Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	396,540	G→A	80.4%	A208T (GCA→ACA)	efeM →	putative iron uptake system component EfeM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	396,540	0	G	A	80.4%	151.3 / 27.6	66	A208T (GCA→ACA)	efeM	putative iron uptake system component EfeM
Reads supporting (aligned to +/- strand):  ref base G (8/5);  new base A (30/23);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  USA300TCH1516_ALE/396404‑396675                                                                                                                                         |                                                                                                                                         aTTAGATATCATACCAAAATTAATGTTACTAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGcacaca                                                                                                                                      <  1:498790/141‑1 (MQ=255) aTTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAACTGAAGTTGCAACTTCTAAAATCTCAGGTGATGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGcacaca                                                                                                                                      <  2:114214/141‑1 (MQ=255)    aGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACaaaa                                                                                                                                   <  1:9298/141‑1 (MQ=255)         tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                              <  1:519837/141‑1 (MQ=255)           atgccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGCACGTTGAAGGCGCACAAAAAATTTAt                                                                                                                            >  1:626666/4‑141 (MQ=255)              ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAc                                                                                                                         <  2:181951/141‑1 (MQ=255)                    ttAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTAttt                                                                                                                   >  1:93421/1‑141 (MQ=255)                     tAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTa                                                                                                                  <  1:69894/141‑1 (MQ=255)                      aaTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaa                                                                                                                 <  2:193831/141‑1 (MQ=255)                       aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTaaa                                                                                                                >  1:345766/1‑141 (MQ=255)                         gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAAcc                                                                                                              <  2:93449/141‑1 (MQ=255)                            aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAt                                                                                                           >  1:721078/1‑141 (MQ=255)                                 gTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTag                                                                                                      <  2:290774/141‑1 (MQ=255)                                  ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTaga                                                                                                     <  1:154616/141‑1 (MQ=255)                                    cTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                   >  2:113230/1‑141 (MQ=255)                                    cTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                   >  1:559773/1‑141 (MQ=255)                                     ttccgatcTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  <  1:75146/134‑1 (MQ=255)                                     tGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  2:271260/1‑141 (MQ=255)                                     tGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaa                                                                                                  >  1:162091/1‑141 (MQ=255)                                       ttGACCTATTAAATGAAGTTGCAACTGCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaa                                                                                                <  1:2073/141‑1 (MQ=255)                                         gACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                              >  1:584900/1‑141 (MQ=255)                                         gACCTATTAAATGAAGTTGCAACTTCTAAAAGCACATGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaaaaa                                                                                              <  2:2260/141‑1 (MQ=255)                                           ccTATTAAATGAAGTTCATACGGCTAAAATCACAGGTGAAGAAGAAATTTATTCATATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTTAACCTATTTTAGAGAAAAAAGa                                                                                            <  1:6866/141‑1 (MQ=255)                                             tATTAAATGAAGTTGCAACTTCTAAAATAACCGGTGAAGAAGAAATTTCTTCACATACCGATTTATATGACTTTCCAGCGACCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑a                                                                                         <  1:590750/141‑1 (MQ=255)                                              aTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑aa                                                                                        <  1:449498/141‑1 (MQ=255)                                                     gAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTa                                                                                 <  2:96186/141‑1 (MQ=255)                                                       aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAg                                                                               >  1:517430/1‑141 (MQ=255)                                                       aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAg                                                                               >  2:170531/1‑141 (MQ=255)                                                       aGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAg                                                                               >  2:276081/1‑141 (MQ=255)                                                        gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGt                                                                              >  1:648653/1‑141 (MQ=255)                                                        gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGt                                                                              >  2:118119/1‑141 (MQ=255)                                                        gTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGt                                                                              >  1:132724/1‑141 (MQ=255)                                                         ttGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGtg                                                                             <  1:608387/141‑1 (MQ=255)                                                             aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTGACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AGAAAATTAAGtgatga                                                                         >  1:652959/1‑141 (MQ=255)                                                             aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGtgatga                                                                         >  1:195281/1‑141 (MQ=255)                                                             aaCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGtgatga                                                                         <  2:276786/141‑1 (MQ=255)                                                              aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTgatgat                                                                        >  1:784644/1‑141 (MQ=255)                                                                  cTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATcc                                                                    >  1:564227/1‑141 (MQ=255)                                                                     aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaaa                                                                 >  1:165553/1‑141 (MQ=255)                                                                     aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAACGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCaaa                                                                 >  1:596151/1‑141 (MQ=255)                                                                      aaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAAt                                                                >  1:545574/1‑141 (MQ=255)                                                                        acacaGGTGAAAAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAATTAAGTGATGATATCCAAATg                                                               <  1:519033/140‑1 (MQ=255)                                                                          acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAAc                                                            >  1:70890/1‑141 (MQ=255)                                                                           caGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACt                                                           >  1:346994/1‑141 (MQ=255)                                                                                gaagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGAt                                                      >  1:391802/1‑141 (MQ=255)                                                                                gaagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGAt                                                      <  2:102515/141‑1 (MQ=255)                                                                                   gaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATaaa                                                   >  1:730198/1‑141 (MQ=255)                                                                                          tttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATc                                            <  2:9775/141‑1 (MQ=255)                                                                                           ttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCa                                           >  2:110772/1‑141 (MQ=255)                                                                                              ttCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatt                                        <  1:100301/141‑1 (MQ=255)                                                                                                    aaCAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑CAAAAATTAAGTGATGATATCCAACTGAACTTCGATAAAGTGAATCAATTATTGGa                                  <  1:706045/140‑1 (MQ=255)                                                                                                     aCAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGAt                                 >  2:178872/1‑141 (MQ=255)                                                                                                      cAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATa                                <  1:190153/141‑1 (MQ=255)                                                                                                            tataTGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAAtata                          <  1:532903/141‑1 (MQ=255)                                                                                                             atatGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaa                         <  1:532007/141‑1 (MQ=255)                                                                                                              tatGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaa                        >  1:703334/1‑141 (MQ=255)                                                                                                               atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAg                       >  1:20337/1‑141 (MQ=255)                                                                                                                     ttAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaaca                 <  2:45347/141‑1 (MQ=255)                                                                                                                      tAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGGGAATCAATTATTGGATAAATATAAAGATaacaa                <  1:649078/141‑1 (MQ=255)                                                                                                                         aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg             >  2:175627/1‑141 (MQ=255)                                                                                                                                gTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATATTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATAATTGGATAAATATAAAGATAACAACGGCGGTTAt      >  1:447520/1‑141 (MQ=255)                                                                                                                                 ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg     >  1:458446/1‑141 (MQ=255)                                                                                                                                 ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg     >  1:682868/1‑141 (MQ=255)                                                                                                                                  tGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGa    >  1:765786/1‑141 (MQ=255)                                                                                                                                   gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg   >  1:447720/1‑141 (MQ=255)                                                                                                                                   gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg   >  1:328232/1‑141 (MQ=255)                                                                                                                                    aaGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGt  >  1:44404/1‑141 (MQ=255)                                                                                                                                    aaGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGt  <  1:752755/141‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAT‑AAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAGT  >  USA300TCH1516_ALE/396404‑396675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑