Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 1,064,016 G→A A158V (GCA→GTA)  ywtF ← Putative transcriptional regulator YwtF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,064,0160GA97.5% 276.2 / ‑2.8 81A158V (GCA→GTA) ywtFPutative transcriptional regulator YwtF
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (39/39);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

AAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAT  >  USA300TCH1516_ALE/1063882‑1064146
                                                                                                                                      |                                                                                                                                  
aaGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCa                                                                                                                              <  1:388081/141‑1 (MQ=255)
 aGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCat                                                                                                                             >  4:95296/1‑141 (MQ=255)
  gCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCata                                                                                                                            >  2:223886/1‑141 (MQ=255)
     tcatcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTg                                                                                                                         <  1:424210/141‑1 (MQ=255)
      catcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCATGCTCATTTCCAGCCTCGACAAATACTTTCATATTGa                                                                                                                        >  2:411278/1‑141 (MQ=255)
       atcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGAc                                                                                                                       <  2:439326/141‑1 (MQ=255)
        tcaCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGAct                                                                                                                      >  1:461261/1‑141 (MQ=255)
        tcaCCATTTAATTTTTGGTACCCTTTGTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGTct                                                                                                                      <  2:112318/141‑1 (MQ=255)
         caCCATTTAATTTTTGGTACCCTTTTTTTATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGActc                                                                                                                     >  2:385646/1‑141 (MQ=255)
          aCCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGActct                                                                                                                    <  2:608591/141‑1 (MQ=255)
             aTTTAATTTTTGTTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTcac                                                                                                                 <  1:303536/141‑1 (MQ=255)
             aTTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTcac                                                                                                                 <  4:80603/141‑1 (MQ=255)
                tAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACata                                                                                                              >  1:165537/1‑141 (MQ=255)
                tAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACata                                                                                                              >  1:165719/1‑141 (MQ=255)
                 aaTTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             <  1:79123/141‑1 (MQ=255)
                   tttttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataata                                                                                                           <  1:417242/141‑1 (MQ=255)
                   tttttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataata                                                                                                           >  4:241648/1‑141 (MQ=255)
                      ttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATc                                                                                                        <  1:429324/141‑1 (MQ=255)
                       tGGTACCCTTTTTTTTTTTTTTTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATTCCACCAAGCTCATTTACAGCCTCGACAAATTCTTTCATATTTACTCTCACATAATAATCa                                                                                                       >  2:471387/1‑141 (MQ=255)
                       tGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCa                                                                                                       >  1:162135/1‑141 (MQ=255)
                           aCCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAg                                                                                                   >  3:196389/1‑141 (MQ=255)
                            cccTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTTACTCTCACATAATAATCAACAgg                                                                                                  >  4:298257/1‑141 (MQ=255)
                             ccTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGt                                                                                                 <  1:32529/141‑1 (MQ=255)
                              cTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa                                                                                                <  4:113963/141‑1 (MQ=255)
                                 ttttaattttaaTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACAt                                                                                             <  1:371637/141‑1 (MQ=255)
                                 ttttaattttaaTCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACAt                                                                                             >  1:239069/1‑141 (MQ=255)
                                    taattttaatCTTACCTGTATTATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCa                                                                                          <  2:552053/141‑1 (MQ=255)
                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             <  1:209630/119‑1 (MQ=255)
                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataa                                                                                                             >  2:209630/1‑119 (MQ=255)
                                       ttttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATgg                                                                                       >  1:371582/1‑141 (MQ=255)
                                        tttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGt                                                                                      >  3:82931/1‑141 (MQ=255)
                                         ttaatCTTACCAGTATCATATGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCGCACATAATAATCAACAGGTACATTCAttctg                                                                                     <  2:653870/141‑5 (MQ=255)
                                            atCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCt                                                                                  <  2:496634/141‑1 (MQ=255)
                                               ttACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCt                                                                               >  4:105447/1‑141 (MQ=255)
                                                tACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCtc                                                                              >  3:256518/1‑140 (MQ=255)
                                                gaCCAGTATCATCTGGGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGTGACATTCATGGTAGCTTTTa                                                                              >  4:138601/2‑141 (MQ=255)
                                                 aCCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTAc                                                                             <  1:446358/141‑1 (MQ=255)
                                                   cAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCg                                                                           <  1:337161/141‑1 (MQ=255)
                                                       atcatcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATc                                                                       >  1:172001/1‑141 (MQ=255)
                                                        tcatcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATcc                                                                      >  1:231517/1‑141 (MQ=255)
                                                           tcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCAtt                                                                   <  3:124668/141‑1 (MQ=255)
                                                               tgtTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAGCAGGTACATTCATGCTAGCTTCTACCGAATCCCTTGCgg                                                               <  2:257961/141‑1 (MQ=255)
                                                                 ttAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCa                                                             >  1:395342/1‑141 (MQ=255)
                                                                   aGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATGATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAAt                                                           <  2:364934/141‑1 (MQ=255)
                                                                     gTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTg                                                         <  2:461261/141‑1 (MQ=255)
                                                                      ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg                                                        >  4:178748/1‑141 (MQ=255)
                                                                      ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg                                                        <  3:83018/141‑1 (MQ=255)
                                                                      ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACAAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg                                                        <  1:536469/141‑1 (MQ=255)
                                                                        cATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGac                                                      <  2:657222/141‑1 (MQ=255)
                                                                         aTTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGacc                                                     <  3:230826/141‑1 (MQ=255)
                                                                          tttAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGacca                                                    >  2:264369/1‑141 (MQ=255)
                                                                              aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGAccacca                                                 >  3:88606/1‑140 (MQ=255)
                                                                              aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGAccacca                                                 <  4:88606/140‑1 (MQ=255)
                                                                              aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCat                                                >  2:259803/1‑141 (MQ=255)
                                                                                 cGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtg                                             <  1:467560/141‑1 (MQ=255)
                                                                                  gTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgc                                            >  3:93044/1‑141 (MQ=255)
                                                                                   tATGGCACATCATATTATATGCCACCAAGCTCATTTACAGCCACGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca                                           <  2:264481/141‑1 (MQ=255)
                                                                                   tATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca                                           >  4:218957/1‑141 (MQ=255)
                                                                                   tATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca                                           <  3:95296/141‑1 (MQ=255)
                                                                                         acaTCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGAGCCACCAGAtgcatgtgca                                     >  1:393161/1‑141 (MQ=255)
                                                                                           atcatAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATGTTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgcatgtgcatg                                   >  3:234093/1‑141 (MQ=255)
                                                                                              atAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGt                                <  1:674605/141‑1 (MQ=255)
                                                                                                 gTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAAt                             >  2:367712/1‑141 (MQ=255)
                                                                                                      tGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTAt                        >  2:394156/1‑141 (MQ=255)
                                                                                                        ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa                                                                                                >  3:140089/1‑67 (MQ=255)
                                                                                                        ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa                                                                                                <  4:140089/67‑1 (MQ=255)
                                                                                                        ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCg                      >  4:170196/1‑141 (MQ=255)
                                                                                                        ccaccaAGCTCATTTACAGCCTAGACAAATAATTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCTATTGCGGCAATTGGACTACCATATGCATGTGCATGGGTAATATTATCg                      >  4:44294/1‑141 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGa                                                       >  1:306211/1‑102 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGa                                                       <  2:306211/102‑1 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                <  3:38912/141‑1 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                <  2:269341/141‑1 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                >  4:166811/1‑141 (MQ=255)
                                                                                                              aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg                <  2:64297/141‑1 (MQ=255)
                                                                                                               gCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGc               >  4:7254/1‑141 (MQ=255)
                                                                                                                cTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGcc              >  1:200224/1‑141 (MQ=255)
                                                                                                                cTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGcc              <  2:606962/141‑1 (MQ=255)
                                                                                                                        cAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAg      <  2:656617/141‑1 (MQ=255)
                                                                                                                         aGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAgg     <  3:281601/141‑1 (MQ=255)
                                                                                                                            cTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAt  >  1:617355/1‑141 (MQ=255)
                                                                                                                            cTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAt  >  1:446235/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AAGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGGAAT  >  USA300TCH1516_ALE/1063882‑1064146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑