Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,622,775 A→T 5.3% K43* (AAA→TAA)  marA → Multiple antibiotic resistance protein MarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,622,7750AT 5.3% 680.6 / 19.0 201K43* (AAA→TAA) marAMultiple antibiotic resistance protein MarA
Reads supporting (aligned to +/- strand):  ref base A (96/94);  new base T (6/5);  total (102/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TATATTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTACACGA  >  USA300TCH1516_ALE/1622685‑1622864
                                                                                          |                                                                                         
 atatTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTaa                                                                                          <  1:2276692/91‑1 (MQ=255)
  tatTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCCTACCATCTTGATCAATCATTTaaa                                                                                         >  2:591545/1‑91 (MQ=255)
  tatTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTtaa                                                                                         <  1:3474156/91‑1 (MQ=255)
  tatTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTaaa                                                                                         >  2:1634947/1‑91 (MQ=255)
   atTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTtaaa                                                                                        <  1:294615/91‑1 (MQ=255)
   atTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTtaaa                                                                                        <  1:1477318/91‑1 (MQ=255)
   atTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTtaaa                                                                                        <  1:3634053/91‑1 (MQ=255)
    ttGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAAt                                                                                       <  2:2999959/91‑1 (MQ=255)
    ttGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAAt                                                                                       <  2:2514437/91‑1 (MQ=255)
    ttGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAAt                                                                                       <  2:1212530/91‑1 (MQ=255)
    ttGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGA‑CAATCATTTTAAATg                                                                                      <  1:428756/91‑1 (MQ=255)
     tGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATg                                                                                      <  2:3467823/91‑1 (MQ=255)
       aaGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGAt                                                                                    <  2:2786946/91‑1 (MQ=255)
        aGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGAtt                                                                                   >  2:1722443/1‑91 (MQ=255)
        aGATCGTGGATGAGCGCCTTGCAAGTTGCAAGGAGTAAGTGATGACGTTGGTCTGTCGCCATACCATCTTGATCAATCATTTTAAATGAtt                                                                                   <  2:57077/91‑1 (MQ=255)
          aTCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGt                                                                                 >  1:2027940/1‑91 (MQ=255)
          aTCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGt                                                                                 >  1:2554102/1‑91 (MQ=255)
           tCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTc                                                                                <  2:1946073/91‑1 (MQ=255)
             gTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCgg                                                                              >  2:3695667/1‑91 (MQ=255)
              tttATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGACCAATCACAATACCACCTTGACCCATCATATAACATGATTGTCAGc                                                                             <  2:1575307/91‑1 (MQ=255)
              gttatgaGAGCCTTTCAATTTGCCAGCCTTGTGTGATTACGTTGGTCGTTCGCCATACCCCCGTGCCCACTCATTGACAACGATTGTCGGc                                                                             >  2:3227142/2‑91 (MQ=255)
                tattaGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCtt                                                                           >  2:2835612/1‑91 (MQ=255)
                 attaGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTa                                                                          <  1:2264345/91‑1 (MQ=255)
                 attaGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTa                                                                          >  2:2128466/1‑91 (MQ=255)
                  ttaGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTAt                                                                         >  1:2262822/1‑91 (MQ=255)
                  ttaGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTAt                                                                         >  1:2884455/1‑91 (MQ=255)
                   taGAGCCTTTCATTTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTAtc                                                                        <  2:3292048/91‑1 (MQ=255)
                      agCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTcc                                                                     <  2:2496566/91‑1 (MQ=255)
                      agCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTcc                                                                     >  2:1397420/1‑91 (MQ=255)
                       gCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCa                                                                    >  2:562401/1‑91 (MQ=255)
                       gCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCa                                                                    >  1:1177268/1‑91 (MQ=255)
                         cTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGa                                                                  <  2:419801/91‑1 (MQ=255)
                         cTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGa                                                                  <  1:1543427/91‑1 (MQ=255)
                            tCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGc                                                               <  1:478569/91‑1 (MQ=255)
                             cAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCt                                                              <  2:3914584/91‑1 (MQ=255)
                             cAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCt                                                              <  1:929308/91‑1 (MQ=255)
                              aaTTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCtt                                                             >  1:3815627/1‑91 (MQ=255)
                               aTTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTa                                                            <  2:2436547/91‑1 (MQ=255)
                               aTTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAAGCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTa                                                            <  1:3342541/91‑1 (MQ=255)
                                 ttGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATg                                                          >  2:2561003/1‑91 (MQ=255)
                                 ttGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATg                                                          <  1:2386001/91‑1 (MQ=255)
                                 ttGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATg                                                          >  1:483035/1‑91 (MQ=255)
                                 ttGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATg                                                          <  1:18870/91‑1 (MQ=255)
                                     aaGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcg                                                      >  1:2182939/1‑91 (MQ=255)
                                       gAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcg                                                    <  2:1320055/91‑1 (MQ=255)
                                       gAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcg                                                    >  2:1096587/1‑91 (MQ=255)
                                       gAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcg                                                    <  2:1878282/91‑1 (MQ=255)
                                        aaTTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcgc                                                   >  1:211882/1‑91 (MQ=255)
                                        aaTTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcgc                                                   >  1:2573801/1‑91 (MQ=255)
                                          ttAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCAcgcgcgc                                                 >  1:739267/1‑91 (MQ=255)
                                             aGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTa                                              >  1:3000823/1‑91 (MQ=255)
                                              gTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTaa                                             >  1:2303813/1‑91 (MQ=255)
                                                gATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTaaaa                                           >  2:1510019/1‑91 (MQ=255)
                                                gATTACGTTGGTCTTTCGCCATACCATCTGGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTaaaa                                           <  1:2086626/91‑1 (MQ=255)
                                                  ttACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATg                                         >  2:1521756/1‑91 (MQ=255)
                                                  ttACGTTGGTCTTTCGCCATACCATCGTGATCAATCATTGAAAATGATTGTCGGCTTATCGCCAGAGGCTTGTGCACGCGCGCGTAAAATg                                         <  2:2720066/91‑1 (MQ=255)
                                                   tACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGa                                        >  1:1071987/1‑91 (MQ=255)
                                                    aCGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGac                                       <  2:2732829/91‑1 (MQ=255)
                                                       ttGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGAGTGTCGGCTTATCTCCAGAAGCTTATGCACGCTCGCGTAAAATGACGCt                                    <  2:3531482/91‑1 (MQ=255)
                                                         ggTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCg                                  >  1:3152543/1‑91 (MQ=255)
                                                          gTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGc                                 >  1:2323677/1‑91 (MQ=255)
                                                          gTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGc                                 >  1:1347642/1‑91 (MQ=255)
                                                          gTCTTTCGCATTACCTTTTTAATCAATCATTTAAAAGGATGGTCGGTTTATCTCCGAAAGCTTAGGCACGCGCGCGTAAAATGACACTCGc                                 <  2:3814593/91‑1 (MQ=255)
                                                            cTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTg                               <  1:3797846/91‑1 (MQ=255)
                                                             tttCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGc                              >  2:1850075/1‑91 (MQ=255)
                                                             tttCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGc                              <  2:2688961/91‑1 (MQ=255)
                                                               tCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAATATGACACTCGCTGCaa                            >  2:142016/1‑91 (MQ=255)
                                                               tCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCaa                            >  2:639056/1‑91 (MQ=255)
                                                               tCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCaa                            <  1:1443238/91‑1 (MQ=255)
                                                                 gCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAat                          >  2:71352/1‑91 (MQ=255)
                                                                   cATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAatga                        >  1:1327600/1‑91 (MQ=255)
                                                                    aTACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAatgat                       <  1:874858/91‑1 (MQ=255)
                                                                     tACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAatgatg                      >  1:691321/1‑91 (MQ=255)
                                                                     tACCAGCTTGATCAATCATTTAAAATGATTGTCGGCGTATCGCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAatgatg                      <  2:2635314/91‑1 (MQ=255)
                                                                      aCCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAGGCTTATGCACGCGCGCGTAAAATGACAATCGCTGCCAatgatgg                     >  2:3774233/1‑90 (MQ=255)
                                                                       ccATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGAtgtg                    >  2:2863744/1‑91 (MQ=255)
                                                                        cATCTTGATCAATGATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACAGTCGCTGCAAATGATGTGa                   <  2:3525944/91‑1 (MQ=255)
                                                                        cATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGa                   <  1:708597/91‑1 (MQ=255)
                                                                         aTCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGAt                  >  2:765786/1‑91 (MQ=255)
                                                                          tCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAGTGACACTCGCTGCAAATGATGTGAtt                 <  2:2236165/91‑1 (MQ=255)
                                                                          tCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGAtt                 <  2:3610909/91‑1 (MQ=255)
                                                                          tCTTGATCAATCATTTAAAATGATCGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGAtt                 <  2:3671291/91‑1 (MQ=255)
                                                                           cTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTa                <  1:3504294/91‑1 (MQ=255)
                                                                           cTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTa                >  1:1274434/1‑91 (MQ=255)
                                                                             tGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAAt              <  2:1770707/91‑1 (MQ=255)
                                                                             tGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAAt              <  1:2622768/91‑1 (MQ=255)
                                                                             tGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAAt              <  1:3768508/91‑1 (MQ=255)
                                                                                 caatcaTTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTg          >  2:235568/1‑91 (MQ=255)
                                                                                  aatcaTTTAATATCATTGTCGGCTTAGCTCCAGAAGCTTATGCGCGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGc         <  1:3953146/91‑1 (MQ=255)
                                                                                  aatcaTTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGc         >  2:590381/1‑91 (MQ=255)
                                                                                    tcaTTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTa       <  1:277049/91‑1 (MQ=255)
                                                                                     caTTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTac      >  1:3511909/1‑91 (MQ=255)
                                                                                      aTTTTAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTaca     >  2:939422/1‑91 (MQ=255)
                                                                                      aTTTTAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTaca     >  1:3606607/1‑91 (MQ=255)
                                                                                      aTTTTAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTaca     >  1:1758484/1‑91 (MQ=255)
                                                                                      aTTTTAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTaca     >  1:1133658/1‑91 (MQ=255)
                                                                                      aTTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTaca     <  1:1664919/91‑1 (MQ=255)
                                                                                       tttAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTacac    <  2:2031998/91‑1 (MQ=255)
                                                                                       tttAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTacac    >  2:2017600/1‑91 (MQ=255)
                                                                                         tAAAATGACTGTCGGCTTATCTCCATGAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTACACGa  <  2:3426938/91‑1 (MQ=255)
                                                                                          |                                                                                         
TATATTGAAGATCGTTTATTAGAGCCTTTCAATTTGCAAGAATTAAGTGATTACGTTGGTCTTTCGCCATACCATCTTGATCAATCATTTAAAATGATTGTCGGCTTATCTCCAGAAGCTTATGCACGCGCGCGTAAAATGACACTCGCTGCAAATGATGTGATTAATGGTGCTACACGA  >  USA300TCH1516_ALE/1622685‑1622864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑