Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,371,220	C→T	G95D (GGT→GAT)	rpsJ ←	30S ribosomal protein S10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,371,220	0	C	T	100.0%	76.1 / NA	21	G95D (GGT→GAT)	rpsJ	30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/7);  total (14/7)

TACTACTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACACCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGGATGA  >  USA300TCH1516_ALE/2371084‑2371352                                                                                                                                         |                                                                                                                                     tactacTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGa                                                                                                                                  >  1:13441/1‑141 (MQ=255)   ctactGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATg                                                                                                                                <  1:208570/141‑1 (MQ=255)       tGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTaa                                                                                                                            >  1:289268/1‑141 (MQ=255)       tGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTaa                                                                                                                            >  1:289244/1‑141 (MQ=255)                                    cGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGttt                                                                                               >  1:171739/1‑141 (MQ=255)                                       aTACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTg                                                                                            >  1:6862/1‑141 (MQ=255)                                                 cATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGtt                                                                                  <  1:131952/141‑1 (MQ=255)                                                 cATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGtt                                                                                  <  1:237128/141‑1 (MQ=255)                                                    cccAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTAc                                                                               >  1:231794/1‑141 (MQ=255)                                                                            tttGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTgtgtgt                                                       <  1:206779/141‑1 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGCATTGttaa                                    >  1:99739/1‑139 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCa                                    <  1:296975/141‑1 (MQ=255)                                                                                               cctcctAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCa                                    >  1:113188/1‑141 (MQ=255)                                                                                                    tAAAATTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTCAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGa                               >  1:375557/1‑141 (MQ=255)                                                                                                        aTTGTCTATTATAATTTGATTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCt                           >  1:163076/1‑141 (MQ=255)                                                                                                                          aTTTCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggca         <  1:88583/141‑1 (MQ=255)                                                                                                                           tttCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGCTTAAGCGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggcac        >  1:106523/1‑141 (MQ=255)                                                                                                                           tttCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATgcacggcac        >  1:385821/1‑141 (MQ=255)                                                                                                                             tCGATGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGcacggcacgg      <  1:377479/141‑1 (MQ=255)                                                                                                                                aTGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGgatg   >  1:214400/1‑141 (MQ=255)                                                                                                                                 tGTCTACATCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGgatgt  >  1:2407/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TACTACTGTTACAGGGATTAATTCACCGTTTTCTCCGAATACTTGTGTCATCCCAATTTTTCTTCCTAAGATTCCTTTGGTCATCGAAAGTCCACCTCCTAAAATTGTCTATTATAATTTGATTTCGATGTCTACACCAGATGGTAAGTTTAAGCCCATTAAAGCGTCAACTGTTTTTGGTGTTGGGTTTACAATATCGATTAAACGTTTGTGTGTACGTTGTTCGAATTGTTCACGTGAATCTTTATACTTATGCACGGCACGGATGA  >  USA300TCH1516_ALE/2371084‑2371352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑