| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,517,782 | T→A | 12.1% | L950L (CTA→CTT) | narG ← | Respiratory nitrate reductase 1 alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,517,782 | 0 | T | A | 12.1% | 552.2 / 56.4 | 183 | L950L (CTA→CTT) | narG | Respiratory nitrate reductase 1 alpha chain |
| Reads supporting (aligned to +/- strand): ref base T (71/89); new base A (7/15); total (78/105) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01 | |||||||||||
Only 100 of 190 total aligned reads displayed.
AGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAAC > USA300TCH1516_ALE/2517692‑2517872 | aaaTATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAg < 1:3396829/91‑1 (MQ=255) aaaTATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAg < 1:1847724/91‑1 (MQ=255) aaaTATCTTTTAACGACATTCCAGTTTGTTCTTCGAGATCTTCATATGCTTTTTGTGATACTTTACCATTCGTAGCAGATGAAATACTTAg > 2:1579412/1‑91 (MQ=255) aaTATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGCAATACTAAGt < 1:752097/91‑1 (MQ=255) aaTATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGt < 2:3394889/91‑1 (MQ=255) aaTATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGt > 2:726169/1‑91 (MQ=255) atatCTTTTAACGGCTTTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTCCCATTCGTAGCAGATGAAATACTTAGTa < 2:827964/91‑1 (MQ=255) tatCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTAt > 2:2064904/1‑91 (MQ=255) tatCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTAt < 2:1260840/91‑1 (MQ=255) atCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTAtt > 1:2328096/1‑91 (MQ=255) tCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTAttt < 1:2892364/91‑2 (MQ=38) tCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGCAATACTAAGTAttt < 2:3046473/91‑2 (MQ=255) cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTAtttc < 1:2342482/91‑3 (MQ=38) cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGc < 2:1006879/91‑1 (MQ=255) tttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCAt > 2:1758167/1‑91 (MQ=255) tttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCAt < 2:2714821/91‑1 (MQ=255) ttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATc < 1:3257492/91‑1 (MQ=38) ttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATc < 1:1165544/91‑1 (MQ=38) ttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATc < 1:907110/91‑1 (MQ=37) aaCGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTTTTTTATTTTTCCATTCGTAGCAGATGAAATACTTAGTATTGCATCAg > 2:2369488/1‑91 (MQ=255) aCGGCATTCCAGTTTGTTCTTCGAGATCTTCGTATGATTTTTGTGATAATTTAGCATGCGTAGCAGATGAAATACTTAGTATTGCATCAGc < 1:3157735/91‑1 (MQ=255) cGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATCTGCt < 2:2603314/91‑1 (MQ=25) cGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCt < 2:2418777/91‑1 (MQ=255) ggCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTa < 1:1902760/91‑1 (MQ=255) ggCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTa < 2:2677416/91‑1 (MQ=255) gCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTAc < 1:1783855/91‑1 (MQ=255) cATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACa > 1:2157444/1‑91 (MQ=255) cATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACa > 1:1672946/1‑91 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 2:470859/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 1:104777/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 1:3582912/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt > 2:3487059/1‑91 (MQ=255) ccAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTAc < 2:2542254/91‑1 (MQ=255) cAGTTGGTTCTTCGAGATCTTCATATGATTGTTGTGATGATTTAGCATTGGTAGCAGATGAGATACTTAGTATTGCATCAGCTACATTACg < 2:591350/91‑1 (MQ=255) aGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGt > 1:443100/1‑91 (MQ=255) tttGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGc > 2:2317227/1‑91 (MQ=255) tGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTg > 1:1272400/1‑91 (MQ=255) tGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTg > 1:1745314/1‑91 (MQ=255) ttcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTa < 1:1191144/91‑1 (MQ=255) tcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTAt < 1:3455753/91‑1 (MQ=255) tcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTAt < 2:3221637/91‑1 (MQ=255) cttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATc < 1:3845333/91‑1 (MQ=255) tcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCaa < 1:1364718/91‑1 (MQ=255) gagaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATa < 1:3663749/91‑1 (MQ=255) aTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGt < 1:1651330/91‑1 (MQ=255) aTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAACTACATTACGTGCTGTATCAATACGt < 2:2200769/91‑1 (MQ=255) tCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTg > 1:2163110/1‑91 (MQ=255) cTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTgg > 2:143906/1‑91 (MQ=255) cTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTgg < 2:644491/91‑1 (MQ=255) cTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTgg > 1:3542505/1‑91 (MQ=255) cATATGATTTTTGTGATAATTTACCATTCGTATCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACg < 1:1234099/91‑1 (MQ=255) cATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACg < 2:3231986/91‑1 (MQ=255) tatGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCGGCTACATTACGTGCTGTATCAATACGTGGACGAt < 2:3619143/91‑1 (MQ=255) tatGATTATTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAt < 1:1953284/91‑1 (MQ=255) atGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAtt > 2:2088317/1‑91 (MQ=255) gATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCg < 2:3641994/91‑1 (MQ=255) ttttGTGATAATTTACCATTCGTAGCAGATGAAATACTGAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctc < 2:2957818/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTTCATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:3340429/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctct > 2:3537280/1‑91 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:2953973/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:666547/91‑1 (MQ=255) tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTca < 2:2926893/91‑1 (MQ=255) tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTca < 1:1235559/91‑1 (MQ=255) gtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTcac > 1:634149/1‑91 (MQ=255) gtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTcac < 1:3535159/91‑1 (MQ=255) gATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAg < 2:3894786/91‑1 (MQ=255) gATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAg > 1:2617120/1‑91 (MQ=255) ttAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCTCAGa > 2:3127895/2‑91 (MQ=255) aTAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGa < 1:2780704/91‑1 (MQ=255) aTAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGa > 2:3127790/1‑91 (MQ=255) tAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGaa < 1:1037870/91‑1 (MQ=255) aaTTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAat < 2:3844989/91‑1 (MQ=255) tttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatca < 1:2003992/91‑1 (MQ=255) tttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatca > 1:2052680/1‑91 (MQ=255) ttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat > 2:1289828/1‑91 (MQ=255) ttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat > 1:218425/1‑91 (MQ=255) cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGTATCattatt > 2:1640115/1‑91 (MQ=255) cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGCATCATCAtt > 2:2481983/1‑91 (MQ=255) cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCAtt < 2:1147632/91‑1 (MQ=255) ttCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTg > 2:1946329/1‑91 (MQ=255) tCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGt > 1:243572/1‑91 (MQ=255) tCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGt > 1:242829/1‑91 (MQ=255) gTAGCTGATGCAATACTAAGTATTTCATCTGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTAt > 1:1322373/1‑91 (MQ=25) gCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTTCTGTATCAATACGTTGGCGATTCGCTCTCACAGAATCATCATTTGTATccc > 2:3280950/1‑89 (MQ=255) aGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCAGTc > 2:1202678/1‑91 (MQ=255) aGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTc < 2:901614/91‑1 (MQ=255) gATGCAATACTAAGTATTTCATCTGCCACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTcc > 2:2238965/1‑91 (MQ=38) aTGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCa < 1:697701/91‑1 (MQ=255) gcaaTACTAAGTATTTCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACg > 1:3754757/3‑91 (MQ=255) gcaaTACTAAGTATTTCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACg > 1:2534709/3‑91 (MQ=255) acgtAGTATTGCATCAGCTACATTACGGGCTGTAGCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt < 2:3762962/88‑1 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt < 2:2577761/91‑1 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt < 2:3763016/91‑1 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt > 1:299123/1‑91 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt > 1:298663/1‑91 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt > 1:2041804/1‑91 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCACACAGAATCATCATTTGTATCACACCACGTACCt < 2:1107046/91‑1 (MQ=255) cTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTa < 1:139368/91‑1 (MQ=255) ttAGTATTGGATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTaa < 1:3787223/91‑1 (MQ=255) tAGCATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAAc < 1:3062861/91‑1 (MQ=255) | AGAAATATCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAAC > USA300TCH1516_ALE/2517692‑2517872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |