Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,544,339	C→T	V189V (GTC→GTT)	hlgC_2 →	Gamma‑hemolysin component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,544,339	0	C	T	100.0%	60.9 / NA	17	V189V (GTC→GTT)	hlgC_2	Gamma‑hemolysin component C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/5);  total (12/5)

GGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTG  >  USA300TCH1516_ALE/2544193‑2544478                                                                                                                                                   |                                                                                                                                            ggATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTaa                                                                                                                                           >  1:196851/1‑149 (MQ=255) ggATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTaa                                                                                                                                           >  1:210/1‑149 (MQ=255)                         cAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACGATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATc                                                                                                                   <  1:252437/149‑1 (MQ=255)                                       aCTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAg                                                                                                     <  1:80396/149‑1 (MQ=255)                                            gtggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCttt                                                                                                >  1:326697/1‑149 (MQ=255)                                                                        aTCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAAc                                                                    >  1:34350/1‑149 (MQ=255)                                                                              tAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATa                                                              <  1:296283/149‑1 (MQ=255)                                                                                    taCACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAg                                                        >  1:65341/1‑149 (MQ=255)                                                                                       acaacaAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATc                                                     >  1:83657/1‑149 (MQ=255)                                                                                                       aGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGt                                     >  1:58696/1‑149 (MQ=255)                                                                                                         tGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGCCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTc                                   <  1:176832/149‑1 (MQ=255)                                                                                                           aaGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCa                                 >  1:14272/1‑149 (MQ=255)                                                                                                             gTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGa                               >  1:108175/1‑149 (MQ=255)                                                                                                              tAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGAc                              >  1:215035/1‑149 (MQ=255)                                                                                                                 aacaacAAAACTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGCTTTATTTGTAGGATCCACAGCTCATGGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGt                           >  1:220500/1‑149 (MQ=255)                                                                                                                          aCTCAAAAAGTGTTTTATGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTacca                  <  1:91371/149‑1 (MQ=255)                                                                                                                                          aTGGGGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTg  >  1:126876/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                            GGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGGGGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCTCATAGTAAAGATCCTAGAGATTATTTCGTTCCAGACAGTGAGTTACCACCTCTTGTACAAAGTG  >  USA300TCH1516_ALE/2544193‑2544478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑