| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 698,981 | G→T | M46I (ATG→ATT) | mrpC → | Na(+)/H(+) antiporter subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 698,981 | 0 | G | T | 100.0% | 23.5 / NA | 8 | M46I (ATG→ATT) | mrpC | Na(+)/H(+) antiporter subunit C |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (3/5); total (3/5) | |||||||||||
TATAGGAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATGGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTAT > USA300TCH1516_ALE/698885‑699122 | tataGGAACATATATGATTTTATCAATCAATTTAATTAGTATAGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCaa > 1:8338/1‑148 (MQ=255) ggAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAAttgt < 1:106722/148‑1 (MQ=255) aaCATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAAttgttt < 1:36973/148‑1 (MQ=255) atGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATc > 1:268528/1‑148 (MQ=255) gTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCACTATTATGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAAc > 1:108813/1‑148 (MQ=255) cTGGTAATCTCATTATTAGGATTGTTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGtt < 1:140046/148‑1 (MQ=255) tatGAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGtt < 1:247756/148‑1 (MQ=255) gAGTATTGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTtat < 1:171888/148‑1 (MQ=255) | TATAGGAACATATATGATTTTATCAATCAATTTAATTCGTATTGTAATCGGAATTTCAATATATACTCATGCTGGTAATCTCATTATTATGAGTATGGGAACGTATGGTTCTAGTAGATCAGAACCACTAATAACTGGTGGAAACCAATTGTTTGTTGATCCCTTGTTACAAGCTATTGTACTAACTGCAATAGTTATAGGGTTTGGGATGACTGCGTTTTTACTTGTACTTGTTTAT > USA300TCH1516_ALE/698885‑699122 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |