTGACTTTGCTAACTTTATTTACAGTGAAGAATGATATATAACAGCTCATTATGTTAAACAAGACTCTCAACATACAGGTTATGGTACAGCATTGTTAAATGAAgaat                                                               <  1:25778/137‑4 (MQ=255)
                                                                           tAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCa                                                          <  1:7621/140‑1 (MQ=255)
                                                                              tCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGt                                                       >  1:280744/1‑140 (MQ=255)
                                                                                gTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGttt                                                     >  1:219423/1‑140 (MQ=255)
                                                                                gTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGttt                                                     >  1:280072/1‑140 (MQ=255)
                                                                                      gaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGa                                               <  1:613981/140‑1 (MQ=255)
                                                                                      gaagGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGGGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGa                                               >  1:524109/1‑140 (MQ=255)
                                                                                                aCATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                                     >  1:343190/1‑140 (MQ=255)
                                                                                                       tGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATAACGTTTTGAAGATAAATTTGAAGGTGttt                              >  1:159639/1‑140 (MQ=255)
                                                                                                       tGGCTTTACTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGttt                              >  1:689190/1‑140 (MQ=255)
                                                                                                       tGGCTTTACTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGCAATGGTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGgtt                              >  1:331188/1‑140 (MQ=255)
                                                                                                          ttttGCTAACTTTATTTACGGTGAATAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACt                           <  1:269016/139‑1 (MQ=255)
                                                                                                                 aaCTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGt                    <  1:177989/140‑1 (MQ=255)
                                                                                                                                 aagaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATgaa    >  1:366083/1‑140 (MQ=255)
                                                                                                                                   gaaTGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAACgaaga  >  1:709649/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAATGAAGA  >  USA300TCH1516_ALE/665257‑665527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: