BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	110,904	G→T	6.7%	D255Y (GAT→TAT)	USA300HOU_RS00515 →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	110,904	0	G	T	6.7%	360.1 / 12.1	106	D255Y (GAT→TAT)	USA300HOU_RS00515	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base G (40/58); new base T (4/3); total (44/62)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

Only 100 of 110 total aligned reads displayed.

AAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  USA300TCH1516_ALE/110764‑111044

aaGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGgag                                                                                                                                              >  1:18593/1‑141 (MQ=255)

 aGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGTTAAAATGATAAAAAAAGCGgaga                                                                                                                                             >  1:6935/1‑141 (MQ=255)

   cTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATg                                                                                                                                           <  2:17646/141‑1 (MQ=255)

         aGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAAt                                                                                                                                     <  1:699427/141‑1 (MQ=255)

           ttATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCa                                                                                                                                   <  2:206309/141‑1 (MQ=255)

           ttATTGTTAAAAGGTTCACGGAATTTAAAAGGCTCATAAGTTGGATATAAAGATATTGAACTTACCTTTGTAGAGAAAAAAGAAGAAAATATCTACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCa                                                                                                                                   <  1:176823/141‑1 (MQ=255)

             aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 <  2:28340/141‑1 (MQ=255)

             aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 <  1:523564/141‑1 (MQ=255)

             aTTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATg                                                                                                                                 <  1:534884/141‑1 (MQ=255)

                   aaaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGTTATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCaa                                                                                                                           <  1:624849/141‑1 (MQ=255)

                    aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt                                                                                                                          >  2:334217/1‑141 (MQ=255)

                    aaaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAAt                                                                                                                          >  2:334285/1‑141 (MQ=255)

                     aaGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATc                                                                                                                         <  1:439768/141‑1 (MQ=255)

                       ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg                                                                                                                       <  2:126502/141‑1 (MQ=255)

                       ggTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTACAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAg                                                                                                                       <  2:203649/141‑1 (MQ=255)

                         ttCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGaa                                                                                                                     <  1:555906/141‑1 (MQ=255)

                         ttCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGaa                                                                                                                     >  2:242575/1‑141 (MQ=255)

                            aGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAatat                                                                                                                  >  1:402145/1‑141 (MQ=255)

                             gggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATg                                                                                                                 >  2:312602/1‑141 (MQ=255)

                             gggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATg                                                                                                                 >  1:309453/1‑141 (MQ=255)

                              ggAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGa                                                                                                                >  2:203146/1‑141 (MQ=255)

                                  tttAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatc                                                                                                            <  1:350383/141‑1 (MQ=255)

                                   ttAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcg                                                                                                           <  1:745981/141‑2 (MQ=255)

                                     aaaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaat                                                                                                         >  1:762612/1‑141 (MQ=255)

                                      aaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc                                                                                                        >  1:677299/1‑141 (MQ=255)

                                      aaaGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAaatcaatc                                                                                                        >  2:188283/1‑141 (MQ=255)

                                        aGGCTCATCAGTTGGATATAAAGATATTGAAGTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATccc                                                                                                      <  2:123067/141‑1 (MQ=255)

                                          gCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCgg                                                                                                    <  1:328412/141‑1 (MQ=255)

                                             catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa                                                                                                 <  2:212909/141‑1 (MQ=255)

                                             catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa                                                                                                 <  1:726158/141‑1 (MQ=255)

                                             catcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaa                                                                                                 <  1:680727/141‑1 (MQ=255)

                                               tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               >  2:41334/1‑141 (MQ=255)

                                               tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               >  1:240845/1‑141 (MQ=255)

                                               tcaGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGaaaaa                                                                                               <  2:298964/141‑1 (MQ=255)

                                                caGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAag                                                                                              <  1:161701/141‑1 (MQ=255)

                                                caGTTGGATATAAAGATATTGAATTTACGGTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAag                                                                                              >  1:755627/1‑141 (MQ=255)

                                                   ttGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAagaga                                                                                           <  2:133423/141‑1 (MQ=255)

                                                   ttGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAagaga                                                                                           <  1:368173/141‑1 (MQ=255)

                                                    tGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAagagag                                                                                          <  1:506894/141‑1 (MQ=255)

                                                       atatAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtga                                                                                       >  2:242325/1‑141 (MQ=255)

                                                       atatAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtga                                                                                       >  1:92683/1‑141 (MQ=255)

                                                        tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      >  2:232058/1‑141 (MQ=255)

                                                        tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      <  2:314866/141‑1 (MQ=255)

                                                        tataAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGtgat                                                                                      >  1:333596/1‑141 (MQ=255)

                                                           aaaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaa                                                                                   >  2:260407/1‑141 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAGAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGATATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  <  2:306807/141‑1 (MQ=255)

                                                            aaGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGaaa                                                                                  <  1:68754/141‑1 (MQ=255)

                                                              aatatTGAATTTACTTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATg                                                                                <  1:46413/140‑1 (MQ=255)

                                                               atatTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGa                                                                               >  2:109604/1‑141 (MQ=255)

                                                                    gAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATaaaa                                                                          >  2:181780/1‑141 (MQ=255)

                                                                      aTTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACg                                                                        >  1:204822/1‑141 (MQ=255)

                                                                         tACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACgtgt                                                                     >  1:471117/1‑141 (MQ=255)

                                                                           cGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaa                                                                   >  2:66461/1‑141 (MQ=255)

                                                                            gTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaa                                                                  <  1:29539/141‑1 (MQ=255)

                                                                            gTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaa                                                                  <  1:483266/141‑1 (MQ=255)

                                                                            gTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAAAGTGtaaa                                                                  >  1:342701/1‑141 (MQ=255)

                                                                                gTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaataaa                                                              <  1:142736/141‑1 (MQ=255)

                                                                                gTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaataaa                                                              >  1:528126/1‑141 (MQ=255)

                                                                                gTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGtaaataaa                                                              <  1:486315/141‑1 (MQ=255)

                                                                                  agagAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAAtt                                                            <  1:294835/141‑1 (MQ=255)

                                                                                    agaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAg                                                          <  1:439641/141‑1 (MQ=255)

                                                                                    agaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAAATTATAAAAAAAGAAAAACAGTCCAACCATAGATCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAg                                                          <  2:198215/141‑1 (MQ=255)

                                                                                     gaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGt                                                         >  1:685334/1‑141 (MQ=255)

                                                                                     gaAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGt                                                         >  2:32806/1‑141 (MQ=255)

                                                                                           agaagaAAATATATACTTTAGTGATAGCTTAGTTTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTgg                                                   >  1:382733/1‑141 (MQ=255)

                                                                                             aagaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTa                                                 <  1:454226/141‑1 (MQ=255)

                                                                                              agaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAt                                                >  1:466605/1‑141 (MQ=255)

                                                                                              agaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAt                                                >  1:587309/1‑141 (MQ=255)

                                                                                              agaaAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAt                                                <  1:637277/141‑1 (MQ=255)

                                                                                                 aaaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAg                                             >  2:15143/1‑141 (MQ=255)

                                                                                                  aaTATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGt                                            <  1:413571/141‑1 (MQ=255)

                                                                                                   atatatACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATTATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtc                                           <  1:237389/141‑1 (MQ=255)

                                                                                                   atatatACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtc                                           <  2:37277/141‑1 (MQ=255)

                                                                                                     atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  1:258010/141‑1 (MQ=255)

                                                                                                     atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  1:56320/141‑1 (MQ=255)

                                                                                                     atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  2:257513/141‑1 (MQ=255)

                                                                                                     atataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtctt                                         <  2:250209/141‑1 (MQ=255)

                                                                                                      tataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtcttc                                        <  1:320286/141‑1 (MQ=255)

                                                                                                       ataCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGtcttct                                       <  1:157655/141‑1 (MQ=255)

                                                                                                        taCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTg                                      >  1:102867/1‑141 (MQ=255)

                                                                                                         aCTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGt                                     >  1:308638/1‑141 (MQ=255)

                                                                                                          cTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGtt                                    <  1:324546/141‑1 (MQ=255)

                                                                                                           tttAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGttt                                   <  1:346223/141‑1 (MQ=255)

                                                                                                              aGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTGGTCTTCTGTTTTTa                                >  1:67941/1‑141 (MQ=255)

                                                                                                                tGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGt                              >  1:203864/1‑141 (MQ=255)

                                                                                                                      cTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAg                        <  1:579298/141‑1 (MQ=255)

                                                                                                                       ttAGATTATAACAAAAGCGGAGATGTCTAATCATGGCTCAATCAGAATATGGAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGt                       <  1:29275/141‑1 (MQ=255)

                                                                                                                             tataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCAct                 >  2:146180/1‑141 (MQ=255)

                                                                                                                             tataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCAct                 <  1:224120/141‑1 (MQ=255)

                                                                                                                              ataAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActg                >  2:56249/1‑141 (MQ=255)

                                                                                                                                aaaaaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgct              >  1:116258/1‑141 (MQ=255)

                                                                                                                                 aaaaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgctg             >  1:577564/1‑141 (MQ=255)

                                                                                                                                 aaaaaaGCGGAGATGTATAATCATGGATCAATCAGAATCTGAATTCAATCCCGGCAAAAGAGCGTTAAGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCActgctg             <  1:273154/141‑1 (MQ=38)

                                                                                                                                  aaaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTgg            <  1:108838/141‑1 (MQ=255)

                                                                                                                                   aaaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGt           <  1:700080/141‑1 (MQ=255)

                                                                                                                                    aaaGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGtt          <  2:5417/141‑1 (MQ=255)

                                                                                                                                      aGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTtgt        <  1:572964/141‑1 (MQ=255)

                                                                                                                                        aggAGATGTATAATCATGGCTCAATCAGAATATAAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTgg      <  2:222857/140‑1 (MQ=255)

                                                                                                                                         ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTAATAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGc     >  2:233296/1‑141 (MQ=255)

                                                                                                                                           agaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCCGGTTGTGGCAt   >  1:491733/1‑141 (MQ=255)

AAGCTCCAAAGTTATTGTTAAAAGGTTCAGGGAATTTAAAAGGCTCATCAGTTGGATATAAAGATATTGAATTTACGTTTGTAGAGAAAAAAGAAGAAAATATATACTTTAGTGATAGCTTAGATTATAAAAAAAGCGGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATG  >  USA300TCH1516_ALE/110764‑111044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`