Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	607,884	G→A	R209* (CGA→TGA)	dck ←	Deoxyadenosine/deoxycytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	607,884	0	G	A	100.0%	60.0 / NA	20	R209* (CGA→TGA)	dck	Deoxyadenosine/deoxycytidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

TGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAG  >  USA300TCH1516_ALE/607751‑608020                                                                                                                                      |                                                                                                                                         tgtgAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTTAATAATTCAAGCAAtt                                                                                                                                   <  1:297148/141‑1 (MQ=255) tgtgAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAtt                                                                                                                                   <  1:199943/141‑1 (MQ=255)          aTGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATc                                                                                                                          >  1:103423/1‑141 (MQ=255)              aTTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACGTGTCGATATGTTTGAATAATTCAAACAATTTTGTTTATTATAg                                                                                                                      >  1:40824/1‑141 (MQ=255)                   aTAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACACTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCCATTTTGTTCATCATAGGAttt                                                                                                                 <  1:258365/141‑1 (MQ=255)                                  taatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGGTTTAAAGATTCGGGGTcc                                                                                                  >  1:265159/1‑141 (MQ=255)                                  taatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTGGTCGATATGTTGGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAGGATTCGGGGTcc                                                                                                  >  1:97818/1‑141 (MQ=255)                                            tatCTATGGAATATACATTAAATATAAACATATACGCTATGTCTTTAGTCTTTTATCATGTATCTACTTGTCGATATGTTTGAATAATTGAAGCAATTTTGATTATCATAGGATTTAAAGATTCGGCGTCCTTATAGatat                                                                                        >  1:264119/1‑141 (MQ=255)                                                    gAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCa                                                                                <  1:160379/141‑1 (MQ=255)                                                            aTTGAATATAAACATATACGCTATGTCTTTAGTCGTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATAtt                                                                        <  1:196774/141‑1 (MQ=255)                                                                                   tGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCAt                                                 <  1:387467/141‑1 (MQ=255)                                                                                   tGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCAt                                                 <  1:502588/141‑1 (MQ=255)                                                                                                 gaTCGTGTATCTACTTGTCGAGATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGACTTAAAGATTCGGGGTCCTTATGGATATCATATTCAGTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATc                                   <  1:128272/140‑1 (MQ=255)                                                                                                       gtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTTATTAATATTGCTCCGCTCGACTGGACATGCATTAAAGCTATTAATCCAATCg                             <  1:117487/141‑1 (MQ=255)                                                                                                              cTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGc                      >  1:158525/1‑141 (MQ=255)                                                                                                              cTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGc                      >  1:158436/1‑141 (MQ=255)                                                                                                                   cGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTa                 >  1:431024/1‑141 (MQ=255)                                                                                                                          tttGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTGATAGCGTGTAAATAGCt          >  1:447593/1‑141 (MQ=255)                                                                                                                              aataatTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCttttt      >  1:410551/1‑141 (MQ=255)                                                                                                                                  atTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAGTCCAATCGTCATAGCGTTTAAATAGCTTTTTcaat  >  1:344982/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                         TGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAG  >  USA300TCH1516_ALE/607751‑608020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑