| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,642,589 | G→A | intergenic (‑19/+120) | USA300HOU_RS13595 ← / ← yccS | hypothetical protein/Inner membrane protein YccS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,642,589 | 0 | G | A | 100.0% | 41.5 / NA | 13 | intergenic (‑19/+120) | USA300HOU_RS13595/yccS | hypothetical protein/Inner membrane protein YccS |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/6); total (7/6) | |||||||||||
GAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTC > USA300TCH1516_ALE/2642447‑2642729 | gAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATtata < 1:34257/148‑1 (MQ=255) tCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGt > 1:112612/1‑148 (MQ=255) tCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGt > 1:242410/1‑148 (MQ=255) tAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTGATGTTATATACCACCATAATTATaaaa > 1:294582/1‑148 (MQ=255) tttttGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCTCTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTCAACACATTTTTTTaa > 1:42443/1‑147 (MQ=255) tAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCat < 1:211735/148‑1 (MQ=255) aCTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCAtaataa > 1:58965/1‑148 (MQ=255) aTTTTTCTGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTATACTCCCGTCATAATAACTTCAGACAAACGTTa < 1:95329/148‑1 (MQ=255) gggTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGAcc < 1:279849/148‑1 (MQ=255) gTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTg < 1:189812/148‑1 (MQ=255) aTTTCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGt < 1:76572/148‑1 (MQ=255) tttCCTTAACCTCCAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGtt > 1:158959/1‑148 (MQ=255) ccAATATATTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTACCGTCCTAATAAATTCAGACACTCGTTAGTATGACCTGATAAATATTTAATTTTACACGTTCTTGtctctctc > 1:275445/1‑148 (MQ=255) | GAACAACATCTTTTTCAAGATATCCTTCTTTTAACATTGCATTCGCAATTTCTATCGCTTTTTGCCGTTTAATTACTTCAAATTCTTTCCATTTTTCGGGGTAACCTTTCATTGTAAAGGGCATTTCCTTAACCTCCAATATGTTATAATTCATGTTATATACCACCATAATTATAAAATGAAACATATTAAACACATTTTTTTAGACTCCCGTCATAATAACTTCAGACAAACGTTAGTCTGACCTGATAAATATTTAATTTTAAACGTTCTTGTCTCTCTC > USA300TCH1516_ALE/2642447‑2642729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |