| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,286,337 | C→T | 5.9% | intergenic (‑250/+27) | ebh_4 ← / ← glmM | Extracellular matrix‑binding protein ebh/Phosphoglucosamine mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,286,337 | 0 | C | T | 5.9% | 741.9 / 22.0 | 230 | intergenic (‑250/+27) | ebh_4/glmM | Extracellular matrix‑binding protein ebh/Phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base C (97/118); new base T (2/12); total (100/130) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
Only 100 of 234 total aligned reads displayed.
TACATATTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAGCA > USA300TCH1516_ALE/2286247‑2286427 | cATATTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGc < 2:2472169/91‑1 (MQ=255) atagTGATTACTTTGTTGTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAAAAAAAAAAACCGATGCATACGCa < 2:2818393/91‑1 (MQ=255) tatTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCGTATGCAt < 1:452469/91‑1 (MQ=255) tatTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCGTATGCAt < 1:3719877/91‑1 (MQ=255) tatTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCGTATGCAt < 1:1701187/91‑1 (MQ=255) ttGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCGTATGCATCg < 1:1523666/91‑1 (MQ=255) ttGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCGTATGCATCg < 2:3070174/91‑1 (MQ=255) tGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCgg < 1:881719/91‑1 (MQ=255) tGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCgg < 1:3919159/91‑1 (MQ=255) tGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCgg < 2:1872211/91‑1 (MQ=255) tGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCgg < 1:360925/91‑1 (MQ=255) tGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCgg > 2:2719975/1‑91 (MQ=255) tGATTACTTTGTTTTTATTTTGTGTATATCATTTTACGTTTGTACTATAAATTATTTCTACCAACACAATAAACTGATGCACACGCATCgg > 2:2717032/1‑91 (MQ=255) gATTACTTTGTTTTTATTTTGTTGATATCATTTTACGTTTGTACTATAGATTATTTCTACAAACACAAAAAACCGATGCGTATGCATCggt < 2:155459/91‑2 (MQ=255) aTTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCt < 1:1444463/91‑1 (MQ=255) aTTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGGACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCt < 2:1985989/91‑1 (MQ=255) cTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCAtt < 1:2697544/91‑1 (MQ=255) ttcttttcATGTTGATTATCTCATATGACGTTTGCACTATAGTTTGCGTAGAATAACACAAAAAACCGATGCATACGCATCGGCTCATTTg < 2:3830966/88‑1 (MQ=255) ttGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTg > 2:2156609/1‑91 (MQ=255) tGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGt < 1:1112960/91‑1 (MQ=255) tGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGt > 1:2960785/1‑91 (MQ=255) tGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGt < 1:2433233/91‑1 (MQ=255) tttttATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTaa > 1:3630759/1‑91 (MQ=255) tttttATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTaa < 1:3446932/91‑1 (MQ=255) tttttATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACAAAAAAAACCGATGCATACGCATCGGCTCATTTGTaa < 2:3328189/91‑1 (MQ=255) ttttATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAAt < 1:902168/91‑1 (MQ=255) ttttGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGt > 1:3765307/1‑91 (MQ=255) tttGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGta < 1:936012/91‑1 (MQ=255) ttGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtat > 1:2749678/1‑91 (MQ=255) ttGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtat < 1:1657039/91‑1 (MQ=255) tGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtatt > 1:3077613/1‑91 (MQ=255) tGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtatt > 2:2592837/1‑91 (MQ=255) tGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtatt < 1:453554/91‑1 (MQ=255) gTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtattt > 1:2290391/1‑91 (MQ=255) gTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtattt > 2:1895174/1‑91 (MQ=255) tttATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtattta > 1:1490645/1‑91 (MQ=255) ttATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGtatttat > 2:1645960/1‑91 (MQ=255) ttATATCATTTTACGTTTGTAATATACTTTATTTCTACAAACACAAAAAACCGATGCATAAGCATTGGCTCATTTGTAATACAGtatttat > 2:2232423/1‑91 (MQ=255) atatCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTACTACAGTatttattt > 2:2767396/1‑91 (MQ=255) tCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTAtttatttacc > 2:2386475/1‑89 (MQ=255) cATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCt > 2:1705427/1‑91 (MQ=255) aTTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTa < 1:2410183/91‑1 (MQ=255) aTTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTa > 1:820832/1‑91 (MQ=255) ttttACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTaa < 1:3580114/91‑1 (MQ=255) ttttACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTaa < 1:1792622/91‑1 (MQ=255) ttACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATc < 1:796647/91‑1 (MQ=255) tACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATcc < 1:2486858/91‑1 (MQ=255) aCGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATccc > 1:3227151/1‑91 (MQ=255) cGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCa < 2:2138022/91‑1 (MQ=255) cGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCa < 1:1265048/91‑1 (MQ=255) gTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATGCCAt < 1:3127168/91‑1 (MQ=255) ttGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCActt > 2:464487/1‑91 (MQ=255) tGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCAtttt < 1:1541630/91‑1 (MQ=255) aCTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATc < 1:335290/91‑1 (MQ=255) cTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCt > 1:2200702/1‑91 (MQ=255) ataAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTCTTTATTTATCTAATCCCATTTTCTCTTg > 1:2093396/1‑91 (MQ=255) ataAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTCGTACTACAGGATTTAATTATCTAATCCCATTTTATCTTg > 2:520979/1‑91 (MQ=255) taAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGa > 2:2088252/1‑91 (MQ=255) attattTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAAcc < 2:711755/91‑1 (MQ=255) ttattTCTACAAACACTAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGAATTTATCTCACTATTCCCATTTTTTCTTGatcta > 2:582296/1‑87 (MQ=255) tttCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACAt < 1:2530850/91‑1 (MQ=255) ttCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTCATCCCATTTTATCTTGAACCACATc > 1:3703101/1‑91 (MQ=255) cTACAACCACAAAAAACCGATGCATACGCACCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCTTTTTATCTTGAACCACATCAg < 1:3347456/91‑1 (MQ=255) cTACAAACACAAAAAACTGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAg < 1:3381642/91‑1 (MQ=255) cTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAg < 2:3108872/91‑1 (MQ=255) cTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAAT‑CCATTGTATCTTGAACCACATCAGc < 2:3455041/91‑1 (MQ=255) tACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGc > 1:2605281/1‑91 (MQ=255) aCAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCt > 2:2101030/1‑91 (MQ=255) aCAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCt > 1:1479138/1‑91 (MQ=255) cAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTa > 2:1333379/1‑91 (MQ=255) cAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTa < 1:647088/91‑1 (MQ=255) cAAACACAAAAAACCGATGCATACGCATCGGCTCATCTGTAATACAGTATTTATTTATCCAATCCCACTTTATCTTGAACCACACCAGCTa > 2:1332882/1‑91 (MQ=255) aaaCACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTAt < 2:2074666/91‑1 (MQ=255) aaaCACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTAt < 2:1511983/91‑1 (MQ=255) aaCACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTAtt > 2:958727/1‑91 (MQ=255) acaAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATttgt > 1:2047272/1‑91 (MQ=255) acaAAAAACCGATGCATACGCATCGGCTCACTTGTAATACAGTCTTTCTCTCTCTACTCCCATTTTATCTTGAACCACCTCAGCTATttgt > 2:2943613/1‑91 (MQ=255) aaaaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTtgttgt < 2:2058134/91‑1 (MQ=255) agaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTCTTTATCTAATCCCATTTTATCTTGAGCCACATCAGCTATTTGTtgtg < 2:3759698/89‑1 (MQ=255) aaaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTtgtg > 1:676919/1‑91 (MQ=255) aaaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTtgtg < 1:35273/91‑1 (MQ=255) aaaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTtgtg < 1:1331460/91‑1 (MQ=255) aaaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGc > 2:3363690/1‑91 (MQ=255) aaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCa < 2:1048551/91‑1 (MQ=255) aaCCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCa < 2:2505925/91‑1 (MQ=255) aCCGATGCATACGCATCGGCTCATTCGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCaa < 1:3683199/91‑1 (MQ=255) cGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAAt > 2:3207712/1‑91 (MQ=255) cGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAAt > 2:2329929/1‑91 (MQ=255) tGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCtt > 2:2227765/1‑91 (MQ=255) tGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCtt < 1:571508/91‑1 (MQ=255) tGCATACGCATCGGCGCCTTTGTACTACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCtt > 2:322320/1‑91 (MQ=255) gCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCttt > 1:2908183/1‑91 (MQ=255) cATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTc > 1:2902111/1‑91 (MQ=255) cATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTc > 1:1734347/1‑91 (MQ=255) tACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAg < 2:3580595/91‑1 (MQ=255) tACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAg < 2:2333106/91‑1 (MQ=255) tACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAg < 2:885611/91‑1 (MQ=255) tACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAg < 1:2327266/91‑1 (MQ=255) tACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCGTTCAg < 2:517987/91‑1 (MQ=255) cGCATCGGCTCATTCGTAATACAGTCTTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTAAgaa > 2:748081/1‑89 (MQ=255) | TACATATTGATTACTTTGTTTTTATTTTGTTTATATCATTTTACGTTTGTACTATAAATTATTTCTACAAACACAAAAAACCGATGCATACGCATCGGCTCATTTGTAATACAGTATTTATTTATCTAATCCCATTTTATCTTGAACCACATCAGCTATTTGTTGTGCAAATCTTTCAGCA > USA300TCH1516_ALE/2286247‑2286427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |