Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,232,465 G→C 100% A124P (GCG→CCG)  prkC → Serine/threonine‑protein kinase PrkC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,232,4650GC100.0% 94.8 / NA 31A124P (GCG→CCG) prkCSerine/threonine‑protein kinase PrkC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/13);  total (18/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAT  >  USA300TCH1516_ALE/1232328‑1232599
                                                                                                                                         |                                                                                                                                      
aagaagATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGTATGGCATTAAACATccga                                                                                                                                     >  1:237985/1‑140 (MQ=255)
  gaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAGTCAAATATTGGATGGCATTAAACATCCGCAt                                                                                                                                   >  1:119668/1‑141 (MQ=255)
      aTGACTGTTACTACTTAATAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATGAAGTGTTGACACAGCGATTAATTTTACGAATCAAATAGGGGATGGCATTAAACATCCGCATGata                                                                                                                               <  2:89014/141‑1 (MQ=255)
         aCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                            <  2:125096/141‑1 (MQ=255)
         aCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                            >  1:30774/1‑141 (MQ=255)
           tGTTACTACTTAGTAATGGAATATATTGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGt                                                                                                                          >  2:226669/1‑141 (MQ=255)
           tGTTACTACTTAGTAATGGAATATAACGAAGGTCCGACTTTGTCTGAGCATATTAAAAGTCACGGGCAATTAAGTGTTGACACAGCGATTAATTTAACGAATCAAATATTGGATGGCATTAAAAATCCGCATGAAATGCGt                                                                                                                          >  2:1561/1‑141 (MQ=255)
                ctattTAGTAATGAAATATATCGAAGGTCAGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAAGCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGt                                                                                                                     <  1:342198/141‑1 (MQ=255)
                    gtAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACAt                                                                                                                 >  2:201781/2‑141 (MQ=255)
                        tAATGGAATATATCGTAGGTCCGACTTTGTCTGAGTACATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATGTTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATagag                                                                                                             <  1:243118/141‑1 (MQ=255)
                            ggAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGatat                                                                                                         <  1:140691/141‑1 (MQ=255)
                             gAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATAtt                                                                                                        <  1:251967/141‑1 (MQ=255)
                               atatatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTaa                                                                                                      >  2:127670/1‑141 (MQ=255)
                                  tatCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGCTGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGcc                                                                                                   >  2:220204/1‑141 (MQ=255)
                                             cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGACTCAAATATTCGATGGCAGTAAACATCCGCGTGATCTGCGTATTGTCCAGCGAGATATTAGGCCCGAaaataaat                                                                                        >  2:309270/1‑141 (MQ=255)
                                             cGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAatatat                                                                                        <  1:226669/141‑1 (MQ=255)
                                               aCTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACCAAATATATTa                                                                                      >  2:220325/1‑141 (MQ=255)
                                                                tGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTAAATCAAAATTTTATTCCACAAAATATATTAATTGACAGCAATAAAAc                                                                     >  1:177982/1‑141 (MQ=255)
                                                                tGAAAGTCATGGGCAATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAAc                                                                     >  1:183616/1‑141 (MQ=255)
                                                                tGAAAGTCATGGGCAATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAAc                                                                     >  1:183615/1‑141 (MQ=255)
                                                                       cATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaa                                                              >  2:250583/1‑141 (MQ=255)
                                                                        aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGaaaa                                                             >  1:56963/1‑141 (MQ=255)
                                                                        aTGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGAACGCAATAAAACGTTGaaaa                                                             >  2:266653/1‑141 (MQ=255)
                                                                                     tgtgcacacAGGTATTAATTTTACGAATCAAATATTTGATGGCATTAAACACCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACACCAATAAAACGTTGAAAATATTTGATTTTgg                                                <  2:45859/136‑1 (MQ=255)
                                                                                         gACACAGCGATTAATGTTACGAATCAAATAGTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAGAATATATTAATTGACAGCAATAAAACGTTGAAAATGTTTGATTTTGGAAtt                                            >  1:156144/1‑141 (MQ=255)
                                                                                          acacAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTg                                           <  2:251703/141‑1 (MQ=255)
                                                                                               gCGATTAATTTTACGAAGCAAATATTGGATGGCATTAAACATCCGTATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTaaa                                      <  1:1561/141‑1 (MQ=255)
                                                                                                                acAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTc                     <  1:201781/140‑1 (MQ=255)
                                                                                                                 cAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCt                    >  2:93503/1‑141 (MQ=255)
                                                                                                                    atatTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTa                 <  1:270474/141‑1 (MQ=255)
                                                                                                                    atatTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTa                 >  1:146918/1‑141 (MQ=255)
                                                                                                                                   aaaCATCCTTATGATATGCGTAGTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt  <  2:138176/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAT  >  USA300TCH1516_ALE/1232328‑1232599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑