| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,055,126 | T→G | 9.7% | T352T (ACA→ACC) | patA_2 ← | Putative N‑acetyl‑LL‑diaminopimelate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,055,126 | 0 | T | G | 9.7% | 854.2 / 78.6 | 278 | T352T (ACA→ACC) | patA_2 | Putative N‑acetyl‑LL‑diaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (145/105); new base G (22/5); total (168/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
Only 100 of 282 total aligned reads displayed.
ATTTAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTAATA > USA300TCH1516_ALE/1055036‑1055216 | aTTTAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCt < 1:345216/91‑1 (MQ=255) ttAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGt > 1:69750/1‑91 (MQ=255) tAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACAGAATTCGGTa < 1:1877658/91‑1 (MQ=255) aaaTATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTaa > 1:403986/1‑91 (MQ=255) aaaTATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTaa > 1:1561192/1‑91 (MQ=255) atatTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACAGAATTCGGTAAAg < 2:3659143/91‑1 (MQ=255) atagTTAGCGAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACAGAATTCGGTAAAg < 1:3549810/91‑1 (MQ=255) tatTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAgg < 2:3405386/91‑1 (MQ=255) tatTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAgg < 1:659699/91‑1 (MQ=255) tatTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAgg < 2:680442/91‑1 (MQ=255) atTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGa < 2:2706706/91‑1 (MQ=255) ttAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAAGCCTTACAAATCCTTTACCGAATTCTGTAAAGGATg < 2:2230883/91‑1 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAGACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc < 1:3694254/91‑1 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc > 1:1066409/1‑91 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc > 1:1067187/1‑91 (MQ=255) cAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGcc < 1:3811955/91‑1 (MQ=255) aaGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCt > 1:322780/1‑91 (MQ=255) aGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTg < 1:1669010/91‑1 (MQ=255) gTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTgg < 1:2012965/91‑1 (MQ=255) tctTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAAc < 2:2564081/91‑1 (MQ=255) ttCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGa > 2:1962996/1‑91 (MQ=255) cATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGAtt < 2:159052/91‑1 (MQ=255) aTACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACACATCCTATACCGAATTCTGTAAAGGATGAGCCTGGAACGAttt > 2:3915384/1‑90 (MQ=255) aTACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTg < 2:474493/91‑1 (MQ=255) tACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATGCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGc < 2:31013/91‑1 (MQ=255) tACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGc < 1:1290220/91‑1 (MQ=255) tACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGc < 2:2389802/91‑1 (MQ=255) gaCCTTCTTTTAACACGTCCATGTCAGGAGCAGAGGAAATCCTTACAAATCCTTTTCCGAATTCTGTAAAGGAGGAGCCTGGAACGATTGc < 2:1994244/90‑1 (MQ=255) aCCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCt < 1:2663593/91‑1 (MQ=255) ccTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTa > 2:2804016/1‑91 (MQ=255) tcttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAt > 1:2775685/1‑91 (MQ=255) tcttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAt < 2:2876939/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 1:2607168/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 1:1431175/91‑1 (MQ=255) cttTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtg < 2:1845139/91‑1 (MQ=255) ttttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGTATGAGCCTGGACCGATTGCTAAAtgt > 2:786041/1‑91 (MQ=255) tttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtgtg < 2:3747593/91‑1 (MQ=255) tttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCAAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAAtgtg < 2:125022/91‑1 (MQ=255) ttAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATgtgt < 2:3805458/91‑1 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGTCTGGAACGATTACTAAATGTGtt > 2:3011386/1‑91 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGtt > 1:306495/1‑91 (MQ=255) tAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGtt < 2:1657749/91‑1 (MQ=255) aaaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGa > 1:3519242/1‑91 (MQ=255) aaaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATCAGCCTGGAATGATTGCTAAATGTGTTGa > 2:195020/1‑91 (MQ=255) aaCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGAt > 2:1687481/1‑91 (MQ=255) aCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGAtt > 2:1143606/1‑91 (MQ=255) aCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGAtt > 2:3589588/1‑91 (MQ=255) aCATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGAtt < 1:2462095/91‑1 (MQ=255) cgtCCATTTCGTAAGCATAGGAAAGCGTTACAAATCGTTTACCGAATTCTGGAAAGGATGAGCCTGGAACGATTGCTAAATGTGTCGAttt < 2:1893339/89‑2 (MQ=255) cATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTc < 2:820202/91‑1 (MQ=255) tCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCaa > 1:151203/1‑91 (MQ=255) tCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCaa > 1:3302954/1‑91 (MQ=255) ccATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAg > 1:1534453/1‑91 (MQ=255) cATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGt > 1:1273019/1‑91 (MQ=255) cATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGGGTTGATTCAAGt < 1:3056030/91‑1 (MQ=255) aTTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTa > 1:794895/1‑91 (MQ=255) aTAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCg > 2:1209798/1‑91 (MQ=255) aTAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCg > 2:1536497/1‑91 (MQ=255) aTAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCg > 2:3304029/1‑91 (MQ=255) aTAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGCAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCg > 1:3426191/1‑91 (MQ=255) aaGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGac > 2:2724645/1‑91 (MQ=255) aGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGaca < 1:2370440/91‑1 (MQ=255) gCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGacac > 1:2063230/1‑91 (MQ=255) tAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACaaa < 2:3352383/91‑1 (MQ=255) tAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACaaa > 2:1353378/1‑91 (MQ=255) aGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACaaaa < 2:2789259/91‑1 (MQ=255) ggAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAAt < 2:3204736/91‑1 (MQ=255) gAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAAtt > 1:855651/1‑91 (MQ=255) tCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaa > 1:3633596/1‑91 (MQ=255) ccTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaca > 2:1488373/1‑89 (MQ=255) ccTTACAAAGCCTTTACCGAGTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGaaa < 1:1879259/91‑1 (MQ=255) cTTACAAATCCTTTACCGTATTCTGTAAAGGATGAGCCTGGAGCGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAAt < 2:26117/91‑1 (MQ=255) ttACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATc > 2:2519169/1‑91 (MQ=255) ttACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATc < 2:3826858/91‑1 (MQ=255) tACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCg > 1:1627159/1‑91 (MQ=255) aCAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGt > 1:3845023/1‑91 (MQ=255) aCAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGATAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAAtaga > 1:603007/1‑88 (MQ=255) cAAGTCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAGATGTGTTGATTCAAGTAAATCGACACAAATTTCGACATCGTc < 2:2630449/91‑1 (MQ=255) cAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAGTTCGAAATCGTc < 1:3041354/91‑1 (MQ=255) aaTCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCAt < 2:1171985/91‑1 (MQ=255) aaTCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCAt < 2:6180/91‑1 (MQ=255) aTCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAAACGACATc > 1:490617/1‑91 (MQ=255) ccTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCgg < 2:1383229/91‑1 (MQ=255) ccTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCgg > 1:3902716/1‑91 (MQ=255) cTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGt < 1:2871953/91‑1 (MQ=255) cTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGt > 1:967010/1‑91 (MQ=255) cTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGt > 1:552274/1‑91 (MQ=255) cTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGt > 1:2682520/1‑91 (MQ=255) ttACAGAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGa > 1:1770783/1‑91 (MQ=255) tacagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACACAATTCGAAATCGTCATCGGTGat > 2:3883935/5‑91 (MQ=255) acagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGACATCGTCATCGGTGata > 2:3780868/4‑91 (MQ=255) aCCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGata > 1:1808603/1‑91 (MQ=255) cagAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGatat > 1:768862/3‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 1:2116891/2‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 1:1732945/2‑91 (MQ=255) agAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATg > 2:2307562/2‑91 (MQ=255) gAATTCGGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGt > 1:2228736/1‑91 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGttt > 2:1717634/1‑91 (MQ=255) aTTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAACCCGAAATCGTGATCGGTGATATGttt > 2:3421623/1‑91 (MQ=255) tGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTAATa > 2:1104444/1‑91 (MQ=255) | ATTTAAATATTTAGCAAGTCTCTTCATACCTTCTTTTAAAACATCCATTTCATAAGCATAGGAAATCCTTACAAATCCTTTACCGAATTCTGTAAAGGATGAGCCTGGAACGATTGCTAAATGTGTTGATTCAAGTAAATCGACACAAAATTCGAAATCGTCATCGGTGATATGTTTAATA > USA300TCH1516_ALE/1055036‑1055216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |