| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 134,393 | G→A | L12L (TTG→TTA) | sbnA → | putative siderophore biosynthesis protein SbnA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 134,393 | 0 | G | A | 96.7% | 102.8 / NA | 30 | L12L (TTG→TTA) | sbnA | putative siderophore biosynthesis protein SbnA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/19); minor base C (1/0); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAC > USA300TCH1516_ALE/134257‑134516 | aaTGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAg < 1:382348/141‑1 (MQ=255)aaTGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAg < 1:309188/141‑1 (MQ=255) tGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGAt < 1:348586/141‑1 (MQ=255) cATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTc < 1:406284/141‑1 (MQ=255) tAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTAATTATTAGATTCTg > 1:308107/1‑141 (MQ=255) tAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTa < 1:127360/141‑1 (MQ=255) aTAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACt < 1:128825/141‑1 (MQ=255) gagaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatc < 1:434736/141‑1 (MQ=255) gagaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatc < 1:230252/141‑1 (MQ=255) agaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatca < 1:413277/141‑1 (MQ=255) agaATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTtcatca < 1:383465/141‑1 (MQ=255) aaTCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAAc < 1:96994/141‑1 (MQ=255) aTTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGaaa > 1:353461/1‑141 (MQ=255) aGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACAt < 1:348855/141‑1 (MQ=255) tAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATGGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGaa > 1:255281/1‑141 (MQ=255) attatAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGAGTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCaaa > 1:208269/1‑141 (MQ=255) acttttAAATTTGAGGAGGAAGCGCTGTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTagag < 1:173583/139‑1 (MQ=255) tAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTCTTAGATTCTGTAGGGCAAACACCTATGGTGTTACGTCATCAACTATTTCCGAAACATGAAGGGGTTGCAAAGTTAGAGTatat > 1:388278/1‑141 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg < 1:391959/141‑1 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg > 1:377012/1‑141 (MQ=255) aaaTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCACCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATg < 1:141228/141‑1 (MQ=255) aTTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCGAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGaa > 1:317659/1‑141 (MQ=255) gcgcTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGgcagc > 1:158187/1‑141 (MQ=255) gcgcTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACAACTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGgcagc > 1:442796/1‑141 (MQ=255) cTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATg < 1:306178/141‑1 (MQ=255) gattgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGa < 1:258163/141‑1 (MQ=255) ttgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAGCTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGGAAGATc > 1:85129/1‑141 (MQ=255) ttgaAAAAAGTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATc > 1:172943/1‑141 (MQ=255) gTCAAGCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCa < 1:483917/141‑1 (MQ=255) gCATGTCACGATTCATTATTAGATTCTGTAGGGCAAACACCTATGGTTCAACTAAAATACCCATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAc < 1:492015/141‑1 (MQ=255) | AATGCATAAAAAATATAATCCTAATTACTTGATAGTGAGAATCATTATCAATTAGGTAACACACAATATTATAGAATTTTAAATTTGAGGAGGAAGCGCTTTTGATTGAAAAAAGTCAAGCATGTCACGATTCATTGTTAGATTCTGTAGGGCAAACACCTATGGTTCAACTTCATCAACTATTTCCGAAACATGAAGTGTTTGCAAAGTTAGAGTATATGAATCCTGGAGGCAGCATGAAAGATCGACCTGCCAAGTAC > USA300TCH1516_ALE/134257‑134516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |