Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,709,576	C→T	G451S (GGT→AGT)	oatA_2 ←	O‑acetyltransferase OatA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,709,576	0	C	T	100.0%	45.4 / NA	13	G451S (GGT→AGT)	oatA_2	O‑acetyltransferase OatA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

TTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGT  >  USA300TCH1516_ALE/2709432‑2709715                                                                                                                                                 |                                                                                                                                            tttGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAAtt                                                                                                                                         <  1:214807/149‑1 (MQ=255)         tttttAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAgtgg                                                                                                                                 >  1:248249/1‑149 (MQ=255)                             ttGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCct                                                                                                             <  1:339254/149‑1 (MQ=255)                                 gATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCttt                                                                                                         >  1:72683/1‑149 (MQ=255)                                                                    tCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCtt                                                                      >  1:10184/1‑149 (MQ=255)                                                                            tACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACg                                                              >  1:314264/1‑149 (MQ=255)                                                                                  cAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACtttt                                                        >  1:339784/1‑149 (MQ=255)                                                                                                              aaaGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttct                            <  1:35315/149‑1 (MQ=255)                                                                                                                aGACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttcttc                          <  1:290286/149‑1 (MQ=255)                                                                                                                 gACATTACCAATATCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtcttcttct                         >  1:91924/1‑149 (MQ=255)                                                                                                                              tCCACCATGACCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGc            <  1:134942/149‑1 (MQ=255)                                                                                                                                       aCCGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg   >  1:162998/1‑149 (MQ=255)                                                                                                                                        ccGAGTCACTAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttgt  <  1:9770/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                            TTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGT  >  USA300TCH1516_ALE/2709432‑2709715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑