| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,522,796 | T→A | K171I (AAA→ATA) | dnaD ← | DNA replication protein DnaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,522,796 | 0 | T | A | 100.0% | 17.7 / NA | 6 | K171I (AAA→ATA) | dnaD | DNA replication protein DnaD |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (2/4); total (2/4) | |||||||||||
ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGA > USA300TCH1516_ALE/1522654‑1522894 | aTCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTc > 1:136985/1‑148 (MQ=255) aGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTc < 1:273969/148‑1 (MQ=255) cGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCg < 1:73894/148‑1 (MQ=255) gTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGta > 1:263778/1‑148 (MQ=255) cAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGt < 1:200738/148‑1 (MQ=255) gTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTATATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGga < 1:267117/148‑1 (MQ=255) | ATCAAATTTAGGTACCGTTTTAACTGTGTGCGTCATTTTCGGTTTATTGAATTTTTCTCTTATTTTTCTAGAATCGTCAATTGTTTTGACATTGTTTTTCTTCCAATTAAGTAAAATACGATCTATATATTTAAAGCTAAGTTTATTCAAACTATTCGCCTCGTCTAATGCCGCTTGTATAATTGCAGTATCGTGTTTATCAACATCAATCCATTGATTTAACGTTTCTATTTCATATGGA > USA300TCH1516_ALE/1522654‑1522894 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |