Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 607,884 G→A 100% R209* (CGA→TGA)  dck ← Deoxyadenosine/deoxycytidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE607,8840GA100.0% 132.4 / NA 39R209* (CGA→TGA) dckDeoxyadenosine/deoxycytidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/22);  total (17/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAGT  >  USA300TCH1516_ALE/607750‑608021
                                                                                                                                      |                                                                                                                                         
atGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAAt                                                                                                                                     <  1:233761/141‑1 (MQ=255)
          aTGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATc                                                                                                                           <  1:615736/141‑1 (MQ=255)
           tGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAGTATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTGATCGTGTATCTACTTTTCTATATGTTTGAATAATTCAAGGAATTTTGTTTATCa                                                                                                                          >  1:180992/1‑141 (MQ=255)
            gTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATATACTTGACAATATTTTTGAATAATTCAAGCAATTTTGTTTATCAt                                                                                                                         >  1:140288/1‑141 (MQ=255)
                tCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGATTAATTCAAGCAATTTTGTTTATCATAGGa                                                                                                                     >  1:743191/1‑141 (MQ=255)
                tCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGa                                                                                                                     >  1:413517/1‑141 (MQ=255)
                    aaGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTa                                                                                                                 <  1:234765/140‑1 (MQ=255)
                            aTAAAATAATCGATAATATCCATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCGCCTTGTCAATATTTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAATGATTCg                                                                                                         >  1:478212/1‑141 (MQ=255)
                             aaaaaTAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCgg                                                                                                        <  1:482346/140‑1 (MQ=255)
                              aaaaTAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCggg                                                                                                       >  1:131326/1‑141 (MQ=255)
                                aataatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGt                                                                                                     >  1:17163/1‑141 (MQ=255)
                                 ataatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTc                                                                                                    >  1:423739/1‑141 (MQ=255)
                                  taatCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTcc                                                                                                   >  1:610299/1‑141 (MQ=255)
                                        ataataTCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAGGCAATTTTGTTTATCATAGTATTTGAAGATTCGGGGTCCTTATgg                                                                                             >  1:31949/1‑141 (MQ=255)
                                            tatCAATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATCATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGatat                                                                                         >  1:520687/1‑141 (MQ=255)
                                             agcTATGGAACATACATTGAATATAAACATATACGCTATGTCTTTAGTCCTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATc                                                                                        <  1:20647/139‑1 (MQ=255)
                                               cTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCat                                                                                      <  1:634555/141‑1 (MQ=255)
                                                  tGGACTATACATGGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACGTGTCGATATGTTTGAACAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATAtt                                                                                   <  1:236378/141‑1 (MQ=255)
                                                      atatACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCAtt                                                                               <  1:720644/141‑1 (MQ=255)
                                                             ttGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTg                                                                        >  1:74070/1‑141 (MQ=255)
                                                              tGAATATAAACATATACGCTATGTTTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGa                                                                       <  1:472291/141‑1 (MQ=255)
                                                                   ataAACATATACGCTATGTCTTTAGTCTTTTACCGTGTATCTACTTGTCGATATGTTTGAATGATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGt                                                                  >  1:548143/1‑141 (MQ=255)
                                                                     aaaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTAc                                                                <  1:297037/141‑1 (MQ=255)
                                                                      aaCATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACa                                                               <  1:741137/141‑1 (MQ=255)
                                                                        cATATACGCTATGTCTTTAGTCTTTGATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAAc                                                             <  1:578833/141‑1 (MQ=255)
                                                                           ataCGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTgg                                                          <  1:23892/141‑1 (MQ=255)
                                                                              cGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACa                                                       <  1:73550/141‑1 (MQ=255)
                                                                              cGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACa                                                       <  1:430631/141‑1 (MQ=255)
                                                                              cGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATACTGATACGTACAACTGGACa                                                       <  1:570389/141‑1 (MQ=255)
                                                                              cGCTATGTCTTTAGTCTTTTATCGTGTAACTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCATTATGGATATCATATTCATTAATATTGATACGTACAACTGGACa                                                       <  1:432093/141‑1 (MQ=255)
                                                                                cTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATg                                                     <  1:507399/141‑1 (MQ=255)
                                                                                                     gtgtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAAt                                >  1:645622/1‑141 (MQ=255)
                                                                                                     gtgtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGCACACATGGACATGCATTCAAGCTATTAATCCAAt                                >  1:711401/1‑141 (MQ=255)
                                                                                                       gtATCTACTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCATTCg                              >  1:746210/1‑141 (MQ=255)
                                                                                                             aCTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATTATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAg                        <  1:128261/141‑1 (MQ=255)
                                                                                                             aCTTGTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTCCAGCTGGACATGCATTAAAGCTATTAATCCAATCGTCATAg                        >  1:139900/1‑141 (MQ=255)
                                                                                                                 gTCGATATGTTTGAATAATTCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGtt                    <  1:198584/141‑1 (MQ=255)
                                                                                                                        tGTTTGAACAATTCAAGCAATTTTGTTTATCATAGGATTTAAATATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAg             <  1:486345/141‑1 (MQ=255)
                                                                                                                                   ttCAAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAGt  <  1:606208/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
ATGTGAATTAATGTATTCGATAGAATTGATAAAATAATCGATAATATCTATGGAATATACATTGAATATAAACATATACGCTATGTCTTTAGTCTTTTATCGTGTATCTACTTGTCGATATGTTTGAATAATTCGAGCAATTTTGTTTATCATAGGATTTAAAGATTCGGGGTCCTTATGGATATCATATTCATTAATATTGATACGTACAACTGGACATGCATTAAAGCTATTAATCCAATCGTCATAGCGTTTAAATAGCTTTTTCCAGT  >  USA300TCH1516_ALE/607750‑608021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑