| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,158,435 | C→G | 9.6% | intergenic (+19/‑305) | uvrC → / → sdhC | UvrABC system protein C/Succinate dehydrogenase cytochrome b558 subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,158,435 | 0 | C | G | 9.6% | 750.1 / 53.4 | 228 | intergenic (+19/‑305) | uvrC/sdhC | UvrABC system protein C/Succinate dehydrogenase cytochrome b558 subunit |
| Reads supporting (aligned to +/- strand): ref base C (106/100); new base G (7/15); total (113/115) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
Only 100 of 234 total aligned reads displayed.
CTTGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTCTAAATGA > USA300TCH1516_ALE/1158345‑1158525 | cTTGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATc > 1:1126907/1‑91 (MQ=255)cTTGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATc > 2:3830005/1‑91 (MQ=255)cTTGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATc < 2:1948464/91‑1 (MQ=255) tGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTGGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATGAt < 2:3112300/91‑1 (MQ=255) gAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCATGAGAATGAtt < 2:647649/91‑1 (MQ=255) gAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCATGAGAATGAtt < 1:557474/91‑1 (MQ=255) aaGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATGATtc < 1:3288185/91‑1 (MQ=255) aaGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCATGAGAATGATtc < 2:2986373/91‑1 (MQ=255) aGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATtct < 1:2416606/91‑1 (MQ=255) aTTTTAAAAATATAGGTATTCCTGACACCGTCGCAAAGAACCCACATGAACAATTGCATCAATAAATTACTGGGCGTGAGActcctcctcc > 2:3743756/1‑90 (MQ=255) tttAAAAATATTGGTATTCCTGAAAACGTCGCAAAGAACCTTCATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATg < 1:408798/91‑1 (MQ=255) ttAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGc > 1:3770176/1‑91 (MQ=255) ttAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGc < 1:1946672/91‑1 (MQ=255) ttAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGc > 1:2825097/1‑91 (MQ=255) ggaaaaaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATGGCATAAATAAATTACTGGGCATGAGAATGATTCTCACGc < 2:20122/89‑1 (MQ=255) aaaaaTATAGGTATTCCTGAAAACGTCGCAAAGATCCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGccc < 2:765763/91‑1 (MQ=255) aaaaaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGccc > 1:1744087/1‑91 (MQ=255) aaaaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGcccc < 1:993088/91‑1 (MQ=255) aaaaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGcccc < 2:1500696/91‑1 (MQ=255) aaaaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGcccc > 1:3831665/1‑91 (MQ=255) aaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCaa < 1:833195/91‑1 (MQ=255) aaTATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCaa > 1:1383629/1‑91 (MQ=255) atatAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCaaa > 2:3917654/1‑91 (MQ=255) tataGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCaaaa < 2:2589933/91‑1 (MQ=255) aTTCCTGAAAACGTCGCAGAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGa < 1:860066/91‑1 (MQ=255) aTTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGa < 2:2771631/91‑1 (MQ=255) ttCCGGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGaa < 1:3665485/91‑1 (MQ=255) ccTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaat > 2:311453/1‑91 (MQ=255) cTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaata > 1:3905468/1‑91 (MQ=255) tGAAATCGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaataa < 2:3644389/91‑1 (MQ=255) tGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaataa < 2:2639174/91‑1 (MQ=255) tGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaataa > 1:2915120/1‑91 (MQ=255) gAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAaataat > 2:3333387/1‑91 (MQ=255) aaaaCGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATCACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATg > 2:3052634/1‑91 (MQ=255) aaCGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGtt < 1:3232298/91‑1 (MQ=255) aCGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTa > 2:3011310/1‑91 (MQ=255) cGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTAc > 1:2325989/1‑91 (MQ=255) tCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACaa > 1:2935832/1‑91 (MQ=255) cGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAat > 1:3109301/1‑91 (MQ=255) cGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAat > 2:2557016/1‑91 (MQ=255) cGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAat > 2:3713536/1‑91 (MQ=255) cGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAat > 2:860216/1‑91 (MQ=255) gCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAata > 2:3232116/1‑91 (MQ=255) aaaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATg < 2:3536040/91‑1 (MQ=255) aaaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATg < 2:3169410/91‑1 (MQ=255) aaaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATg < 2:1442583/91‑1 (MQ=255) aaaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATg < 1:1841734/91‑1 (MQ=255) aaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATgg > 2:2298884/1‑91 (MQ=255) aaGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATgg > 2:1243659/1‑91 (MQ=255) aGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGt > 2:1665923/1‑91 (MQ=255) aGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGt < 2:2843159/91‑1 (MQ=255) aCCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTaa > 1:39234/1‑91 (MQ=255) ccTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAAc > 2:503545/1‑91 (MQ=255) cTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACa > 2:628464/1‑91 (MQ=255) tACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACaa > 2:2392225/1‑91 (MQ=255) aTGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGt < 2:2201330/91‑1 (MQ=255) aTGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGt < 2:345523/91‑1 (MQ=255) aTGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGt < 1:1003962/91‑1 (MQ=255) aTGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGt < 2:2784554/91‑1 (MQ=255) aTGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATGCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGt < 2:1303583/91‑1 (MQ=255) tGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGtt > 2:1001970/1‑91 (MQ=255) gAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTa > 1:2647212/1‑91 (MQ=255) aaCAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAt < 2:169607/91‑1 (MQ=255) aaCAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAt > 1:2176290/1‑91 (MQ=255) aaCAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAt > 1:1614028/1‑91 (MQ=255) cAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAttt > 2:2925515/1‑91 (MQ=255) cAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAttt < 1:635784/91‑1 (MQ=255) cAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAttt > 2:3599701/1‑91 (MQ=255) ttGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAtttttt > 2:2798882/1‑91 (MQ=255) ttGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAtttttt < 2:3368461/91‑1 (MQ=255) tGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTAttttttt > 2:306594/1‑91 (MQ=255) cATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGa > 2:1221051/1‑91 (MQ=255) ataaataaATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGaa > 2:1475604/1‑91 (MQ=255) ataaataaATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTGTGaa < 1:3122542/91‑1 (MQ=255) aaataaatTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATg < 2:1122000/91‑1 (MQ=255) aataaatTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGt > 1:2859773/1‑91 (MQ=255) taaatTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTAc > 1:1158935/1‑91 (MQ=255) taaatTACTGAGCGTGTGATTCATTCTCATGATCCGTATTATATGAAATAAGGTTACGATATGGTTAACAAAGTTATTTTTGGGAATGTAc < 2:454356/91‑1 (MQ=255) atTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTg > 1:2429481/1‑91 (MQ=255) atTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTg > 1:2538758/1‑91 (MQ=255) atTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTg > 1:3229858/1‑91 (MQ=255) ttACTGGGCGTGAGAATCATTCTCATGCCCCAGAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTCTTTGAATGTACGTgg < 1:3367695/91‑1 (MQ=255) ttACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTgg < 1:2781663/91‑1 (MQ=255) ttACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTgg > 1:3602192/1‑91 (MQ=255) ttggggCATGAGAATGATTCTCACGCCCCAATATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTCGAATGTACGTAGaa > 1:3564139/4‑91 (MQ=25) ttggggCATGAGAATGATTCTCACGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGaa > 1:3286116/4‑91 (MQ=38) aCTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGaa < 2:2112858/91‑1 (MQ=255) cTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAg > 2:1875376/1‑91 (MQ=255) tGGGCGTGAGAATCATTCTCATGTCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGc > 2:1117260/1‑91 (MQ=255) ggggCATGAGAATGATTCTCACGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGc > 1:765977/2‑91 (MQ=38) ggggCATGAGAATGATTCTCACGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGc > 2:1750655/2‑91 (MQ=38) gggCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCg < 1:1611440/91‑1 (MQ=255) gggCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCg < 1:1431779/91‑1 (MQ=255) ggCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGt < 2:1011617/91‑1 (MQ=255) cGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGttt < 1:2998447/91‑1 (MQ=255) tGAGAATGATTCTCACGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTc > 1:2735648/1‑91 (MQ=255) gaATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTCTaa < 2:966542/91‑1 (MQ=255) aTCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTCAACAAAGTTATTTTTTTGAATGTACGTGGCAGCGTTTTCTAAAt > 2:3399303/1‑91 (MQ=255) tCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTCTAAATg > 1:593146/1‑91 (MQ=255) tCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTCTAAATg < 1:14474/91‑1 (MQ=255) | CTTGAAGATTTTAAAAATATAGGTATTCCTGAAAACGTCGCAAAGAACCTACATGAACAATTGCATAAATAAATTACTGGGCGTGAGAATCATTCTCATGCCCCAAAATATATGAAATAATGTTACAATATGGTTAACAAAGTTATTTTTTTGAATGTACGTGGAAGCGTTTTCTAAATGA > USA300TCH1516_ALE/1158345‑1158525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |