Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,761,889 C→A 100% D388Y (GAT→TAT)  fpgS ← Folylpolyglutamate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,761,8890CA100.0% 102.6 / NA 33D388Y (GAT→TAT) fpgSFolylpolyglutamate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/16);  total (17/16)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAAT  >  USA300TCH1516_ALE/1761753‑1762018
                                                                                                                                        |                                                                                                                                 
tcttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATatact                                                                                                                               <  1:612245/141‑1 (MQ=255)
  ttcATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTaa                                                                                                                             >  1:214569/1‑141 (MQ=255)
   tcATGGCTATTTTAGTTTTGGTTTTATAATGCTTCAAAGTCTAAGTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAAt                                                                                                                            >  1:447176/1‑141 (MQ=255)
     aTGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTg                                                                                                                          <  1:96680/141‑1 (MQ=255)
      tGGCTATTTTAGTTTTAGTATTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAACTACAGACCTCCGGTAATGACTAAGGTATCACGTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGt                                                                                                                         >  1:435499/1‑141 (MQ=255)
       ggCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGtt                                                                                                                        >  1:225744/1‑140 (MQ=255)
        gCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACGGGTAAGTACTAATGTATCACTGTGATATTTTTTTATAAATTCAGCGTAGTCATATACTAATTGTAt                                                                                                                       >  1:169181/1‑141 (MQ=255)
              ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCCACGTAGTCATACACTAATTGTATTtcatc                                                                                                                 >  1:767289/1‑141 (MQ=255)
              ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatc                                                                                                                 >  1:123372/1‑141 (MQ=255)
              ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatc                                                                                                                 >  1:374050/1‑141 (MQ=255)
              ttagttttagttTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTtcatc                                                                                                                 >  1:137020/1‑141 (MQ=255)
                  ttttagttttaTAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCCTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCAttt                                                                                                             <  1:541726/141‑1 (MQ=255)
                             taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacc                                                                                                  <  1:705708/141‑1 (MQ=255)
                             taATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATActacc                                                                                                  <  1:532588/141‑1 (MQ=255)
                                            tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAGATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTTCCTACAATTTCTTCttt                                                                                   >  1:409498/1‑141 (MQ=255)
                                            tAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCttt                                                                                   >  1:236743/1‑141 (MQ=255)
                                                tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                               <  1:178188/141‑1 (MQ=255)
                                                tttGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGt                                                                               <  1:509952/141‑1 (MQ=255)
                                                      ttAACTGCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCt                                                                         <  1:305699/141‑1 (MQ=255)
                                                              aCTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTGTCGGaaa                                                                 >  1:80217/1‑141 (MQ=255)
                                                                 tATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCATTACTGCCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATc                                                              >  1:681620/1‑141 (MQ=255)
                                                                             aCTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGc                                                  <  1:241312/141‑1 (MQ=255)
                                                                               tACCGGTAATTACTAATGTATCACCTGGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCTATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCAt                                                <  1:497993/141‑1 (MQ=255)
                                                                                     tAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACg                                          <  1:809497/141‑1 (MQ=255)
                                                                                         tACTAAGGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATg                                      <  1:142591/141‑1 (MQ=255)
                                                                                                   tCACCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGAGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTaaa                            <  1:26788/141‑1 (MQ=255)
                                                                                                     aCCTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTCCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACt                          <  1:228152/141‑1 (MQ=255)
                                                                                                       cTTGATAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATCATACGGATGCGCAATTAAACTTa                        <  1:806431/141‑1 (MQ=255)
                                                                                                           aTAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATg                    >  1:23908/1‑141 (MQ=255)
                                                                                                            tAATTTTTTATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGt                   >  1:327344/1‑141 (MQ=255)
                                                                                                                tttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa               >  1:118109/1‑141 (MQ=255)
                                                                                                                tttttATAAATTCAACGTACTCATACACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGa               >  1:449006/1‑141 (MQ=255)
                                                                                                                 ttttATAAATTCAACGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGAc              <  1:709096/141‑1 (MQ=255)
                                                                                                                             aaCGTAGTCATATACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCCATTGTCAGTAAATGTTTGACCATCTCGTTAAt  <  1:396310/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TCTTCATGGCTATTTTAGTTTTAGTTTTATAATGCTTCAAAGTCTAATTTTGATTTAACTTCACTTATGAAATACAGACTACCGGTAATTACTAATGTATCACCTTGATAATTTTTTATAAATTCAACGTAGTCATCTACTAATTGTATTTCATCATTTTCAATACTACCTACAATTTCTTCTTTGCGTAACGCTTTCGGAAAATCAAATTCAGTTGCATAAAACGTATGCGCAATTAAACTTAAATGTTTGACCATCTCGTTAAT  >  USA300TCH1516_ALE/1761753‑1762018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑