Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,430,691:1	+A	8.2%	intergenic (‑115/‑270)	suhB_2 ← / → birA_2	Inositol‑1‑monophosphatase/Bifunctional ligase/repressor BirA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,430,691	1	.	A	8.2%	241.1 / 15.1	61	intergenic (‑115/‑270)	suhB_2/birA_2	Inositol‑1‑monophosphatase/Bifunctional ligase/repressor BirA
Reads supporting (aligned to +/- strand):  ref base . (26/30);  new base A (2/3);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

TATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGCA  >  USA300TCH1516_ALE/2430550‑2430829                                                                                                                                               |                                                                                                                                           tATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAG‑CACGTg                                                                                                                                             <  1:544299/141‑1 (MQ=255)   tttGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑c                                                                                                                                           <  1:388079/141‑1 (MQ=255)     tGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTa                                                                                                                                         >  1:630372/1‑141 (MQ=255)        tGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGcc                                                                                                                                      <  1:694447/141‑1 (MQ=255)         gTAATGTTTTATCTGTCATTTCTTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCg                                                                                                                                     <  1:764980/141‑1 (MQ=255)         gTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCg                                                                                                                                     <  1:93007/141‑1 (MQ=255)          tAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAG‑CACGTGACTAGCCGa                                                                                                                                    <  1:290454/141‑1 (MQ=255)          tAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAG‑CACGTGACTAGCCGa                                                                                                                                    <  1:528775/141‑1 (MQ=255)           aaTGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAG‑CACGTGACTAGCCGat                                                                                                                                   <  2:147208/141‑2 (MQ=255)              gTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAATGATCGTCTAGTCACGTG‑CTAGCCGACaaa                                                                                                                                <  2:75900/141‑1 (MQ=255)               ttttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAAt                                                                                                                               <  1:307339/141‑1 (MQ=255)               ttttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAAt                                                                                                                               >  1:151840/1‑141 (MQ=255)                tttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATa                                                                                                                              <  2:44455/141‑1 (MQ=255)                tttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATa                                                                                                                              >  1:343426/1‑141 (MQ=255)                tttATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACACTGTTTTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATa                                                                                                                              <  1:445988/141‑1 (MQ=255)                    tCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAgaaa                                                                                                                          <  1:516021/141‑1 (MQ=255)                       gTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAACAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAgaaagga                                                                                                                       >  2:316509/1‑141 (MQ=255)                       gTCATTTCGTCCACCACTTCTCATATAATTATCATTTTAATATGACTCTATAGTAAAAGAATCAACAATACAACTGATGACTTCTTCATTTTATGCATAAAAAAAACGGCTAGTCAAGTG‑CTAGCCGACAAATAgaaagga                                                                                                                       <  1:705089/141‑1 (MQ=255)                        tCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAgaaaggaa                                                                                                                      >  1:774787/1‑141 (MQ=255)                        tCATTTCGTCCACCACTTCTCATATCATTATCATTTTATGATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAgaaaggaa                                                                                                                      >  1:21637/1‑141 (MQ=255)                           tttCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAaagt                                                                                                                   <  1:625666/141‑1 (MQ=255)                             tCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAaagtaa                                                                                                                 >  1:220615/1‑141 (MQ=255)                              cGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAaagtaag                                                                                                                >  1:297423/1‑141 (MQ=255)                                  caccacTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGtaat                                                                                                            <  1:34296/141‑1 (MQ=255)                                         tctcATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATAtt                                                                                                     <  2:261233/141‑1 (MQ=255)                                            cATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGACAGTAAGTAATAAATATTGaa                                                                                                  >  1:32856/1‑141 (MQ=255)                                            cATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGaa                                                                                                  <  1:603887/141‑1 (MQ=255)                                             atatCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAg                                                                                                 <  1:492549/141‑1 (MQ=255)                                                  attatcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGAtgtt                                                                                            <  2:313051/141‑1 (MQ=255)                                                   ttatcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGAtgttg                                                                                           >  2:238882/1‑141 (MQ=255)                                                   ttatcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGAtgttg                                                                                           <  2:281474/141‑1 (MQ=255)                                                      tcattTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGa                                                                                        <  1:311254/141‑1 (MQ=255)                                                         ttttATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGt                                                                                     >  2:204618/1‑141 (MQ=255)                                                           ttattattaCCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTaa                                                                                   >  1:632683/1‑141 (MQ=255)                                                                     ccTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGAt                                                                         <  1:554749/141‑1 (MQ=255)                                                                           ttAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGGAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAg                                                                   <  1:378782/141‑1 (MQ=255)                                                                             aaaaGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCt                                                                 <  2:205885/141‑1 (MQ=255)                                                                               aaGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTAt                                                               <  1:731238/141‑1 (MQ=255)                                                                               aaGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTAt                                                               >  1:539070/1‑141 (MQ=255)                                                                                    tCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGtt                                                          <  1:70919/141‑1 (MQ=255)                                                                                       acaaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtata                                                       <  1:75721/141‑1 (MQ=255)                                                                                        caaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGGGAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtatat                                                      >  1:274174/1‑141 (MQ=255)                                                                                        caaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtatat                                                      >  1:607673/1‑141 (MQ=255)                                                                                        caaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtatat                                                      >  1:374077/1‑141 (MQ=255)                                                                                        caaTACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTtatat                                                      >  2:238123/1‑141 (MQ=255)                                                                                             cAACTGAAGACTTCTTCATTTGATGCGCAAAAAAAGCGGCGAGTCAGTTG‑CGATCCAGCAGAGAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGctc                                                 <  1:271185/141‑1 (MQ=255)                                                                                                            tcATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTaa                                  >  1:134559/1‑141 (MQ=255)                                                                                                              aTTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATc                                <  1:259992/141‑1 (MQ=255)                                                                                                                 ttATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAATCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCt                             <  2:333574/141‑1 (MQ=255)                                                                                                                 ttATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCt                             <  2:25854/141‑1 (MQ=255)                                                                                                                   aTGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTcc                           >  2:254736/1‑141 (MQ=255)                                                                                                                    tGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTccc                          >  1:717273/1‑141 (MQ=255)                                                                                                                       aTAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCt                       <  1:183442/141‑1 (MQ=255)                                                                                                                                tCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGt              <  1:231115/141‑1 (MQ=255)                                                                                                                                 cGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTa             >  2:207123/1‑141 (MQ=255)                                                                                                                                 cGGCTAG‑CACGTGACTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTa             >  2:315681/1‑141 (MQ=255)                                                                                                                                   gCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAat           <  1:524995/141‑1 (MQ=255)                                                                                                                                    cTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAata          >  2:91897/1‑141 (MQ=255)                                                                                                                                      tagcACGTGACTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAatat         >  2:89439/4‑141 (MQ=255)                                                                                                                                            gTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGca  >  2:145128/1‑141 (MQ=255)                                                                                                                                            gTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGca  >  2:145124/1‑141 (MQ=255)                                                                                                                                            gTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGca  >  2:117527/1‑141 (MQ=255)                                                                                                                                            gTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGca  >  1:782656/1‑141 (MQ=255)                                                                                                                                               |                                                                                                                                           TATTTGCTGTAATGTTTTATCTGTCATTTCGTCCACCACTTCTCATATCATTATCATTTTATTATTACCCTATATTAAAAGAATCAACAATACAACTGAAGACTTCTTCATTTTATGCATAAAAAAATCGGCTAGTCACGTG‑CTAGCCGACAAATAGAAAGGAAAGTAAGTAATAAATATTGAAGATGTTGTGATGTAACTTGAACGATTAAAAGCTATCTGTTATATAGCTCTACCCCTTTGTTTAATCGCTCCCCCTGTTACAAGTAATATCATAGCA  >  USA300TCH1516_ALE/2430550‑2430829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑