Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 707,910 A→G 100% intergenic (‑1/‑121) znuC_1 ← / → ideR High‑affinity zinc uptake system ATP‑binding protein ZnuC/Iron‑dependent repressor IdeR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE707,9100AG99.1% 114.2 / ‑6.5 35intergenic (‑1/‑121)znuC_1/ideRHigh‑affinity zinc uptake system ATP‑binding protein ZnuC/Iron‑dependent repressor IdeR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (16/18);  minor base C (1/0);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAA  >  USA300TCH1516_ALE/707782‑708046
                                                                                                                                 |                                                                                                                                         
gAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTACCAAGCGTTTATAcctc                                                                                                                               >  1:262176/1‑141 (MQ=255)
        aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                       <  1:630834/141‑1 (MQ=255)
        aCCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTGCTAACAAGCGTTTATACCTCCTAATTaa                                                                                                                       >  1:434665/1‑141 (MQ=255)
             cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGtt                                                                                                                  <  1:682785/141‑1 (MQ=255)
              aCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGttt                                                                                                                 <  1:198844/141‑1 (MQ=255)
                     gggCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTaa                                                                                                          >  1:30798/1‑141 (MQ=255)
                       gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTGTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                        <  1:596087/141‑1 (MQ=255)
                         ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATATTCCGATCTGTGCAGGCGTTTCTACCTCCCAATTAAAAGTTTCGGCTAACCta                                                                                                      >  1:359524/1‑141 (MQ=255)
                          cctGATATCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:710545/139‑1 (MQ=255)
                          cATGATACCAATTATTTCGCCGCGTACTGGTATTGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:188175/141‑1 (MQ=255)
                          cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaa                                                                                                     <  1:240783/141‑1 (MQ=255)
                          cATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTGTATACCTCCTAATTAAAAGGTTAGGCGAACCtaa                                                                                                     >  1:314641/1‑141 (MQ=255)
                                aCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCCaattaatt                                                                                               >  1:345053/1‑141 (MQ=255)
                                 ccAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTACTGACTGTCCGTTTTTTTCTACTACTTAGAACATTAGGCTAACCTAATTAATTg                                                                                              >  1:552555/1‑141 (MQ=255)
                                        tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataata                                                                                       <  1:538842/141‑1 (MQ=255)
                                          tCGCCGCGTACTGGTATCGAGAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAGCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCCAACCTAATTAATTGTATAATaaa                                                                                     >  1:482150/1‑141 (MQ=255)
                                                                      tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                         >  1:642847/1‑141 (MQ=255)
                                                                      agTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTCAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                         <  1:638033/140‑1 (MQ=255)
                                                                               gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                                <  1:498619/141‑1 (MQ=255)
                                                                                          ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                    <  1:332087/141‑1 (MQ=255)
                                                                                          ttaCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACaa                                    >  1:501828/1‑141 (MQ=255)
                                                                                            aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                  <  1:216799/141‑1 (MQ=255)
                                                                                            aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAGCGTTTACACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                  <  1:216800/141‑1 (MQ=255)
                                                                                                 aaaaCAGATTTAAATCTTTTGTTTCTAACAAGCGTTTAGACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATCTAATATAGACAatgtatgt                             <  1:497155/141‑1 (MQ=255)
                                                                                                       gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                       <  1:280726/141‑1 (MQ=255)
                                                                                                       gATTTAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                       >  1:647177/1‑141 (MQ=255)
                                                                                                         tttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGCATATTTATCTTGTCAAGTAAATTCGTGATATAATATACACAATGTATGGGAGGTGaa                     >  1:164114/1‑141 (MQ=255)
                                                                                                          ttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTGATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCGTGTCAAGTAAATTCGTGATATAATATGAGAAATGTGAGTGAGGCGaaa                    >  1:240223/1‑141 (MQ=255)
                                                                                                          ttAAATCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGaaa                    >  1:420819/1‑141 (MQ=255)
                                                                                                               tCTTTTGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATg               <  1:656264/141‑1 (MQ=255)
                                                                                                                  tttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTa            <  1:696388/141‑1 (MQ=255)
                                                                                                                   ttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAGTTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTCTGTTaa           >  1:667350/1‑141 (MQ=255)
                                                                                                                   ttGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa           >  1:301750/1‑141 (MQ=255)
                                                                                                                    tGTTTCTAACAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAAc          <  1:152884/141‑1 (MQ=255)
                                                                                                                            aCAAGCGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGaaa  >  1:231228/1‑141 (MQ=255)
                                                                                                                                 |                                                                                                                                         
GAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAAA  >  USA300TCH1516_ALE/707782‑708046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: