| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,447,045 | T→A | 6.3% | I64F (ATC→TTC) | cobT ← | Aerobic cobaltochelatase subunit CobT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,447,045 | 0 | T | A | 6.3% | 844.2 / 33.3 | 252 | I64F (ATC→TTC) | cobT | Aerobic cobaltochelatase subunit CobT |
| Reads supporting (aligned to +/- strand): ref base T (121/114); new base A (13/3); total (135/117) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
Only 100 of 263 total aligned reads displayed.
CTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTTGTG > USA300TCH1516_ALE/1446955‑1447134 | cTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGat < 1:2579463/91‑1 (MQ=255) ttGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGata < 1:2840454/91‑1 (MQ=255) tGTACTACTTCATTTACAATTTGTACATCCATCATCTGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATATCGaaat < 2:2753798/91‑1 (MQ=255) gTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATg > 2:325082/1‑91 (MQ=255) gTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATATCGAAATg < 2:445184/91‑1 (MQ=255) tactacTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATgg < 2:3262250/91‑1 (MQ=255) actactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGc < 1:3707086/91‑1 (MQ=255) actactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGc > 1:872185/1‑91 (MQ=255) actactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGc < 2:2000554/91‑1 (MQ=255) ctactTCATTTACGATTTGTACATCCATCATATGATAACCGTAGGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCt < 1:3949280/91‑1 (MQ=255) ctactGCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCt < 2:1822956/91‑1 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCtt > 2:144714/1‑91 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCtt < 1:202000/91‑1 (MQ=255) tactTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCtt > 2:2836639/1‑91 (MQ=255) actTCATTTACAATTTGTACATCCATCATATGATAACCGTCAGCAGCTAACATAACATCTGTTCTTGGACCAGCCATTTCCATATGGCTTg > 2:3667987/1‑91 (MQ=255) ttCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGt < 1:1009413/91‑1 (MQ=255) tCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTc < 1:737888/91‑1 (MQ=255) tCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTc > 1:1362690/1‑91 (MQ=255) cATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCg > 2:2451902/1‑91 (MQ=255) tttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTcaca < 2:1793696/91‑4 (MQ=255) ttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtg > 2:3751559/1‑91 (MQ=255) ttACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtg < 1:2756624/91‑1 (MQ=255) tACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtgt < 1:2930687/91‑1 (MQ=255) tACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGAtgt < 2:162991/91‑1 (MQ=255) cAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGa > 2:2042045/1‑91 (MQ=255) aaTTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGAc < 2:3531048/91‑1 (MQ=255) tttGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCa < 1:375280/91‑1 (MQ=255) ttGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaa < 1:2808193/91‑1 (MQ=255) tGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaa < 1:84742/91‑1 (MQ=255) gTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCaaaa < 2:1474137/91‑1 (MQ=255) tACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAAc > 1:2213845/1‑91 (MQ=255) aCATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAAcc > 1:2457159/1‑91 (MQ=255) aCATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAAcc < 1:1716539/91‑1 (MQ=255) catccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCa < 1:1918498/91‑1 (MQ=255) tccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAAc > 1:687668/1‑91 (MQ=255) ccatcATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACt > 1:1311106/1‑91 (MQ=255) ccatcACATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACt > 1:3069478/1‑91 (MQ=255) atcatATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCg > 2:252457/1‑91 (MQ=255) atcatATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCg < 1:3398736/91‑1 (MQ=255) tcatATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATATGACCAAAACCAACTCGt > 2:2677311/1‑91 (MQ=255) catATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTa > 2:3561486/1‑91 (MQ=255) atGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATc > 1:866839/1‑91 (MQ=255) atGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATc < 2:400253/91‑1 (MQ=255) atGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATc > 2:2967262/1‑91 (MQ=255) tGATAACCGTAAGCAGCTAACATAACACCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCa < 2:2819573/91‑1 (MQ=255) aTAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaaa > 1:155133/1‑91 (MQ=255) tAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCaaaa > 2:963725/1‑91 (MQ=255) aaCCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAAc < 2:2562595/91‑1 (MQ=255) ccGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAt < 1:3197165/91‑1 (MQ=255) cGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACAtt > 2:745077/1‑91 (MQ=255) agcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGa > 1:1325586/1‑91 (MQ=255) agcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGa > 1:1982675/1‑91 (MQ=255) agcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGa < 1:2166948/91‑1 (MQ=255) agcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGa > 1:975886/1‑91 (MQ=255) gcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCACAACCAACTCGTAATCAACACATTTTgca > 2:2620868/1‑89 (MQ=255) gcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGaa < 1:1792592/91‑1 (MQ=255) gcagcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGaa > 2:2253296/1‑91 (MQ=255) agcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACa < 1:3717352/91‑1 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg > 1:573482/1‑91 (MQ=255) gcTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAg < 1:276596/91‑1 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa < 1:485212/91‑1 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa < 2:1859994/91‑1 (MQ=255) taacataacaTCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGa > 1:3018842/1‑91 (MQ=255) aacataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGAt > 1:2677834/1‑91 (MQ=255) acataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGAtt > 2:800832/1‑91 (MQ=255) acataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGAtt > 1:1245488/1‑91 (MQ=255) cataacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATta < 1:2720184/91‑1 (MQ=255) aacatCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAg > 2:3838444/1‑91 (MQ=255) catCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTa < 1:2309958/91‑1 (MQ=255) catCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTa > 1:1574442/1‑91 (MQ=255) tCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAg < 1:761524/91‑1 (MQ=255) tCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGGCGATGTGACCAAAACCAACTCGTAATCAAAACAGTTTGAACAGGATTATAGTAAg < 1:1838918/91‑1 (MQ=255) tCTATTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCACCTCGTAATCAACACATTTTGAACAGGACTATAGTAAg > 2:3505231/1‑91 (MQ=255) cTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAgg > 2:3630659/1‑91 (MQ=255) tGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCCAAACCAACTCGTAATCAAAACCTTTTGAACAGGATTATAGTAAggg > 2:441454/1‑91 (MQ=255) tGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAggg < 2:1298368/91‑1 (MQ=255) ttttAAACCAGCCATTTCGATATGGCTTGATCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGAAAGGGAAt > 2:3438169/1‑91 (MQ=255) tttAAACCAGCCATTTCGATATGTATTGGTCGATGTGACCAAAACCAACTTGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAAtt < 2:3679528/91‑1 (MQ=255) ttAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAAttt < 1:148328/91‑1 (MQ=255) tAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAAtttt < 2:2299721/91‑1 (MQ=255) aaCCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTg < 1:1686204/91‑1 (MQ=255) aCCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACCTTTTGAACAGGATTATAGTAAGGGAATTTTTgg > 2:300799/1‑91 (MQ=255) aGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAtt < 1:2400591/91‑1 (MQ=255) aGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAtt < 1:2133609/91‑1 (MQ=255) aGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGAtt < 1:1146556/91‑1 (MQ=255) catatCGAAATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTa > 2:3117453/5‑91 (MQ=255) aTTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTaa < 2:2873574/91‑1 (MQ=255) aTTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTaa > 1:1132750/1‑91 (MQ=255) tttCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAAc > 2:3411115/1‑91 (MQ=255) tatCGAAATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGCTTATAGTAAGGGAATTTTTGGATTTTAAc > 2:1056237/3‑91 (MQ=255) tatCGAAATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGCTTTTAAc > 1:344669/3‑91 (MQ=255) ttCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACt < 1:2197751/91‑1 (MQ=255) tCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACtt < 1:1537128/91‑1 (MQ=255) tCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACtt < 1:1093454/91‑1 (MQ=255) tCGAAATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACtt > 1:3307695/1‑91 (MQ=255) gATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTtgt > 1:2474598/1‑91 (MQ=255) gATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACACTTTGAACAGGATTATAGTAAGGGACTTTTTGGATTTTAACTtgt > 1:3143713/1‑91 (MQ=255) atatGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTtgtg < 1:3793776/91‑1 (MQ=255) atatGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTtgtg > 2:639393/1‑91 (MQ=255) atatGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTtgtg < 1:3793821/91‑1 (MQ=255) | CTTGTACTACTTCATTTACAATTTGTACATCCATCATATGATAACCGTAAGCAGCTAACATAACATCTGTTTTTAAACCAGCCATTTCGATATGGCTTGGTCGATGTGACCAAAACCAACTCGTAATCAAAACATTTTGAACAGGATTATAGTAAGGGAATTTTTGGATTTTAACTTGTG > USA300TCH1516_ALE/1446955‑1447134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |