Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,816,469	G→C	Y620* (TAC→TAG)	sraP ←	Serine‑rich adhesin for platelets

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,816,469	0	G	C	100.0%	82.6 / NA	22	Y620* (TAC→TAG)	sraP	Serine‑rich adhesin for platelets
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/12);  total (10/12)

GGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCT  >  USA300TCH1516_ALE/2816335‑2816604                                                                                                                                       |                                                                                                                                        ggTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACtt                                                                                                                                   <  1:269573/141‑1 (MQ=255)                      tttATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTa                                                                                                              <  1:314540/141‑1 (MQ=255)                                     gtcgtcGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTcaca                                                                                               >  1:295136/1‑141 (MQ=255)                                       cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  1:380046/141‑1 (MQ=255)                                       cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             <  1:277535/141‑1 (MQ=255)                                       cgtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGt                                                                                             >  1:281749/1‑141 (MQ=255)                                        gtcgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTc                                                                                            <  1:165146/141‑1 (MQ=255)                                          cgATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTccc                                                                                          <  1:108925/141‑1 (MQ=255)                                           gATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCa                                                                                         >  1:239273/1‑141 (MQ=255)                                                         ttGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGt                                                                           >  1:286728/1‑141 (MQ=255)                                                                     cacaACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatac                                                               <  1:192184/141‑1 (MQ=255)                                                                     cacaACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatac                                                               <  1:221277/141‑1 (MQ=255)                                                                         aCTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatacaata                                                           >  1:390173/1‑141 (MQ=255)                                                                         aCTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTcaatacaata                                                           >  1:2979/1‑141 (MQ=255)                                                                                cTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCa                                                    <  1:117481/141‑1 (MQ=255)                                                                                 tGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCAt                                                   >  1:140931/1‑141 (MQ=255)                                                                                 tGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCAt                                                   >  1:140930/1‑141 (MQ=255)                                                                                    tgattgaCCACTTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCCTCCCTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGt                                                <  1:293929/141‑1 (MQ=255)                                                                                                       ggTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                             <  1:187247/141‑1 (MQ=255)                                                                                                       ggTGTCCCAATGATTGAATTCGTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGtt                             >  1:12245/1‑141 (MQ=255)                                                                                                                            gTTGCACTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTacagttacagtt        <  1:312171/141‑1 (MQ=255)                                                                                                                                  cTATCCTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCt  >  1:392170/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                        GGTGCTGTCGTATCCACAACATTTATTGTAAAAGTTGTCGTCGATTTGTTATTTGCTTGGTCAGTAGACACAACTGTCACTGTTGATTGACCAATTTTTGTTGGTGTCCCAATGATTGAATTCGTTGCACTATCGTAACTTAATCCGCTTGGTAATCCTGTAACTGTATTTGTCACAGTCCCAGTACCATTATCCGTTGTAGTCAATACAATAGGATTCATTGTTTTACCCACTTCTATGGTTTGATTGCCTACAGTTACAGTTGGTGCT  >  USA300TCH1516_ALE/2816335‑2816604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑