Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	629,681	C→A	L84I (CTA→ATA)	USA300HOU_RS02990 →	3‑hexulose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	629,681	0	C	A	100.0%	56.0 / NA	18	L84I (CTA→ATA)	USA300HOU_RS02990	3‑hexulose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/10);  total (8/10)

CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  USA300TCH1516_ALE/629545‑629818                                                                                                                                         |                                                                                                                                          cGAAGGTTCACCAGCAGTAAAACAAACGGAAGACAACACGAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAgg                                                                                                                                       <  1:91300/141‑1 (MQ=255)                                     caTTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTga                                                                                                   <  1:43460/141‑1 (MQ=255)                                            aaTGTAAAAGTATTAGCAGAAATGAAAATTATAGATGCAGCTGTTTATGAAGTTAGCCAAGAAATTAAATTTGGCGCGGATGTAATTATAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGTTGAAGcg                                                                                            >  1:50737/1‑140 (MQ=255)                                                  aaaGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      >  1:64037/1‑141 (MQ=255)                                                  aaaGTATCAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaa                                                                                      <  1:64399/141‑1 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:107799/1‑141 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:103901/1‑141 (MQ=255)                                                    aGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGAAGTTAAAGTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATaaaaa                                                                                    >  1:416479/1‑141 (MQ=255)                                                        ttAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGGGTTGCAGAAGAGGCATCAATTCAAGCAGCTATTGAAGAAGCTCATAAAaataat                                                                                >  1:12982/1‑141 (MQ=255)                                                          aGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGAAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAAtaataa                                                                              <  1:130013/141‑1 (MQ=255)                                                                    aaaaTTATAGATGCAGCTGAATATGAAGTTAGCCAAGCAATTAAGTTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCACCAATTAAAGCAGCTACTGAAGAAACTCATAAAAATAATAAACAATTACTa                                                                    >  1:303310/1‑141 (MQ=255)                                                                          ataGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGat                                                              <  1:17613/141‑1 (MQ=255)                                                                             gATGCAGATGATTATGAAGTTAGACAAGCAATTAAATTTGGCGAGGATGTAATTACAATAATAGGTGTTAGAGAAGATGCATCATATGCATCTGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATg                                                           <  1:366233/141‑1 (MQ=255)                                                                                  aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:497961/141‑1 (MQ=255)                                                                                  aGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGc                                                      <  1:15058/141‑1 (MQ=255)                                                                                   gCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCt                                                     <  1:43816/141‑1 (MQ=255)                                                                                    cTGATTATGAAGTTAGACAAGCAATTAAATTTGGCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTg                                                    <  1:312246/141‑1 (MQ=255)                                                                                                                    ggCGCGGATGTAATTACAATAATAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGACAAACGTGCAAAAGAACTa                    >  1:441737/1‑141 (MQ=255)                                                                                                                                      ataataGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAt  <  1:326798/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          CGAAGGTTTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATGAAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTAATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCATAAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCAAAAGAACTAGATGAAATGGGTGCTGAT  >  USA300TCH1516_ALE/629545‑629818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑