| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,078,152 | A→T | 13.7% | Q510L (CAG→CTG) | purL → | Phosphoribosylformylglycinamidine synthase subunit PurL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,078,152 | 0 | A | T | 13.7% | 692.3 / 87.8 | 248 | Q510L (CAG→CTG) | purL | Phosphoribosylformylglycinamidine synthase subunit PurL |
| Reads supporting (aligned to +/- strand): ref base A (108/104); new base T (8/26); total (117/131) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 251 total aligned reads displayed.
ATCGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAAC > USA300TCH1516_ALE/1078062‑1078242 | aTCGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACa > 2:3722515/1‑91 (MQ=255) ctaaaTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGt < 1:1301786/89‑1 (MQ=255) gAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGtt < 2:1534232/91‑1 (MQ=255) gAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTAGGGTTCTACTGAAAAGAAAGAAATCTATCAACTGtt < 2:2723104/91‑1 (MQ=255) aaaTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTg > 2:826470/1‑91 (MQ=255) aaTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGa < 2:3554722/91‑1 (MQ=255) aaTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGa < 2:1250598/91‑1 (MQ=255) agttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGAt < 2:3444258/89‑1 (MQ=255) aTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGAt < 1:1881194/91‑1 (MQ=255) aTTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGAt > 2:3061077/1‑91 (MQ=255) aTTTAATTGCCGTGGGTGCAACACCATTAGCAAAGACCGATTGTTTCAATTATGGTTCTGCGGAAAAGAAAGAAATCTATCAACAGTTGAt > 1:3162944/1‑91 (MQ=255) tttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATa < 1:1232838/91‑1 (MQ=255) ttAATTGCCGTGGGTGCAGCACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAg < 2:2279959/91‑1 (MQ=255) ttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAg < 1:1497159/91‑1 (MQ=255) ttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAg < 1:3860731/91‑1 (MQ=255) ttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAg < 1:1935503/91‑1 (MQ=255) ttAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAg < 2:3592612/91‑1 (MQ=255) tAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAGa < 2:3254300/91‑1 (MQ=255) tAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGa > 1:3693459/1‑91 (MQ=255) tAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGa < 1:589699/91‑1 (MQ=255) aaTTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAGAGAAAGAAATCTATCAACAGTTGATAGAt < 2:2507863/91‑1 (MQ=255) aaTTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAGAt < 1:2842277/91‑1 (MQ=255) aaTTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGAt < 2:3800806/91‑1 (MQ=255) aTTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGAtt < 1:3012567/91‑1 (MQ=255) tGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAGAtttc < 1:2233853/91‑3 (MQ=255) tGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAGAtttc < 2:3208056/91‑3 (MQ=255) ccGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACTGTTGATAGAtttctt < 1:2645762/91‑5 (MQ=255) ccGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAAc > 1:2504415/1‑91 (MQ=255) ccGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAAc > 1:2519541/1‑91 (MQ=255) ccGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAAc < 1:730752/91‑1 (MQ=255) ccGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTACCAACAGTTGATAGATTCAAc < 1:2727170/91‑1 (MQ=255) cGTGGGTGCAAGATCATTAGCAATGACAGGTTGTTTAAATTATGGTTCTCCTGTAAAGAAAGAAATCTATCAACAGTTGATATATTCAACg < 2:3153396/91‑1 (MQ=255) cGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACg < 1:1993713/91‑1 (MQ=255) cGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACg > 1:3558744/1‑91 (MQ=255) gTGGGTGCGACACCATTAGCAATGACAGATTGTGTAAATTATGGTTCTCCTGAGAGGAAAGAAATCTATCAACCGTTGAGAGATTCAACGa < 2:1024424/91‑1 (MQ=255) gTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGa < 1:3638339/91‑1 (MQ=255) tGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGaa < 1:3747381/91‑1 (MQ=255) gggTGCAACACCATTAGCAATGGCAGATTGCTTAATTGATGGTTCTCCTGACAAGAAAGTAATCTATCAACCGTTGATAGATTCAACGaaa > 2:1213372/1‑91 (MQ=255) gggTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGaaa > 2:3693700/1‑91 (MQ=255) gTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTGAACGAAAgg > 1:3547751/1‑91 (MQ=255) gTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAgg < 2:487879/91‑1 (MQ=255) tGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGt > 2:2021092/1‑91 (MQ=255) gCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTa < 1:2009491/91‑1 (MQ=255) aaCACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATg > 2:3590706/1‑91 (MQ=255) aaCACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATg > 2:2442549/1‑91 (MQ=255) acacCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATgg > 2:2337151/1‑91 (MQ=255) acacCATTAGCAATGACAGATCGCTTAAAATATGGTCCTCCTGAAAAGAAAGAAATCTATCAACAGCTCATAGATTCAACGAAAGGTAtcg > 2:3264159/1‑89 (MQ=255) acCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCa < 2:1129263/91‑1 (MQ=255) acCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCa < 2:3627945/91‑1 (MQ=255) ccATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAg > 1:273942/1‑91 (MQ=255) cATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGa > 1:617565/1‑91 (MQ=255) tAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGc > 2:784323/1‑91 (MQ=255) tAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGc > 2:2166576/1‑91 (MQ=255) aGCAATGACTGACTGCTTAAATTATGGTTCTCCTGAAAAGAAAGAAATTTATCAACAGCTGATAGATTCAACGAAGGGTATGGCAGAAGCa < 1:2388245/91‑1 (MQ=255) aGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCa > 1:3224572/1‑91 (MQ=255) gCAATGACAGATTGTTTAAATTATTGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCAt < 2:3674885/91‑1 (MQ=255) aaTGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGc < 1:3911371/91‑1 (MQ=255) aTGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCg < 2:3851517/91‑1 (MQ=255) aTGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCg < 2:1653116/91‑1 (MQ=255) tGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGa < 1:1802547/91‑1 (MQ=255) gACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGAc < 2:1969642/91‑1 (MQ=255) aCAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACa < 2:2096633/91‑1 (MQ=255) gaGATGGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGAGAGATTCAACGAAAGGTATGGCAGAAGCATGCGACAt < 2:1327091/90‑1 (MQ=255) cAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACAt > 2:2866882/1‑91 (MQ=255) aTTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCt > 2:1891797/1‑91 (MQ=255) aTTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCt > 1:3818238/1‑91 (MQ=255) ttGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCtt > 1:1051196/1‑91 (MQ=255) ttAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGa > 1:937151/1‑91 (MQ=255) ttAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGa > 1:1778840/1‑91 (MQ=255) ttAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGa > 1:3256118/1‑91 (MQ=255) aaaTTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGaca < 2:1221681/91‑1 (MQ=255) aTTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACAcc > 2:675857/1‑91 (MQ=255) tagggTTCTCCGGAAAAAAAAGAAATCGATCAACAGTTGATAGGTTCAACGAAGGGTATGGCAGAAGCATGCGACATTCTTAAGACACCag < 2:1465691/88‑1 (MQ=255) tATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCag < 1:1116641/91‑1 (MQ=255) tATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCag < 1:3000710/91‑1 (MQ=255) aTGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCagt < 1:2314726/91‑1 (MQ=255) tGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCagta > 1:1442866/1‑91 (MQ=255) ggTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCagtag > 2:3908964/1‑91 (MQ=255) ggTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCagtag > 1:1515785/1‑91 (MQ=255) ttCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGtt < 1:2098947/91‑1 (MQ=255) tctcCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTCAAGACACCAGTAGttt > 2:3784883/1‑91 (MQ=255) ctcCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTc > 1:3805201/1‑91 (MQ=255) tGAGAAGAAAGAAATCGAACAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGt < 2:3893625/91‑1 (MQ=255) tGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGt < 1:3370283/91‑1 (MQ=255) gAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTa < 1:1634544/91‑1 (MQ=255) gAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTa > 2:1164263/1‑91 (MQ=255) gAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTa < 1:1369958/91‑1 (MQ=255) aaaaGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTaa < 2:2519813/91‑1 (MQ=255) agaaagaaATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGt < 2:1777224/91‑1 (MQ=255) aagaaaTCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATc < 1:2871153/91‑1 (MQ=255) aTCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTtat < 2:3418129/91‑1 (MQ=255) aTCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTtat > 1:1194268/1‑91 (MQ=255) tATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTtatata > 1:2203772/1‑91 (MQ=255) aTCAACTGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaa > 2:1457013/1‑91 (MQ=255) aTCAACTGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaa > 1:2085261/1‑91 (MQ=255) aTCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaa < 2:245687/91‑1 (MQ=255) tCAACTGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACACTCTTAAGACACCAGTAGTTTCTGGTAATGCATCTTTATATaac > 1:3955386/1‑91 (MQ=255) aaCAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaacga > 2:3048562/1‑91 (MQ=255) aCAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaacgaa > 1:2608758/1‑91 (MQ=255) aGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATaacgaaac < 2:574219/91‑1 (MQ=255) | ATCGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGTTCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAAGCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAAC > USA300TCH1516_ALE/1078062‑1078242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |