| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 954,768 | A→T | 7.9% | intergenic (+422/+81) | gluD → / ← glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 954,768 | 0 | A | T | 7.9% | 780.1 / 41.4 | 240 | intergenic (+422/+81) | gluD/glpQ_1 | NAD‑specific glutamate dehydrogenase/Glycerophosphodiester phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (88/132); new base T (8/11); total (97/143) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 249 total aligned reads displayed.
ACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATG > USA300TCH1516_ALE/954678‑954858 | aCAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATaa < 1:20035/91‑1 (MQ=255)aCAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATaa < 2:1925403/91‑1 (MQ=255) tAGTGTGCAAGTTGGGGTGGGACGACTAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTc > 2:2238639/1‑91 (MQ=255) tAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTATGATAATTTc > 2:1113/1‑91 (MQ=255) gtgtGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCaa < 2:3459714/91‑1 (MQ=255) gtgtGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCaa > 2:1691762/1‑91 (MQ=255) tgtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAt > 2:2731884/1‑91 (MQ=255) tgtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAt < 2:2651226/91‑1 (MQ=255) tgtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAt < 2:2650916/91‑1 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt < 2:3862029/91‑1 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt < 1:156522/91‑1 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt < 2:3505339/91‑1 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt < 1:3093457/91‑1 (MQ=255) gtgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAtt > 1:852116/1‑91 (MQ=255) tgCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAAttt > 1:2340697/1‑91 (MQ=255) cAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTgg > 1:3200782/1‑91 (MQ=255) aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGaa < 2:718058/91‑1 (MQ=255) aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGaa > 1:3824862/1‑91 (MQ=255) aGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTACCAAAAAGACAGCAGTAGGATAATTTCAATTTGGaa < 2:1140862/91‑1 (MQ=255) tGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGCCAGCAGTAGGATAATTTCAATTTGGACata > 2:3562838/1‑91 (MQ=255) tGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAata < 1:3887254/91‑1 (MQ=255) ggggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAatat < 1:803693/91‑1 (MQ=255) gggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATc > 2:1345614/1‑91 (MQ=255) gggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATc > 1:373744/1‑91 (MQ=255) gggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTAAAAATAAAAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATc < 2:3450174/91‑1 (MQ=21) ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACCGCAGTAGGATAATTTCAATTAGGAAatatgt > 1:2216962/1‑89 (MQ=255) ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCt < 1:2608751/91‑1 (MQ=255) ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCt > 2:558478/1‑91 (MQ=255) ggTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAAGATATTTCCAAATTGAAATTATcc < 1:929512/91‑2 (MQ=17) gTGGGACGACGAAATAAATTTTGCGAAGATTTCATTTCTGTCCCACTCAAGAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCtt < 2:3666978/91‑1 (MQ=21) gTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCtt < 2:2208700/91‑1 (MQ=255) gTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCtt > 1:344150/1‑91 (MQ=255) gTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGCCCCACTCCCAAAAAGACAGCAGTAGGCTAATTTCAATTTGGAAATATccc > 2:652858/1‑89 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc < 2:2895199/91‑1 (MQ=255) gggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAc < 1:1766161/91‑1 (MQ=255) ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAct < 1:2262156/91‑1 (MQ=255) ggACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAct > 1:2566455/1‑91 (MQ=255) gacgacGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActg < 2:632348/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgc < 2:3212824/91‑1 (MQ=255) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgc < 2:3354671/91‑1 (MQ=14) acgacgAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgc < 2:1400834/91‑1 (MQ=17) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTActgctg < 1:1924023/91‑1 (MQ=255) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATTATCCTActgctg < 1:1851459/91‑1 (MQ=14) gacgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAAACAGCAGTAAGATATTTCCAAATTGAAATCATCCTActgctg < 1:3029696/91‑1 (MQ=9) cgaAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtt > 1:3486307/1‑91 (MQ=255) cgaAATAAATGTTGCGAAAATATCATTTCTGTCCCACTCCAAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtt < 2:2852727/91‑1 (MQ=255) aaataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttt > 2:1639915/1‑91 (MQ=255) aaataaatTTTGCGAAAATATCATTTCTGTACAAATAAAAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttt < 2:3367894/91‑1 (MQ=37) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTAGCGCTGtttttt > 2:3017930/1‑91 (MQ=255) ataaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGtttttt > 1:13601/1‑91 (MQ=255) taaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTta > 2:412323/1‑91 (MQ=255) aaatTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTtat < 1:334020/91‑1 (MQ=255) ttttGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGt > 1:3725168/1‑91 (MQ=255) tCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATAttt > 2:1844426/1‑91 (MQ=255) tttCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTg < 1:430937/91‑1 (MQ=255) tCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTa < 2:1797118/91‑1 (MQ=255) tCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTa < 1:314284/91‑1 (MQ=255) cTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAt < 2:1717916/91‑1 (MQ=255) cTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAt < 1:1638215/91‑1 (MQ=255) cTGTCCCACTCCC‑AAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 2:3250474/91‑1 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt > 2:910085/1‑91 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 1:2032085/91‑1 (MQ=255) tGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTAtt < 2:3307025/91‑1 (MQ=255) gTCCCACTCCCTAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGGTTTTTATAGTAATAGCTATATTTTTGTAttt < 2:3024889/91‑1 (MQ=255) tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTa < 1:1319529/91‑1 (MQ=255) tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTa < 1:1071995/91‑1 (MQ=255) tCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTa < 2:523719/91‑1 (MQ=255) cccAGTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTAt < 2:3637860/91‑1 (MQ=255) cccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTAt < 2:764551/91‑1 (MQ=255) cccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTAt < 1:3539409/91‑1 (MQ=255) ccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATg > 2:3717954/1‑91 (MQ=255) ccACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATg < 1:2739316/91‑1 (MQ=255) cACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGt > 1:2696223/1‑91 (MQ=255) acacAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGtttt < 2:3583014/88‑1 (MQ=255) acaaaaaaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGtttt < 2:2793902/87‑1 (MQ=255) cccAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTc < 1:144379/91‑1 (MQ=255) taaaaaaCAGCAGTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAg > 2:3386727/2‑91 (MQ=14) taaaaaaCAGCAGTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTCTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAg > 1:2747602/2‑91 (MQ=0) aaaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGt < 1:2402992/91‑1 (MQ=255) aaaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGt < 1:70232/91‑1 (MQ=255) aaaGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGtt > 1:2806627/1‑91 (MQ=255) gACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTCATAGCTATATTTTTGTATTTATGTTTTCTAGTTTaa > 2:2473552/1‑91 (MQ=255) aCAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAAc > 1:737700/1‑91 (MQ=255) cagcagTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACa > 2:2414620/1‑91 (MQ=17) agcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAt > 1:2371154/1‑91 (MQ=255) gcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt < 1:1249491/91‑1 (MQ=255) gcagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACAtt > 1:634420/1‑91 (MQ=255) cagTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATta < 1:1125318/91‑1 (MQ=255) cagTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATta > 2:1462288/1‑91 (MQ=18) agTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtac < 1:3803159/91‑1 (MQ=255) agTAAGATATTTCCAAATTGAAATTATCCTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtac > 2:4583/1‑91 (MQ=18) gcaGGATAATTTCAATTTGGAAATATCTTACTGCTGTCTTTTCTAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtact > 2:966704/3‑91 (MQ=255) gTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtact < 2:1552644/91‑1 (MQ=255) tAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtacta < 2:3118012/91‑1 (MQ=255) tAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtacta > 1:3397173/1‑91 (MQ=255) tAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATtacta > 1:1588334/1‑91 (MQ=255) ggATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTactact < 2:2065995/91‑1 (MQ=255) tAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTaa < 2:3154846/91‑1 (MQ=255) aaTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAAt > 2:3069087/1‑91 (MQ=255) aTTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATg > 1:2713195/1‑91 (MQ=255) | ACAATAGTGTGCAAGTTGGGGTGGGACGACGAAATAAATTTTGCGAAAATATCATTTCTGTCCCACTCCCAAAAAGACAGCAGTAGGATAATTTCAATTTGGAAATATCTTACTGCTGTTTTTTATAGTAATAGCTATATTTTTGTATTTATGTTTTCTAGTTTAACATTACTACTTAATG > USA300TCH1516_ALE/954678‑954858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |