| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 1,103,408 | A→G | 10.8% | L310L (TTA→TTG) | pdhA → | Pyruvate dehydrogenase E1 component subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 1,103,408 | 0 | A | G | 10.8% | 102.7 / 5.8 | 37 | L310L (TTA→TTG) | pdhA | Pyruvate dehydrogenase E1 component subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (18/15); new base G (2/2); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ACTATGACATATCGTTATGGTCCTCATACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCAAAACTT > USA300TCH1516_ALE/1103271‑1103548 | aCTATGACATATCGTTATGGTCCTCATACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATgg > 1:27421/1‑141 (MQ=255)aCTATGACATATCGTTATGGTCCTCAGACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATgg < 1:100363/141‑1 (MQ=255) cTCATACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAg < 2:245759/141‑1 (MQ=255) tCATACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGt > 1:342075/1‑141 (MQ=255) tGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAAc > 1:268951/1‑141 (MQ=255) gCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGt > 2:325357/1‑141 (MQ=255) gTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTGTGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCaa > 2:36247/1‑141 (MQ=255) gTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTGTGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCaa > 2:36261/1‑141 (MQ=255) agACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAAAGGAAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCaaa < 2:347030/140‑1 (MQ=255) gACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCaaaa > 1:301312/1‑141 (MQ=255) aCGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAg < 1:226502/141‑1 (MQ=255) aCGATCCAACTCGTTACAGAACTACAGACCAAGATGCTGAATAGGAGAAAAAAGACCCATTAGTACGTTCCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAg < 1:208158/141‑1 (MQ=255) cGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGc > 2:161124/1‑141 (MQ=255) cGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGAACAGTTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGc > 1:291008/1‑141 (MQ=255) gATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCt > 1:184930/1‑141 (MQ=255) cGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATTGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTaaa < 2:55661/141‑1 (MQ=255) cGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTaaa < 2:27421/141‑1 (MQ=255) aGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAaga > 2:106875/1‑141 (MQ=255) aCGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAgg < 1:67504/141‑1 (MQ=255) cTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAaca > 1:160130/1‑141 (MQ=255) gggAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGaaa < 2:276766/141‑1 (MQ=255) gACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGtt > 2:246711/1‑141 (MQ=255) aCCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCAGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTa < 1:79118/141‑1 (MQ=255) aGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTGTGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTctct < 1:36261/141‑1 (MQ=255) aGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTGTGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTctct < 1:36247/141‑1 (MQ=255) aGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTctct < 1:111067/141‑1 (MQ=255) gTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTa < 1:325357/141‑1 (MQ=255) cGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATg > 1:209471/1‑141 (MQ=255) tttCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGa > 1:207742/1‑141 (MQ=255) gTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATta > 1:270517/1‑141 (MQ=255) aaaTTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATtatg < 2:342075/141‑1 (MQ=255) aaaTTCCGTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATtatg < 1:149169/141‑1 (MQ=255) cTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGaa > 2:10946/1‑141 (MQ=255) cTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGaa > 2:289634/1‑141 (MQ=255) cAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGcc < 1:53979/141‑1 (MQ=255) aaGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTc > 1:269250/1‑141 (MQ=255) aGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCa > 2:288046/1‑141 (MQ=255) aTGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCAAAACtt < 1:10946/141‑1 (MQ=255) | ACTATGACATATCGTTATGGTCCTCATACAATGGCTGGTGACGATCCAACTCGTTACAGAACTTCAGACGAAGATGCTGAATGGGAGAAAAAAGACCCATTAGTACGTTTCCGTAAATTCCTTGAAAACAAAGGTTTATGGAATGAAGACAAAGAAAATGAAGTTATTGAACGTGCAAAAGCTGATATTAAAGCAGCAATTAAAGAGGCTGATAACACTGAAAAACAAACTGTTACTTCTCTAATGGAAATTATGTATGAAGATATGCCTCAAAACTT > USA300TCH1516_ALE/1103271‑1103548 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |