| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 581,901 | G→C | 6.4% | V13L (GTG→CTG) | nusG_2 → | Transcription termination/antitermination protein NusG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 581,901 | 0 | G | C | 6.4% | 699.7 / 23.8 | 203 | V13L (GTG→CTG) | nusG_2 | Transcription termination/antitermination protein NusG |
| Reads supporting (aligned to +/- strand): ref base G (93/97); new base C (10/3); total (103/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 211 total aligned reads displayed.
GATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTAGAG > USA300TCH1516_ALE/581811‑581991 | gATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAg < 2:3655703/91‑1 (MQ=255)gATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAg > 2:2771695/1‑91 (MQ=255) ttAGGAATTACAGCATTGAAAAATTTATTATTGCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCACTGc < 2:1935933/91‑1 (MQ=255) tAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGca > 2:229746/1‑91 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat > 1:1274761/1‑91 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat > 1:1276886/1‑91 (MQ=255) aGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat < 2:3342424/91‑1 (MQ=255) aGGAATTACAGCATCGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcat < 2:1253767/91‑1 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata > 2:1675309/1‑91 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata > 1:2586356/1‑91 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata > 1:2838615/1‑91 (MQ=255) ggAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTG‑AGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcatac < 1:1338951/91‑1 (MQ=255) ggAATTACAGCAGTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcata < 2:2003761/91‑1 (MQ=255) gAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcatac < 2:3529785/91‑1 (MQ=255) aaTTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcataca < 2:2222423/91‑1 (MQ=255) aTTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGcatacat < 1:3527243/91‑1 (MQ=255) aaaGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATAtt < 2:891430/89‑1 (MQ=255) aCAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATAtt > 1:842632/1‑91 (MQ=255) aCAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATAtt > 1:1058094/1‑91 (MQ=255) cAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTc > 1:3070702/1‑91 (MQ=255) aGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCt < 1:1592206/91‑1 (MQ=255) cATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTgg > 2:2588778/1‑91 (MQ=255) aTTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGa < 1:1123629/91‑1 (MQ=255) aTTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGAAAAGCGTTGGTATGCAGCGCATACATATTCTGGa > 2:3739310/1‑91 (MQ=255) ttGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGat > 1:1783822/1‑91 (MQ=255) gAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGatat < 1:1396677/91‑1 (MQ=255) gAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGatat < 1:1614042/91‑1 (MQ=255) gAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTATGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGatat < 1:2514603/91‑1 (MQ=255) aaaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATg > 2:2033198/1‑91 (MQ=255) aaaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATg < 2:2164103/91‑1 (MQ=255) aaaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATg > 2:3549205/1‑91 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAACGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 2:3906276/1‑91 (MQ=255) aaaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGa > 1:1420317/1‑91 (MQ=255) aaaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGaa < 1:321214/91‑1 (MQ=255) aaTTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGaaa > 2:660491/1‑91 (MQ=255) tttATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAAt < 2:857360/91‑1 (MQ=255) tattTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGt < 1:2725166/91‑1 (MQ=255) tCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaa > 2:3453700/1‑91 (MQ=255) gTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTaaaaa > 2:2889570/1‑91 (MQ=255) ttAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAg < 1:3645991/91‑1 (MQ=255) ttAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAg < 1:3647697/91‑1 (MQ=255) gaggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAt > 1:2885768/1‑91 (MQ=255) gaggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAt > 1:2319042/1‑91 (MQ=255) aggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAtt < 1:1710251/91‑1 (MQ=255) aggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAtt > 2:1410914/1‑91 (MQ=255) aggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTGGGTAGGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAtt < 2:2722036/91‑1 (MQ=255) ggagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAttt > 2:1431384/1‑91 (MQ=255) gagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGGAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTa < 1:3936698/91‑1 (MQ=255) gagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTa < 1:3223510/91‑1 (MQ=255) gagTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTa < 1:3936523/91‑1 (MQ=255) agTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAg < 2:678588/91‑1 (MQ=255) agTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAg > 1:247678/1‑91 (MQ=255) gTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGa > 1:2233606/1‑91 (MQ=255) gTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGa > 2:3690391/1‑91 (MQ=255) aGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGaaaaa > 1:3344200/1‑91 (MQ=255) aCATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAag < 1:2629839/91‑1 (MQ=255) cATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAaga > 2:913520/1‑91 (MQ=255) cATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAaga < 2:375799/91‑1 (MQ=255) cATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAaga < 1:480448/91‑1 (MQ=255) tGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGt < 1:1459111/91‑1 (MQ=255) gTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTa > 2:2853214/1‑91 (MQ=255) tCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAg < 1:968348/91‑1 (MQ=255) tCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAg < 2:3245054/91‑1 (MQ=255) cTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGa > 1:504334/1‑91 (MQ=255) cTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCGGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGa < 1:3061796/91‑1 (MQ=255) tGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTATGGATATGAAAATAAAGTTAAAAAAAAAAAAAAAAAAAGAGTAGaa < 2:1114108/91‑1 (MQ=255) gaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTa > 1:2349541/1‑91 (MQ=255) gaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTa < 1:18022/91‑1 (MQ=255) gaagTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTa < 1:476357/91‑1 (MQ=255) gaagTTGGCGCAAAGCGTTGGTATGCAGTGCAGACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTa < 2:2398956/91‑1 (MQ=255) agTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATg < 1:1192863/91‑1 (MQ=255) agTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATg < 1:3453276/91‑1 (MQ=255) agTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAAAAAAAAAAAAAAAGAGTAGAATCTATg < 2:525350/91‑1 (MQ=255) tGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGCAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAat < 1:3140233/91‑1 (MQ=255) tGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAat > 1:2174640/1‑91 (MQ=255) gcgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAatat > 2:2405440/1‑91 (MQ=255) gcgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAatat > 1:67807/1‑91 (MQ=255) cgcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATg < 1:1988687/91‑1 (MQ=255) gcAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGa > 2:3609186/1‑91 (MQ=255) cAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGAc < 1:3822058/91‑1 (MQ=255) cAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGAc < 1:310683/91‑1 (MQ=255) aaGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGCTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTg > 1:3141928/1‑91 (MQ=255) aGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGa > 1:2763651/1‑91 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc > 2:2809120/1‑91 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAAc > 2:44240/1‑91 (MQ=255) cGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAAAAAAAAAAAAAGAGTAGAATCTATGAATATGACTGAAc < 2:1590771/91‑1 (MQ=255) gTTGGAATGCAGAGAATAAATATTCTGGATATGAAAAAAAAGTAAAAAAAAAATAAGAAAAAAGAGTAGAATCTATGAATATGACTGAACa < 2:852770/91‑1 (MQ=255) tGGTATGCAGTGCATACATATTCTGGATATGCAAATAAAGTTAAACAGAATTCAGAAAAAAGAGTAGAATCTATGAATATTACTGAACaaa > 2:760553/1‑91 (MQ=255) atgTATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACaaa > 1:3625650/3‑91 (MQ=255) ggTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAAt > 1:2966092/1‑91 (MQ=255) gTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATc > 2:1614253/1‑91 (MQ=255) tATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCt > 1:3751433/1‑91 (MQ=255) tATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAATGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCt > 2:3059794/1‑91 (MQ=255) tATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCt > 2:3147181/1‑91 (MQ=255) tATGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCt > 2:1648934/1‑91 (MQ=255) aTGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCtt < 1:63492/91‑1 (MQ=255) tGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCttt > 2:2542229/1‑91 (MQ=255) tGCACTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCttt > 2:1592921/1‑91 (MQ=255) gCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTa > 1:3289629/1‑91 (MQ=255) aGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTaga > 2:3087004/1‑91 (MQ=255) | GATTTAGGAATTACAGCATTGAAAAATTTATTATTTCGTTAGAGGAGTGAAGACATGTCTGAAGAAGTTGGCGCAAAGCGTTGGTATGCAGTGCATACATATTCTGGATATGAAAATAAAGTTAAAAAGAATTTAGAAAAAAGAGTAGAATCTATGAATATGACTGAACAAATCTTTAGAG > USA300TCH1516_ALE/581811‑581991 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |