Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	1,977,348	G→C	L368V (CTA→GTA)	yhaO ←	putative metallophosphoesterase YhaO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,977,348	0	G	C	100.0%	45.3 / NA	13	L368V (CTA→GTA)	yhaO	putative metallophosphoesterase YhaO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (6/7);  total (6/7)

TCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGT  >  USA300TCH1516_ALE/1977205‑1977472                                                                                                                                                |                                                                                                                             tCAATTTTACGTTGAACATATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                          <  1:234159/148‑1 (MQ=255) tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                          <  1:172407/148‑1 (MQ=255) tCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAAc                                                                                                                          <  1:204990/148‑1 (MQ=255)          cGTTGAACAAATTGACCGTAACCATACATTTCAAGTGATTTCATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGcc                                                                                                                 <  1:124240/148‑1 (MQ=255)               aaCAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTgg                                                                                                            <  1:218561/148‑1 (MQ=255)                                               aTTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCaa                                                                            >  1:141074/1‑148 (MQ=255)                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                   >  1:214194/1‑148 (MQ=255)                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                   >  1:214214/1‑148 (MQ=255)                                                        tCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGttt                                                                   >  1:217825/1‑148 (MQ=255)                                                                 tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                          >  1:10238/1‑148 (MQ=255)                                                                 tCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACta                                                          >  1:194010/1‑148 (MQ=255)                                                                                                            cTAATGCTGTATGGTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCAtt               <  1:102748/148‑1 (MQ=255)                                                                                                                         gTCGAATGTTCCATAATCGTCTACGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGt  <  1:109665/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                             TCAATTTTACGTTGAACAAATTGACCGTAACCATAAATTTCAAGTGATTTAATTATCATTTTGTTCACCTCTCATTTCAGCTTTTAATATTTCTTCAGCACGATTAACTAATGCTGTATGGTCGAATGTTCCATAATCGTCTAGGAACTTAGATGCCCTTGGATTTAAATATAAATCTGACATCGCTTTATCAAAAACAGTTTGATCGACTAATAATTCCGCTGAAAATTCATTAACTAAAGGTGACTCATCATTTTGTGCATATTGT  >  USA300TCH1516_ALE/1977205‑1977472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑