Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,279,705	G→A	A943V (GCA→GTA)	ebh_3 ←	Extracellular matrix‑binding protein ebh

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,279,705	0	G	A	100.0%	111.3 / NA	37	A943V (GCA→GTA)	ebh_3	Extracellular matrix‑binding protein ebh
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/20);  total (17/20)

GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  USA300TCH1516_ALE/2279567‑2279838                                                                                                                                           |                                                                                                                                      gTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt                                                                                                                                     >  1:63934/1‑141 (MQ=255)  ttGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                    >  1:342218/1‑141 (MQ=255)                gTTGATTCTACTTCTGGTTTACTAGTTACATAGTTATCCATTGTCGGACTGTTTGTTGATGCATCTAAACTAGAATTGTTATTAGCTTGTTTGTTATCATTTGCATCATCAATTGCTGATGTTACTGTTGTTTCACCTGtt                                                                                                                      >  1:162743/1‑141 (MQ=255)                     ttCTACTTCTGGTTTACTAGTTATATCGGTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGc                                                                                                                 <  1:161649/141‑1 (MQ=255)                      tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCa                                                                                                                <  1:204567/141‑1 (MQ=255)                        tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATc                                                                                                              >  1:442714/1‑141 (MQ=255)                         aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa                                                                                                             >  1:329918/1‑141 (MQ=255)                               ggTTTACTAGTTACATCGTCATCCATTGTCGGACTGTTTGTTGATGCCTCTACACTAGAATTGCTATTAGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtattt                                                                                                       >  1:266134/1‑140 (MQ=255)                                   tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg                                                                                                   <  1:56143/141‑1 (MQ=255)                                    aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGGTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTgg                                                                                                  <  1:335899/141‑1 (MQ=255)                                        gTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt                                                                                              <  1:179932/141‑1 (MQ=255)                                               cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag                                                                                       >  1:140363/1‑141 (MQ=255)                                                 ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTCTTATTTGGTGTTGTCggcggc                                                                                     >  1:178369/1‑137 (MQ=255)                                                   aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTACTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc                                                                                   <  1:339856/141‑1 (MQ=255)                                                     ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt                                                                                 >  1:441202/1‑141 (MQ=255)                                                          gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCCCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCt                                                                            >  1:150466/1‑141 (MQ=255)                                                             ggACCGTTTGTTGATGCATCTACACTAGAATTATTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGGTACTGTTGTCTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg                                                                         <  1:334565/141‑1 (MQ=255)                                                                cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                      <  1:308452/141‑1 (MQ=255)                                                                cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAATTGGTGTTGTCGGAGAAGCGTCTCCTTTGCCa                                                                      >  1:478142/1‑141 (MQ=255)                                                                   tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTa                                                                   >  1:192486/1‑141 (MQ=255)                                                                    ttgttgATGCATCTCCACTAGTATTGTTATTAGCTTGCGGTTTATCAGTTGCAGCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                                  <  1:419699/141‑1 (MQ=255)                                                                    ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACATGTTGCAGAATCAATCACATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg                                                                  >  1:224446/1‑141 (MQ=255)                                                                      gttgATGCACCTACACTAGAATTGGTATTAGCTTGCGGTTTATCATCTGCATCATCAGTTGCTGTGGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTCGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCt                                                                <  1:481251/141‑1 (MQ=255)                                                                           tGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                           <  1:181853/141‑1 (MQ=255)                                                                           tGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTCACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt                                                           <  1:337969/141‑1 (MQ=255)                                                                                      tAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt                                                <  1:443262/141‑1 (MQ=255)                                                                                       aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAACCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                               >  1:466564/1‑141 (MQ=255)                                                                                        gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTGAGATACGTTa                                              <  1:362908/141‑1 (MQ=255)                                                                                               tattaGCTTGCGGTTTATAATTTGCATCATCAGGTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                       <  1:83719/141‑1 (MQ=255)                                                                                                    gCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTa                                  <  1:315756/141‑1 (MQ=255)                                                                                                       cgCGGTTTATCATTTACATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTATCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTtttt                               <  1:359803/140‑1 (MQ=255)                                                                                                           gTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTCGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                           <  1:357203/141‑1 (MQ=255)                                                                                                                tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGtt                      >  1:438337/1‑141 (MQ=255)                                                                                                                tCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAGTTTCTGGTGtt                      >  1:438364/1‑141 (MQ=255)                                                                                                                     tGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAtt                 >  1:397803/1‑141 (MQ=255)                                                                                                                            tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGAAttt          <  1:339760/141‑1 (MQ=255)                                                                                                                                    tGATGTTACTGTTGTCTCACCTGTTGACGCATCAATATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCACATACGTTTGGTTGTCCAGTATTTTCTGGTGTCGCATTAGCATTTGAATTTGc  <  1:304754/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGC  >  USA300TCH1516_ALE/2279567‑2279838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑