| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 740,322 | G→C | 21.4% | intergenic (+549/+39) | pitA_1 → / ← sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 740,322 | 0 | G | C | 21.4% | 657.5 / 188.0 | 287 | intergenic (+549/+39) | pitA_1/sle1_2 | Low‑affinity inorganic phosphate transporter 1/N‑acetylmuramoyl‑L‑alanine amidase sle1 |
| Reads supporting (aligned to +/- strand): ref base G (90/135); new base C (23/38); total (114/173) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
Only 100 of 318 total aligned reads displayed.
ATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAG > USA300TCH1516_ALE/740232‑740412 | tataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatga > 2:2669013/1‑91 (MQ=255) tataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatga < 2:2696779/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:866273/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgat < 2:1358663/91‑1 (MQ=255) ataaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACatcatg < 1:550338/91‑1 (MQ=255) taaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACatgatg > 1:273017/1‑91 (MQ=255) aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGAtgt < 2:1487012/91‑1 (MQ=255) aaatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCAtgtg < 2:3410215/91‑1 (MQ=255) aaatatGTATGATAGGGAAGGGAGAGATAATTTTTTGTACCAAAATGAAAATTAATTAAAAAAATAAAAAACTAGCAAT‑TCACATCAtgtg < 2:3196504/91‑1 (MQ=255) aatatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGa < 1:1878913/91‑1 (MQ=255) aatagGTATGATAGGGAAGGGGGAGATAATTTTTTGTACCAAAGTGAAAATTTTTGTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGa < 2:2790676/91‑1 (MQ=255) atatGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa > 2:3899502/1‑91 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a > 1:1816767/1‑91 (MQ=255) tatgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGa‑a > 2:3115832/1‑91 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 2:1558468/91‑1 (MQ=255) atgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagat < 1:3350024/91‑4 (MQ=39) atgtatgATAGGGAAGGGTGAGATAATTTTGTGTACCAAAATGAAAATTATTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑At < 2:3575473/91‑1 (MQ=255) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCTTAATTAAAAAACTAGCAATCTCACATGATGTGA‑Att > 2:3528040/1‑91 (MQ=255) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑Att < 1:1928475/91‑1 (MQ=255) tgtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagatt < 1:1606894/91‑5 (MQ=38) tgtaggATAGGGAAGGGTTAGATAATTTTCCGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGagatt < 2:963204/91‑5 (MQ=37) tggatgATAGTGAAGGGTAAGATAAGTGTAAGTACCAAAATAAAAAGTTATTAAAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑Att < 2:1264715/88‑1 (MQ=255) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg > 2:3813783/1‑91 (MQ=255) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg < 2:856651/91‑1 (MQ=255) gtatgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTg > 1:3841594/1‑91 (MQ=255) atgATAGGGAATGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTGCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCt < 2:3295160/91‑1 (MQ=37) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 1:586963/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:1597886/91‑1 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt > 1:157061/1‑91 (MQ=255) atgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAAGTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCt < 2:2350454/91‑1 (MQ=37) atgATAGAGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:1824145/91‑1 (MQ=255) ataaTAGGGAAGGGTGAGAAAATTTTTTGAAACAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCt < 2:2368705/88‑1 (MQ=255) tgATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTa < 2:906650/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAg < 1:1470865/91‑1 (MQ=255) gATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAg < 2:651079/91‑1 (MQ=37) aGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtat < 1:880013/91‑1 (MQ=255) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTTAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtata > 2:1905149/1‑91 (MQ=255) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 2:2936912/91‑4 (MQ=25) gggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTGTTCAAAAATAAAAAACTAGCAAT‑TCACATCATGTGAGATTGCTAGtttt < 2:3463600/91‑4 (MQ=25) ggAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCGCACATGATGTGA‑ATTGCTAGtatat > 1:3666721/1‑91 (MQ=255) ggAAGAGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGtatat < 2:3592254/91‑1 (MQ=255) aGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATc > 1:1061270/1‑91 (MQ=255) ggTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAg < 2:700640/91‑1 (MQ=255) gTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGt > 1:2088234/1‑91 (MQ=255) tGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTa < 1:1279931/91‑1 (MQ=255) gaTAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACaa > 1:202166/1‑91 (MQ=255) tAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatt < 1:3626535/91‑1 (MQ=255) tAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatt < 1:1185867/91‑1 (MQ=255) tAATTATTTGTACCAAAATGAAAAATTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatt < 2:549829/91‑1 (MQ=255) tttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttatt < 1:2537017/91‑1 (MQ=255) ttttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAatttattt < 2:1057549/91‑1 (MQ=255) ttatGTACCAAAATGAAAA‑TTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta < 2:3046873/88‑1 (MQ=255) tttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAAtttattta < 1:213969/91‑1 (MQ=255) ttGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATttaa > 2:2133492/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 2:461308/1‑91 (MQ=255) ccAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTtaattaat > 1:1710850/1‑91 (MQ=255) aaaaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATgg > 1:3754306/1‑91 (MQ=255) aaaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGa < 1:1624736/91‑1 (MQ=255) aaTGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGAt < 1:3135558/91‑1 (MQ=255) tGAAAATTTTTTTCAAAATTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGa > 2:2838238/1‑91 (MQ=255) gAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGaa < 1:448293/91‑1 (MQ=255) aaaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATg < 1:934749/91‑1 (MQ=255) aaTTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGc > 2:251353/1‑91 (MQ=255) tttttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCAt < 2:778898/91‑1 (MQ=255) tttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAg > 2:1736667/1‑91 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 1:508201/91‑1 (MQ=255) ttttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGc < 2:3176614/91‑1 (MQ=255) tttCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCt < 2:1648151/91‑1 (MQ=255) tCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAg < 2:3489920/91‑1 (MQ=255) cAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGa < 2:2394916/91‑1 (MQ=255) cAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGa > 1:3819265/1‑91 (MQ=255) aaaaaaTAAAAGACTAGCAATCTTACATGATGTGA‑ATTGCTAGTATATATCAGTACAATGTATTTAATTAATGGATGAATGCATAGCTAGa < 2:3017823/90‑1 (MQ=255) aaaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaa > 2:1209295/1‑91 (MQ=255) aaaaTAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGaaa > 1:2211353/1‑91 (MQ=255) aaaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCt < 1:240639/91‑1 (MQ=255) aaaaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATCGATGAATGCATAGCTAGAAACTTCTg < 2:2249643/91‑1 (MQ=255) aaaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGaa < 1:2585325/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg < 1:3533813/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg < 1:2897509/91‑1 (MQ=255) aaCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAg < 1:755246/91‑1 (MQ=255) aCTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGc < 1:285652/91‑1 (MQ=255) cTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCt < 2:2041835/91‑1 (MQ=255) cTAGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCt > 1:1429728/1‑91 (MQ=37) tAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTg < 2:1983948/91‑1 (MQ=255) aGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg > 1:1150749/1‑91 (MQ=255) aGCAAT‑TCACATCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTgg > 2:573699/1‑91 (MQ=38) aTCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAAtt > 2:1780123/1‑91 (MQ=255) ctcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGt < 1:3393298/91‑1 (MQ=255) tcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa < 1:400846/91‑1 (MQ=255) tcACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa > 2:709371/1‑91 (MQ=255) cacaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa > 2:3390518/1‑91 (MQ=255) cacaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa > 1:65698/1‑91 (MQ=255) cacaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTa > 1:698020/1‑91 (MQ=255) acaTGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGACTTgaaaa > 2:1188588/1‑87 (MQ=255) acaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTAc > 2:1945857/1‑91 (MQ=255) acaTCATGTGAGATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTAc > 1:3428182/1‑91 (MQ=255) caTGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACgg < 2:2205467/91‑1 (MQ=255) atgatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGt < 2:3723176/91‑1 (MQ=255) gatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAg < 1:81691/91‑1 (MQ=255) gatgTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAg < 1:1012009/91‑1 (MQ=255) | ATATAAATATGTATGATAGGGAAGGGTGAGATAATTTTTTGTACCAAAATGAAAATTTTTTTCAAAAATAAAAAACTAGCAATCTCACATGATGTGA‑ATTGCTAGTATATATCAGTACAATTTATTTAATTAATGGATGAATGCATAGCTAGAAACTTCTGAAGCTGGAATTGTACGGTAG > USA300TCH1516_ALE/740232‑740412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |