BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	639,592	C→G	5.3%	A33G (GCC→GGC)	USA300HOU_RS03045 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	639,592	0	C	G	5.3%	672.8 / 20.4	189	A33G (GCC→GGC)	USA300HOU_RS03045	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (99/80); new base G (4/6); total (103/86)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 194 total aligned reads displayed.

ATAGAGAAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCATAA  >  USA300TCH1516_ALE/639502‑639682

 tAGAGAAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATCTGTATGcc                                                                                           >  2:2476141/1‑91 (MQ=255)

   gagaAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcat                                                                                         <  1:3527007/91‑1 (MQ=255)

   gagaAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcat                                                                                         <  1:2684900/91‑1 (MQ=255)

    agaAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCCTGACTTCGAAAAACATATGTATGCcgta                                                                                        >  1:1645868/1‑91 (MQ=255)

     gaAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatac                                                                                       <  1:3493884/91‑1 (MQ=255)

     gaAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatac                                                                                       >  1:1294410/1‑91 (MQ=255)

      aaaCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGGcataca                                                                                      <  2:3945601/91‑1 (MQ=255)

      aaaCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcataca                                                                                      <  1:2150456/91‑1 (MQ=255)

       aaCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatacat                                                                                     >  2:662319/1‑91 (MQ=255)

       aaCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatacat                                                                                     >  2:1768964/1‑91 (MQ=255)

       aaCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatacat                                                                                     >  1:3384460/1‑91 (MQ=255)

        aCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatacata                                                                                    >  1:3781582/1‑91 (MQ=255)

        aCTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCcatacata                                                                                    >  1:89295/1‑91 (MQ=255)

         cTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATCATTTTGAAAAACATATGTATGCCATACatat                                                                                   >  1:3165313/1‑91 (MQ=255)

          tttAATAGCGCGAATTGAGCAAGAATGAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACatata                                                                                  <  2:191063/91‑1 (MQ=255)

          tttAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGGCATACatatg                                                                                  <  1:3673530/91‑2 (MQ=255)

          tttAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGGCATACatatg                                                                                  <  1:714176/91‑2 (MQ=255)

          tttAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACatata                                                                                  <  2:494459/91‑1 (MQ=255)

           ttAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACAATAAAAAAAAAAAACATATGTATGCCATACatatat                                                                                 <  2:2890036/91‑1 (MQ=255)

            tAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATAtt                                                                                <  1:1758185/91‑1 (MQ=255)

            tAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATAtt                                                                                >  1:145097/1‑91 (MQ=255)

             aaTAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGAGTTTGAAAAACATATGTATGCCATACATATATTa                                                                               >  1:3243713/1‑91 (MQ=255)

              aTAGCACGGATTGAGCAGGGATTAGTACAAGCGGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTaa                                                                              <  1:3387310/91‑1 (MQ=255)

              aTAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTaa                                                                              >  2:468008/1‑91 (MQ=255)

              aTAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTaa                                                                              >  2:2259622/1‑91 (MQ=255)

               tAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAAc                                                                             <  1:1072123/91‑1 (MQ=255)

               tAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAAc                                                                             <  1:2379618/91‑1 (MQ=255)

                  cACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATc                                                                          <  2:1090700/91‑1 (MQ=255)

                    cGAATTGAGCAAGAATTAGTACAAGCAGCGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCtt                                                                        <  1:18136/91‑1 (MQ=255)

                    cGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCtt                                                                        >  2:2413984/1‑91 (MQ=255)

                    cGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCtt                                                                        <  1:20205/91‑1 (MQ=255)

                     gAATTGAGCAAGAAATAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCttt                                                                       >  1:972479/1‑91 (MQ=255)

                       aTTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTtat                                                                     <  1:2172437/91‑1 (MQ=255)

                       aTTGAGCAAGAATTAGAACAAGCAGAGCAGGCACAGCATGAAAAAAAAAAAAAAAAACATATGTATGCCATACATATATTAACATCTTtat                                                                     <  2:132807/91‑1 (MQ=255)

                         tGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTatat                                                                   <  2:1033514/91‑1 (MQ=255)

                           aGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGc                                                                 <  1:1926146/91‑1 (MQ=255)

                           aGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGc                                                                 >  1:1641197/1‑91 (MQ=255)

                           aGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGc                                                                 >  1:1079755/1‑91 (MQ=255)

                            gCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCt                                                                <  2:3255721/91‑1 (MQ=255)

                            gCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCt                                                                <  2:416890/91‑1 (MQ=255)

                            gCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCt                                                                >  1:65245/1‑91 (MQ=255)

                               aGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCa                                                             >  2:827029/1‑91 (MQ=255)

                                gAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCaa                                                            >  1:342979/1‑91 (MQ=255)

                                    aaGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATc                                                        <  1:1704088/90‑1 (MQ=255)

                                     aGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCa                                                       <  2:2611234/91‑1 (MQ=255)

                                      gTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCaa                                                      >  1:1082543/1‑91 (MQ=255)

                                        aCAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAAt                                                    >  1:2118105/1‑91 (MQ=255)

                                         cAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATa                                                   >  1:3100682/1‑91 (MQ=255)

                                          aaGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATac                                                  <  1:2824411/91‑1 (MQ=255)

                                             cagagcagGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATAcacc                                               <  2:859494/91‑1 (MQ=255)

                                               gagcagGCACAGCATGACCATAACATTAAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATAcaccac                                             <  2:1649363/91‑1 (MQ=255)

                                                 gcagGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACat                                           <  1:1237072/91‑1 (MQ=255)

                                                 gcagGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACat                                           >  1:1780725/1‑91 (MQ=255)

                                                 gcagGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACat                                           <  1:2117323/91‑1 (MQ=255)

                                                   agGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACatat                                         >  1:3506693/1‑91 (MQ=255)

                                                   agGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACatat                                         >  1:3505872/1‑91 (MQ=255)

                                                   agGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACatat                                         <  2:3753892/91‑1 (MQ=255)

                                                     gCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTg                                       >  1:1099266/1‑91 (MQ=255)

                                                     gCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTg                                       >  1:906317/1‑91 (MQ=255)

                                                         aGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGa                                   <  1:1043817/91‑1 (MQ=255)

                                                         aGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGa                                   <  2:739231/91‑1 (MQ=255)

                                                         aGCATGACCATGACTTTGAAAAACATATGTATGCCATACAGATATTAACATCTTTATATGCTTTAACATCAAATACACCACATATAGGTGa                                   >  2:3253858/1‑91 (MQ=255)

                                                           catgaccatgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGaac                                 >  2:3169498/1‑91 (MQ=255)

                                                             tgaccatgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTATATATGCTTCAACATCAAATACACCACATATTGGTGaacaa                               >  2:3477944/1‑91 (MQ=255)

                                                              gaccatgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGaacaac                              <  2:3840328/91‑1 (MQ=255)

                                                                ccatgacTTTGAAAAACATATGTATGCCATACATATATTAACATGTTTATATGGTTCAACATCAAATACACCACATATTGGTGAAcaacaa                            <  1:3153667/91‑1 (MQ=255)

                                                                ccatgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAAcaacaa                            >  1:340659/1‑91 (MQ=255)

                                                                 catgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACaaa                           <  1:1310486/91‑1 (MQ=255)

                                                                 catgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACaaa                           <  1:1310803/91‑1 (MQ=255)

                                                                 catgacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACaaa                           <  1:450957/91‑1 (MQ=255)

                                                                    gacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGa                        >  1:157407/1‑91 (MQ=255)

                                                                    gacTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGa                        >  1:3006458/1‑91 (MQ=255)

                                                                      cTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGATTCAACATCAAATACACCACATATTGGTGAACAACAAATGAAt                      >  2:2362702/1‑91 (MQ=255)

                                                                       tttGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAAtc                     >  1:2910108/1‑91 (MQ=255)

                                                                        ttGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAAtcg                    <  1:1695337/91‑1 (MQ=255)

                                                                        ttGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAAtcg                    >  2:3391949/1‑91 (MQ=255)

                                                                            aaaaCATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATcgtcgt                <  1:2329334/91‑1 (MQ=255)

                                                                              aaCATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTAt              >  1:2809242/1‑91 (MQ=255)

                                                                                cATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCCAATACACCACATATTGGTGAACAACAAATCAATCGTCGTAtta            >  1:3959001/1‑90 (MQ=255)

                                                                                cATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTAtta            >  2:3491300/1‑90 (MQ=255)

                                                                                cATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTg            >  2:3867232/1‑91 (MQ=255)

                                                                                cATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTg            >  1:223651/1‑91 (MQ=255)

                                                                                cATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTg            >  2:1514244/1‑91 (MQ=255)

                                                                                 atatGTATGGCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGc           >  2:1994949/1‑91 (MQ=255)

                                                                                 atatGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGc           <  2:2147617/91‑1 (MQ=255)

                                                                                   atgtatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTa         <  1:3295209/91‑1 (MQ=255)

                                                                                   atgtatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTa         >  2:1294916/1‑91 (MQ=255)

                                                                                    tgtatgGCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTaa        >  2:2992491/1‑91 (MQ=255)

                                                                                    tgtatgCCATACATATATTAACATCTTTATATGCTTCTACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTaa        >  1:3326308/1‑91 (MQ=255)

                                                                                     gtatgGCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAc       >  1:3728127/1‑91 (MQ=255)

                                                                                     gtatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAc       >  2:2343525/1‑91 (MQ=255)

                                                                                     gtatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAc       <  2:1645613/91‑1 (MQ=255)

                                                                                     gtatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAc       >  2:1358715/1‑91 (MQ=255)

                                                                                     gtatgCCATACATATATTAACATCTTGATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGGCGTATTGCTAAc       <  2:1645868/91‑1 (MQ=255)

                                                                                      tatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAcc      >  1:2090472/1‑91 (MQ=255)

                                                                                      tatgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAAcc      >  1:1183738/1‑91 (MQ=255)

                                                                                       atgCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCa     <  2:2660992/91‑1 (MQ=255)

                                                                                         gCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCATa   <  1:775702/91‑1 (MQ=255)

                                                                                         gCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCATa   <  1:2515015/91‑1 (MQ=255)

                                                                                          ccATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCATaa  >  2:323268/1‑91 (MQ=255)

ATAGAGAAACTTTAATAGCACGAATTGAGCAAGAATTAGTACAAGCAGAGCAGGCACAGCATGACCATGACTTTGAAAAACATATGTATGCCATACATATATTAACATCTTTATATGCTTCAACATCAAATACACCACATATTGGTGAACAACAAATGAATCGTCGTATTGCTAACCATAA  >  USA300TCH1516_ALE/639502‑639682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`