| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,517,789 | G→T | 8.1% | A948E (GCA→GAA) | narG ← | Respiratory nitrate reductase 1 alpha chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,517,789 | 0 | G | T | 8.1% | 618.1 / 27.2 | 187 | A948E (GCA→GAA) | narG | Respiratory nitrate reductase 1 alpha chain |
| Reads supporting (aligned to +/- strand): ref base G (71/99); new base T (8/8); total (80/107) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 194 total aligned reads displayed.
TCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTT > USA300TCH1516_ALE/2517699‑2517879 | cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGc < 2:1006879/91‑1 (MQ=255) cTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGAAAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGc < 2:846491/91‑1 (MQ=255) tttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCAt > 2:1758167/1‑91 (MQ=255) ttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATc < 1:1165544/91‑1 (MQ=38) ttAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATc < 1:3210991/91‑1 (MQ=38) tAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATct < 1:2331376/91‑2 (MQ=37) aCGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCTGATGCAATACTAAGTATTTCATctgc < 2:3142803/91‑4 (MQ=37) cGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCt < 2:2418777/91‑1 (MQ=255) ggCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTa < 1:1902760/91‑1 (MQ=255) gCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTAc < 1:1783855/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt > 2:3487059/1‑91 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 2:470859/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 1:3582862/91‑1 (MQ=255) ttCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAtt < 1:104777/91‑1 (MQ=255) ccAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTAc > 1:2046530/1‑91 (MQ=255) cAGTTGGTTCTTCGAGATCTTCATATGATTGTTGTGATGATTTAGCATTGGTAGCAGATGAGATACTTAGTATTGCATCAGCTACATTACg < 2:591350/91‑1 (MQ=255) aGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGt > 1:443100/1‑91 (MQ=255) tttGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGc < 1:2010395/91‑1 (MQ=255) tttGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGc < 1:2446355/91‑1 (MQ=255) tGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTg > 1:1272400/1‑91 (MQ=255) tGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTg > 1:1745315/1‑91 (MQ=255) ttcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTa < 1:1191144/91‑1 (MQ=255) tcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTAt < 2:3221637/91‑1 (MQ=255) tcttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTAt < 1:3455753/91‑1 (MQ=255) cttcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATc < 1:3845333/91‑1 (MQ=255) tcGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCaa < 1:1364742/91‑1 (MQ=255) gagaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATa < 1:2622849/91‑1 (MQ=255) agaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATAc < 2:2681522/91‑1 (MQ=255) gaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACg < 1:3089714/91‑1 (MQ=255) gaTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTACATCAGCTACATTACGTGCTGTATCAATACg > 1:1743721/1‑91 (MQ=255) aTCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGt > 2:3243496/1‑91 (MQ=255) tCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTg > 1:2163110/1‑91 (MQ=255) cTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTgg > 2:143906/1‑91 (MQ=255) tCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGAc < 1:896164/91‑1 (MQ=255) cATATGATTTTTGTGATAATTTACCATTCGTATCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACg < 1:1234099/91‑1 (MQ=255) atatGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGa < 2:2550137/91‑1 (MQ=255) tatGATTATTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAt < 1:1953284/91‑1 (MQ=255) atGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAtt > 2:2088317/1‑91 (MQ=255) atGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGAtt < 2:2230261/91‑1 (MQ=255) gATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCg > 1:460081/1‑91 (MQ=255) tttttGTGATAATTTACCAGTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGcc > 2:3451747/1‑90 (MQ=255) ttttGTGATAATTTACCATTCGTAGCAGATGAAATACTGAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctc < 2:2957818/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTTCATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:3340319/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:2953973/91‑1 (MQ=255) tttGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGctct < 1:2071518/91‑1 (MQ=255) tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTca < 2:2926893/91‑1 (MQ=255) tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTca < 1:1235559/91‑1 (MQ=255) tgtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTca > 1:3638732/1‑91 (MQ=255) gtgATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTcac < 1:3535159/91‑1 (MQ=255) gtaaTAATTCACCATTCGTAGCAGATGAAATACTTAGTATTTCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGTTCTcac > 2:2623957/4‑91 (MQ=255) aTAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGa > 2:3127790/1‑91 (MQ=255) tAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGaa < 1:1037870/91‑1 (MQ=255) tAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGaa > 2:357573/1‑91 (MQ=255) aaTTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAat < 2:3845930/91‑1 (MQ=255) ttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat > 2:1289828/1‑91 (MQ=255) ttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat > 1:218425/1‑91 (MQ=255) ttACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAatcat < 2:2594743/91‑1 (MQ=255) ttACCATGCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACAGTACGTGCTGGATCAATACGTGGGCGATTCGCTCGCACAGAatcat < 2:688112/91‑1 (MQ=255) aCCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAAtcatca < 2:2506451/91‑1 (MQ=255) cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGTATCattatt > 2:1640115/1‑91 (MQ=255) cATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCAtt > 1:658521/1‑91 (MQ=255) ttCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTg < 2:201698/91‑1 (MQ=255) tCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGt > 1:242929/1‑91 (MQ=255) tCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGt > 1:189993/1‑91 (MQ=255) cGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTa > 1:68692/1‑91 (MQ=255) gTAGCTGATGCAATACTAAGTATTTCATCTGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTAt > 1:1322373/1‑91 (MQ=25) gTAGCAGATGAAATACTTAGTATTGCCTCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTAt > 2:2242061/1‑91 (MQ=255) tAGCTGATGCAATACTAAGTATTTCATCTGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATc > 2:362590/1‑91 (MQ=25) agctgATGCAATACTAAGTATTTCATCTGTTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCa > 2:1213026/5‑91 (MQ=25) gCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTTCTGTATCAATACGTTGGCGATTCGCTCTCACAGAATCATCATTTGTATccc > 2:3280950/1‑89 (MQ=255) cAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACt > 2:624995/1‑91 (MQ=255) aGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTc < 2:901614/91‑1 (MQ=255) aGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTc > 2:1202659/1‑91 (MQ=255) gcaaTACTAAGTATTTCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACg > 1:3754757/3‑91 (MQ=255) aTACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTAc > 1:2985567/1‑91 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGCACCATCATTTGTATCACTCCACGTAccc > 2:2318946/1‑90 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt > 1:299057/1‑91 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCt < 2:3763016/91‑1 (MQ=255) aCTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCACACAGAATCATCATTTGTATCACACCACGTACCt < 2:1107046/91‑1 (MQ=255) ttAGTATTGGATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTaa < 1:3787223/91‑1 (MQ=255) ttAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTaa > 1:2541822/1‑91 (MQ=255) tAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAAc > 2:2138097/1‑91 (MQ=255) tAGCATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAAc < 1:3062860/91‑1 (MQ=255) aGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTCCCTcaca > 2:341414/1‑91 (MQ=255) aGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACa > 1:3268603/1‑91 (MQ=255) aGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACa < 1:1671496/91‑1 (MQ=255) aGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACa > 2:3816499/1‑91 (MQ=255) aGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACa < 1:2912822/91‑1 (MQ=255) gTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt > 1:3399240/1‑91 (MQ=255) gTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt < 2:1828195/91‑1 (MQ=255) gTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACAt < 1:2812951/91‑1 (MQ=255) tATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATa < 1:239184/91‑1 (MQ=255) ttGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACt < 1:1610488/91‑1 (MQ=255) ttGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACt < 2:3655258/91‑1 (MQ=255) ttGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACt < 1:1353795/91‑1 (MQ=255) tGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACtt < 1:3199425/91‑1 (MQ=255) tGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACtt < 1:1456116/91‑1 (MQ=255) gCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACttt < 1:2169510/91‑1 (MQ=255) gCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACttt < 1:965302/91‑1 (MQ=255) gCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACttt > 2:2414753/1‑91 (MQ=255) | TCTTTTAACGGCATTCCAGTTTGTTCTTCGAGATCTTCATATGATTTTTGTGATAATTTACCATTCGTAGCAGATGAAATACTTAGTATTGCATCAGCTACATTACGTGCTGTATCAATACGTGGACGATTCGCTCTCACAGAATCATCATTTGTATCACTCCACGTACCTAACATACTTT > USA300TCH1516_ALE/2517699‑2517879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |