| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 740,315 | Δ1 bp | 16.0% | intergenic (+542/+46) | pitA_1 → / ← sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 740,315 | 0 | C | . | 16.0% | 905.4 / 163.3 | 287 | intergenic (+542/+46) | pitA_1/sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Reads supporting (aligned to +/- strand): ref base C (96/144); new base . (5/41); total (102/185) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.12e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 299 total aligned reads displayed.
ATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGT > USA300TCH1516_ALE/740225‑740405 | aTACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAtc < 2:3539406/91‑1 (MQ=255)aTACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAtc < 1:207642/91‑1 (MQ=255) aCAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAtctc < 1:2812568/91‑1 (MQ=255) aCAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAtctc > 1:1760465/1‑91 (MQ=255) cAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTca > 2:3414650/1‑91 (MQ=255) aaaaTATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTcac > 2:1008898/1‑91 (MQ=255) aatataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACatc < 2:734514/91‑2 (MQ=255) aataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatga < 2:2696752/90‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:866273/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:2549669/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:2949419/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 1:1852429/91‑1 (MQ=255) aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGAtgt < 2:1487012/91‑1 (MQ=255) aaatatGTATGATAGGGAAGGGAGAGATAATTTTTTGTACCAAAATGAAAATTAATTAAAAAAATAAAAAACTAGCAAT‑TCACATCAtgtg < 2:3196504/91‑1 (MQ=255) aatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGAtgtg < 1:3473398/91‑1 (MQ=255) aatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGa < 1:1878913/91‑1 (MQ=255) aatatATATGATAGGGAAGGGTGAGATAATTTTTTGAACCAAAATGAAAATTTATTACAAAAAAAAAAAACTAGCAATCTCACATGAtgtg < 2:2446952/91‑1 (MQ=255) aagatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGa < 2:2790716/88‑1 (MQ=255) atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa > 2:3899492/1‑91 (MQ=255) atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGag < 1:3644150/91‑2 (MQ=255) atatGTATGATAGGGAAGGGTGAGAGAAGTTTAAGAACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGag < 2:35514/91‑2 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a < 2:1271369/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 2:3575578/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 2:1558468/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagat < 1:2159062/91‑4 (MQ=39) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagat < 1:3350024/91‑4 (MQ=39) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑Att < 1:1928475/91‑1 (MQ=255) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagatt < 1:1606894/91‑5 (MQ=38) tgtaggATAGGGAAGGGTTAGATAATTTTCCGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagatt < 2:963204/91‑5 (MQ=37) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg < 2:856651/91‑1 (MQ=255) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg > 1:3841211/1‑91 (MQ=255) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg > 1:588194/1‑91 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:1663954/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:2465830/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:2132497/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCt < 2:3295159/91‑1 (MQ=38) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAAGTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCt < 2:2350454/91‑1 (MQ=37) atgATAGGGAAGGGTGAGATAATATTTTGTACCAAAATAAAAATAATAAAAAAAAAAAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:3624519/91‑1 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTa > 1:2552963/1‑91 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTa > 1:1069154/1‑91 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTa < 2:906650/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAg < 1:1469223/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAg < 1:3809577/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAg < 2:651079/91‑1 (MQ=37) aTAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGt < 1:56159/91‑1 (MQ=37) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 1:1169987/91‑3 (MQ=25) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGttt < 2:2385811/91‑3 (MQ=25) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTGTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 2:3463600/91‑4 (MQ=25) ggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatat > 2:285652/1‑91 (MQ=255) gAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatata < 1:3578149/91‑1 (MQ=255) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc > 1:1060873/1‑91 (MQ=255) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc < 2:1811101/91‑1 (MQ=255) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTATAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc < 2:1826160/91‑1 (MQ=255) gggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCa < 1:832983/91‑1 (MQ=255) gggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCa > 2:1194957/1‑91 (MQ=255) ggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAg < 2:700640/91‑1 (MQ=255) gagagaTAATGTTTTGTCATAAAATGAAAATTTTATTAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt < 2:1933291/89‑1 (MQ=255) gTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt < 2:3494451/91‑1 (MQ=255) gagaTAATTTTTTGTAGCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGTAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTAc < 2:3780049/91‑1 (MQ=255) gaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACaa > 1:202166/1‑91 (MQ=255) tAATTATTTGTACCAAAATGAAAAATTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatt < 2:549829/91‑1 (MQ=255) aTTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAattta < 1:118930/91‑1 (MQ=255) ttttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAAATAACAATCTCACATGATGTGA‑ATTGCTAGTATATATAAGTACAatttat > 2:3374561/1‑91 (MQ=255) ttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttattt < 2:1057549/91‑1 (MQ=255) ttatGTACCAAAATGAAAA‑TTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta < 2:3046873/88‑1 (MQ=255) tttGTTCCAAAATGAAAATTTTTATAACAAATAAAAAACTAGCAATCTCACATGCTGTGA‑ATTGCTAGTATATATCAGTACAAtttattta > 2:2133132/1‑91 (MQ=255) tGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaat < 1:3726219/91‑1 (MQ=255) tACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaatta < 2:759524/91‑1 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 2:461308/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 1:1710850/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 2:3148865/1‑91 (MQ=255) aaaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGa < 1:1624736/91‑1 (MQ=255) aaaTGAAAAATTTATTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGa < 2:1365473/91‑1 (MQ=255) aaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGAt < 1:3135558/91‑1 (MQ=255) tGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGa > 1:2871970/1‑91 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 1:448293/91‑1 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAACCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACCATTTATTTAATTAATGGATGaa > 2:3189759/1‑91 (MQ=255) aaaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATg < 1:934749/91‑1 (MQ=255) aaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGc < 2:1642004/91‑1 (MQ=255) ttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATa > 1:660557/1‑91 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 1:508201/91‑1 (MQ=255) aaaaaaTAAAAGACTAGCAATCTTACATGATGTGA‑ATTGCTAGTATATATCAGTACAATGTATTTAATTAATGGATGAATGCATAGCTAGa < 2:3017823/90‑1 (MQ=255) aaaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa > 1:2722792/1‑91 (MQ=255) aaaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa > 2:1209295/1‑91 (MQ=255) aaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaaa > 1:2211353/1‑91 (MQ=255) aTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACtt < 2:3915812/91‑1 (MQ=255) aTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACtt < 2:1767387/91‑1 (MQ=255) aaaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCt < 1:240639/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTg > 1:1415150/1‑91 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATCGATGAATGCATAGCTAGAAACTTCTg < 2:2249643/91‑1 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa > 2:27407/1‑91 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg < 1:755246/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg > 2:2031211/1‑91 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc > 1:3939159/1‑91 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc < 1:285652/91‑1 (MQ=255) aCTAGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc > 2:2176624/1‑91 (MQ=37) cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCt < 1:3393742/91‑1 (MQ=255) tAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTg < 2:1981956/91‑1 (MQ=255) aGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg > 2:573699/1‑91 (MQ=38) aTCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAAtt < 1:1550188/91‑1 (MQ=255) | ATACAAAATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGT > USA300TCH1516_ALE/740225‑740405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |