Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 110,660 T→A 12.0% I173I (ATT→ATA)  USA300HOU_RS00515 → putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE110,6600TA12.0% 186.5 / 9.5 64I173I (ATT→ATA) USA300HOU_RS00515putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (30/26);  new base A (2/6);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  USA300TCH1516_ALE/110521‑110798
                                                                                                                                            |                                                                                                                                          
cGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGTCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATAttt                                                                                                                                           <  1:804848/141‑1 (MQ=255)
 ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAAGAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTc                                                                                                                                          <  1:286213/141‑1 (MQ=255)
  gTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCa                                                                                                                                         <  1:3740/141‑1 (MQ=255)
     aaaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCatat                                                                                                                                      <  1:783006/141‑1 (MQ=255)
      aaaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCAtata                                                                                                                                     >  1:372474/1‑141 (MQ=255)
                    aaaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAATAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATTCAGAGGTGcca                                                                                                                       >  1:78546/1‑140 (MQ=255)
                            tCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTc                                                                                                               <  1:135152/141‑1 (MQ=255)
                             ccAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGGGGTGCCGAGTTATTCg                                                                                                              <  1:405277/141‑1 (MQ=255)
                              caacaaAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTGAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                             >  1:550318/1‑141 (MQ=255)
                                     aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                      <  1:35410/141‑1 (MQ=255)
                                     aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAata                                                                                                      <  1:632180/141‑1 (MQ=255)
                                       aaaTTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTGCTGTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatata                                                                                                    >  1:697792/1‑140 (MQ=255)
                                           tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                >  1:278024/1‑141 (MQ=255)
                                           tAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAAtt                                                                                                >  1:500360/1‑141 (MQ=255)
                                           gaaaGATGAAAAAATAAAAAAAGAAATCGAAAACACTAAGTTCTTGGATCAATATGACGACATTAAAAAATGGAAAAAGTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCAGAGTTATAAGGCTAAATATCAAtt                                                                                                >  1:480818/2‑141 (MQ=17)
                                             aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                              >  1:577201/1‑141 (MQ=255)
                                             aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                              >  1:639762/1‑141 (MQ=255)
                                             aaGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                              >  1:603199/1‑141 (MQ=255)
                                              aGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTACCGAGTTATTCGGCTAAATATCAATTAAc                                                                                             >  1:368303/1‑141 (MQ=255)
                                               gATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATGTAATGAAGAGGTGCCGGGTTATTCGGCTAAATATGAATTaatt                                                                                            >  1:13240/1‑139 (MQ=17)
                                                           aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTACATATCAATTAACTAATGATGATtaa                                                                                >  1:41320/1‑140 (MQ=255)
                                                           aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                >  1:228997/1‑141 (MQ=255)
                                                           aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                <  1:411945/141‑1 (MQ=255)
                                                                      cGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                     >  1:6300/1‑141 (MQ=255)
                                                                      cGAAAACTTTAAGTTCTTTCTTCAATATGGCGACTTTAAAAATTTGACAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTacaaca                                                                     >  1:487995/1‑141 (MQ=255)
                                                                      cGAAAACTTCAAGTTCTGTTTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACa                                                                     <  1:644625/141‑1 (MQ=255)
                                                                        aacattttAAGTTCTTTGTTGAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                   <  1:95380/136‑1 (MQ=255)
                                                                        aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATCTAGAGGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAGGATGATTATAATGTAAAACAAt                                                                   <  1:388088/141‑1 (MQ=255)
                                                                        aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                                   <  1:251179/141‑1 (MQ=255)
                                                                             tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATTGCATCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                              <  1:595544/141‑1 (MQ=255)
                                                                              ttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTATCTCAAGTGAAGTAGAATGTAAAACAATTACGCa                                                             <  1:805693/141‑1 (MQ=17)
                                                                               tAAGTTCTTTGTTCAATATGGCGCCTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCTAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAACTACGCaa                                                            <  1:513665/141‑1 (MQ=255)
                                                                               tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                            <  1:315856/141‑1 (MQ=255)
                                                                               tAAGGTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                            <  1:787066/141‑1 (MQ=255)
                                                                                    tCTTTGTTCAATATTGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgat                                                       <  1:580832/141‑1 (MQ=255)
                                                                                          ttCAATATGGCGCATTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgatatgata                                                 <  1:56840/141‑1 (MQ=255)
                                                                                            cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCATGATGATTATAATGTAGAACAATTACGCAAAAGATATGAtgta                                               >  1:165707/1‑141 (MQ=255)
                                                                                            cAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTAAGCAAAAGATATGAtata                                               >  1:485106/1‑141 (MQ=255)
                                                                                                atGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAGCAATTACGCAAAAGATATGATATACcga                                           >  1:135790/1‑141 (MQ=255)
                                                                                                     gACTTTAAAAATTTGAAAAATTTTAAAGACGGA‑TATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGt                                      >  1:396661/1‑141 (MQ=255)
                                                                                                       cTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaa                                    <  1:645632/141‑1 (MQ=255)
                                                                                                         ttAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAg                                  >  1:533351/1‑141 (MQ=255)
                                                                                                           aaaaaTTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                                >  1:270059/1‑141 (MQ=255)
                                                                                                           aaaaaTTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                                >  1:378506/1‑141 (MQ=255)
                                                                                                           aaaaaTTTGAAAAATTATAAA‑ATGAATTAGATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                                >  1:195917/1‑141 (MQ=17)
                                                                                                                tttGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCaaa                           <  1:356056/141‑1 (MQ=255)
                                                                                                                   gAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGACTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGAAGAGTAAAGGTCCAAAGtt                        >  1:286032/1‑141 (MQ=255)
                                                                                                                    atgaaTGATAAAGAATGA‑GATATAACATAAAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                       <  1:603771/138‑1 (MQ=17)
                                                                                                                    aaaaaTTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                       <  1:32150/141‑1 (MQ=255)
                                                                                                                    aaaaaTTATAAAGACGGA‑GATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATGAACTAATGATCATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTa                       <  1:584258/141‑1 (MQ=255)
                                                                                                                        aTTATAAAGATGGA‑GATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt                   <  1:360717/141‑1 (MQ=255)
                                                                                                                        aTTATAAAGATGGA‑GATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt                   <  1:810814/141‑1 (MQ=255)
                                                                                                                            taAAGACGGA‑GATATTTCATACAATCCAGAGGTTCCGCGTTTCTGAAATCGCAATGGAGTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa               <  1:588702/141‑1 (MQ=18)
                                                                                                                             aaaGACGTA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa              <  1:397579/141‑1 (MQ=255)
                                                                                                                              aaGATGGA‑GATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAg             >  1:800053/1‑141 (MQ=255)
                                                                                                                              aaGATGGA‑GATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATGGTTAAAAg             >  1:472422/1‑141 (MQ=255)
                                                                                                                               aGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAgg            <  1:286864/141‑1 (MQ=255)
                                                                                                                                gatggA‑GATATATCATATAAGCCAGAGGTGCCGAGTGATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATCTACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt           <  1:454767/138‑1 (MQ=18)
                                                                                                                                 atggA‑GATATATCATATAATCCAGAGGTGCCGTGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGtt          <  1:504367/139‑1 (MQ=255)
                                                                                                                                     a‑gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      >  1:789392/1‑141 (MQ=255)
                                                                                                                                     a‑gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAgg      >  1:95676/1‑141 (MQ=255)
                                                                                                                                       gaTATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAggg     <  1:298085/141‑1 (MQ=255)
                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCGATGAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:158270/1‑141 (MQ=255)
                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:701627/1‑141 (MQ=255)
                                                                                                                                          atTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAt  >  1:674561/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
CGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGA‑GATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAAT  >  USA300TCH1516_ALE/110521‑110798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑