BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	298069 =	115 (1.280)	4 (0.050)	4/252	14.4	3.6%	intergenic (+208/‑220)	tarK/tarF_1	Teichoic acid ribitol‑phosphate polymerase TarK/Teichoic acid glycerol‑phosphate transferase
*	?	USA300TCH1516_ALE	298125 =	110 (1.320)	4 (0.050)	4/252	14.4	3.6%	intergenic (+264/‑164)	tarK/tarF_1	Teichoic acid ribitol‑phosphate polymerase TarK/Teichoic acid glycerol‑phosphate transferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

TGAAGTGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGTATTTTAT  >  USA300TCH1516_ALE/297929‑298205

tGAAGTGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCa                                                                                                                                          >  1:450043/1‑141 (MQ=255)

    gTGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCg                                                                                                                                      <  2:129611/141‑1 (MQ=255)

     tGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGt                                                                                                                                     >  3:7409/1‑141 (MQ=255)

     tGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGt                                                                                                                                     >  1:375173/1‑141 (MQ=255)

     tGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGt                                                                                                                                     <  1:573743/141‑1 (MQ=255)

      gAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGtt                                                                                                                                    <  2:429446/141‑1 (MQ=255)

           aCATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTa                                                                                                                               >  1:97982/1‑141 (MQ=255)

           aCATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTa                                                                                                                               >  4:259807/1‑141 (MQ=255)

            cATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTaa                                                                                                                              <  1:545255/141‑1 (MQ=255)

              tGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAAtt                                                                                                                            <  1:71475/141‑1 (MQ=255)

                    gatgaGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATaaa                                                                                                                      <  2:375173/141‑1 (MQ=255)

                       gagaAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGGTATCTAATTCATAAAtgt                                                                                                                   >  2:245360/1‑141 (MQ=255)

                               ggACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAacac                                                                                                           >  2:644102/1‑141 (MQ=255)

                                  cTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATa                                                                                                        <  2:382877/141‑1 (MQ=255)

                                    ataCAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACg                                                                                                      >  1:285998/1‑141 (MQ=255)

                                    ataCAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACg                                                                                                      >  1:152833/1‑141 (MQ=255)

                                     taCAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGt                                                                                                     >  2:505539/1‑141 (MQ=255)

                                     taCAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCAACTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGt                                                                                                     <  1:421957/141‑1 (MQ=255)

                                       cAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTa                                                                                                   >  2:134077/1‑141 (MQ=255)

                                         aTGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGGAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATg                                                                                                 <  4:39147/141‑1 (MQ=255)

                                         aTGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAAACAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATg                                                                                                 <  3:2733/141‑1 (MQ=255)

                                           gagaATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGaa                                                                                               <  1:65169/141‑1 (MQ=255)

                                            agaATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGaaa                                                                                              >  1:244827/1‑140 (MQ=255)

                                              aaTAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATgg                                                                                            >  3:120071/1‑141 (MQ=255)

                                                 aaCCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATGGTCATTTGGGAAAAAAAAATAATCGATCCAAGCGTTATCTAATTCATAAATGTGTAAACACAAAAGTTATGAATGGATa                                                                                         >  1:456994/1‑141 (MQ=255)

                                                  aCCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATaa                                                                                        >  1:274464/1‑141 (MQ=255)

                                                   ccTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATaaa                                                                                       >  4:88126/1‑140 (MQ=255)

                                                     ttttGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGa                                                                                     <  1:162649/141‑1 (MQ=255)

                                                       ttGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGAtt                                                                                   >  1:310268/1‑141 (MQ=255)

                                                       ttGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGAtt                                                                                   <  2:152833/141‑1 (MQ=255)

                                                            ttttCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtg                                                                              >  2:146075/1‑141 (MQ=255)

                                                             tttCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgt                                                                             >  2:17107/1‑141 (MQ=255)

                                                             tttCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgt                                                                             >  2:501245/1‑141 (MQ=255)

                                                              ttCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgtt                                                                            >  3:267867/1‑141 (MQ=255)

                                                              ttCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgtt                                                                            >  2:114567/1‑141 (MQ=255)

                                                               tcattcatAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgtta                                                                           >  4:121920/1‑141 (MQ=255)

                                                               tCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgtta                                                                           <  3:84614/141‑1 (MQ=255)

                                                               tCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATATGTAAACACATACGTTATGAATGCATAACGCTTTTTtgtta                                                                           >  1:403041/1‑141 (MQ=255)

                                                                 aTGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatg                                                                         <  1:110043/141‑1 (MQ=255)

                                                                 aTGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatg                                                                         <  1:151425/141‑1 (MQ=255)

                                                                  tGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatgt                                                                        <  4:250531/141‑1 (MQ=255)

                                                                   gcgtAAGGCGATTCAATCAAAAGCGATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAAGCGATCCAATCGTTATCTAATTCATAAATGTGGAAACACAGACGTTATGAATGGATAACGATGTGTtgttatgtt                                                                       >  4:309/4‑141 (MQ=255)

                                                                   gCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatgtt                                                                       >  4:198536/1‑141 (MQ=255)

                                                                   gCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatgtt                                                                       >  2:407073/1‑141 (MQ=255)

                                                                   gCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTtgttatgtt                                                                       <  2:545472/141‑1 (MQ=255)

                                                                     aTAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTATAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTaa                                                                     <  3:87372/141‑1 (MQ=255)

                                                                     aTAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTaa                                                                     <  1:30695/141‑1 (MQ=255)

                                                                      tAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTaaa                                                                    <  1:361144/141‑1 (MQ=255)

                                                                       aaGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAg                                                                   <  3:130982/141‑1 (MQ=255)

                                                                           cGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGt                                                               <  3:262088/141‑1 (MQ=255)

                                                                              ttCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACa                                                            >  2:209801/1‑141 (MQ=255)

                                                                               aaaatcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACAt                                                           <  2:385417/139‑1 (MQ=255)

                                                                                caatcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACAtt                                                          >  3:195956/1‑141 (MQ=255)

                                                                                caatcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGAGAACGATTTGTTGTTATGTTAAAGTGGTACAtt                                                          >  4:73951/1‑141 (MQ=255)

                                                                                  atcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATa                                                                                                        >  1:487330/1‑93 (MQ=255)

                                                                                  atcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGCTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTaa                                                        >  1:676849/1‑141 (MQ=255)

                                                                                   tcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAAt                                                       >  1:387072/1‑141 (MQ=255)

                                                                                   tcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAAt                                                       >  2:275151/1‑141 (MQ=255)

                                                                                   tcaaAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAAt                                                       >  3:104124/1‑141 (MQ=255)

                                                                                       aaGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTAAATTAATAATg                                                   >  1:225949/1‑141 (MQ=255)

                                                                                          cAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAt                                                >  2:678278/1‑141 (MQ=255)

                                                                                           aaTCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAtt                                               >  1:308150/1‑141 (MQ=255)

                                                                                           aaTCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAtt                                               >  1:361080/1‑141 (MQ=255)

                                                                                           aaTCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAtt                                               >  2:573029/1‑141 (MQ=255)

                                                                                            aTCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAttt                                              >  2:627228/1‑141 (MQ=255)

                                                                                            aTCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTAttt                                              >  1:44206/1‑141 (MQ=255)

                                                                                             tCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTc                                             <  3:177776/141‑1 (MQ=255)

                                                                                               aCCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGt                                           >  1:125871/1‑141 (MQ=255)

                                                                                                 cTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTAt                                         <  1:46533/141‑1 (MQ=255)

                                                                                                 cTTCCAACTGAATTGTCAATTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTAt                                         <  3:2611/141‑1 (MQ=255)

                                                                                                  ttCCAATTGAATTGTCATTTTGTAAAATAAAATAATCGCTCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATg                                        <  2:582639/141‑1 (MQ=255)

                                                                                                  ttCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCTTGTATTTCGTATg                                        <  2:591900/141‑1 (MQ=255)

                                                                                                  ttCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATg                                        <  4:160648/141‑1 (MQ=255)

                                                                                                    ccAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGAt                                      >  1:378862/1‑141 (MQ=255)

                                                                                                    ccAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGAt                                      <  2:154568/141‑1 (MQ=255)

                                                                                                       aCTGAATTGTCATTTTTTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAAt                                   <  3:170362/141‑1 (MQ=255)

                                                                                                       aCTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAAt                                   <  2:511047/141‑1 (MQ=255)

                                                                                                       aCTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAAt                                   >  3:267880/1‑141 (MQ=255)

                                                                                                       aCTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAAt                                   <  2:446525/141‑1 (MQ=255)

                                                                                                          gAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTaa                                <  1:505539/141‑1 (MQ=255)

                                                                                                             ttGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGa                             <  4:143292/141‑1 (MQ=255)

                                                                                                             ttGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGa                             <  1:77491/141‑1 (MQ=255)

                                                                                                                tCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTTTTATGTTAAATTGGTACATTAATCATGTATTTCGTATGATAATTAACGACaa                          >  4:88404/1‑141 (MQ=255)

                                                                                                                tCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACaa                          <  1:146075/141‑1 (MQ=255)

                                                                                                                 cATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAg                         <  1:581188/141‑1 (MQ=255)

                                                                                                                    tttGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAgtgt                      >  1:43481/1‑141 (MQ=255)

                                                                                                                     ttGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTa                     <  4:126355/141‑1 (MQ=255)

                                                                                                                      tGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTaa                    <  1:134077/141‑1 (MQ=255)

                                                                                                                         aaaataaaatAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTa                                 >  4:20853/1‑125 (MQ=255)

                                                                                                                         aaaataaaatAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTa                                 <  3:20853/125‑1 (MQ=255)

                                                                                                                          aaataaaataATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGt                <  4:195956/141‑1 (MQ=255)

                                                                                                                            ttaaaataaTCGTTCCATTCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTTCATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTa              <  1:577891/140‑1 (MQ=255)

                                                                                                                            ataaaataaTCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTa              >  4:234265/1‑141 (MQ=255)

                                                                                                                            ataaaataaTCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTa              <  2:308150/141‑1 (MQ=255)

                                                                                                                                 ataatCGATCCAATCGTTATTTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATGATGTATTTCATATGATAATTAACGACAAGTGTAATGGTTAAATGt         >  4:226411/1‑141 (MQ=255)

                                                                                                                                 ataatCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGt         >  4:39823/1‑141 (MQ=255)

                                                                                                                                   aatCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGTAt       <  3:206835/141‑1 (MQ=255)

                                                                                                                                      cGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAANGTAtttt    >  2:526739/1‑141 (MQ=255)

                                                                                                                                       gATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGTATTTTa   >  3:250143/1‑141 (MQ=255)

                                                                                                                                        aTCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGTATTTTat  >  3:188239/1‑141 (MQ=255)

TGAAGTGAGCAACATGTCATGATGAGAAGCAGGACTATACAATGAGAATAACCTTTTGATTTTTCATGCATAAGGCGATTCAATCAAAAGCAATCACCTTCCAACTGAATTGTCATTTTGTAAAATAAAATAATCGATCCAATCGTTATCTAATTCATAAATGTGTAAACACATACGTTATGAATGGATAACGATTTTTTGTTATGTTAAAGTGGTACATTAATCATGTATTTCGTATGATAATTAACGACAAGTGTAATGGTTAAATGTATTTTAT  >  USA300TCH1516_ALE/297929‑298205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.