Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 39,246 C→T A630T (GCT→ACT)  pbp ← Beta‑lactam‑inducible penicillin‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE39,2460CT100.0% 325.2 / NA 93A630T (GCT→ACT) pbpBeta‑lactam‑inducible penicillin‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/45);  total (48/45)

ATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  USA300TCH1516_ALE/39110‑39382
                                                                                                                                        |                                                                                                                                        
aTTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTcatc                                                                                                                                      >  2:73928/1‑141 (MQ=255)
  tGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTcatcat                                                                                                                                    <  1:635006/141‑1 (MQ=255)
   gCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTcatcatc                                                                                                                                   <  2:410582/141‑1 (MQ=255)
        aCTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGtt                                                                                                                              <  1:415873/141‑1 (MQ=255)
        aCTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGtt                                                                                                                              <  2:487942/141‑1 (MQ=255)
         cTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGttt                                                                                                                             >  3:226509/1‑141 (MQ=255)
           gTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTgg                                                                                                                           <  1:674972/141‑1 (MQ=255)
             tttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAt                                                                                                                         <  1:600452/141‑1 (MQ=255)
             tttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAt                                                                                                                         <  4:88091/141‑1 (MQ=255)
             tttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAt                                                                                                                         >  1:286268/1‑141 (MQ=255)
              ttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAtt                                                                                                                        <  1:18119/141‑1 (MQ=255)
                gTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTAt                                                                                                                      <  4:90360/141‑1 (MQ=255)
                      cATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTAt                                                                                                                <  1:599569/141‑1 (MQ=255)
                            tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             <  3:182872/138‑1 (MQ=255)
                            tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             >  4:182872/1‑138 (MQ=255)
                              tCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGat                                                                                                        >  2:125734/1‑141 (MQ=255)
                                gTATTTTTTTTTTTTTTTTTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTTATATTCTTCTTCTTGTTTGGATTATCTTTATCATATGatat                                                                                                      >  2:215470/1‑141 (MQ=255)
                                   ttttttATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATNATATaaa                                                                                                   >  2:526983/1‑141 (MQ=255)
                                         attaCCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCAccc                                                                                             <  1:646962/141‑1 (MQ=255)
                                         attaCCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCAccc                                                                                             <  1:101350/141‑1 (MQ=255)
                                           taCCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCaa                                                                                           <  2:436866/141‑1 (MQ=255)
                                             ccGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAAtt                                                                                         <  1:660853/141‑1 (MQ=255)
                                                ttCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGt                                                                                      >  2:375372/1‑141 (MQ=255)
                                                  ctcATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCt                                                                                    >  4:188479/1‑141 (MQ=255)
                                                    cATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGc                                                                                  >  2:358948/1‑141 (MQ=255)
                                                      tataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCa                                                                                >  4:155439/1‑141 (MQ=255)
                                                       ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  4:88870/141‑1 (MQ=255)
                                                       ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  4:274698/141‑1 (MQ=255)
                                                         aGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGtt                                                                             <  2:477229/141‑1 (MQ=255)
                                                             catcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTtctc                                                                         >  4:243375/1‑141 (MQ=255)
                                                              atcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCATTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTcc                                                                        >  1:529109/1‑141 (MQ=255)
                                                               tcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATACGTTTATCTTGTCCATATTTAACATCAAACGTCTTCTTTATCGTTGTATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCt                                                                       <  3:258186/141‑1 (MQ=255)
                                                                 atACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTg                                                                     <  1:147897/141‑1 (MQ=255)
                                                                   acacTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTACTTGtt                                                                   >  2:621259/1‑141 (MQ=255)
                                                                      cTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAaccac                                                                                               <  2:372844/110‑1 (MQ=255)
                                                                      cTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAaccac                                                                                               >  1:372844/1‑110 (MQ=255)
                                                                      cTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCa                                                                >  1:563536/1‑141 (MQ=255)
                                                                       tttACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAGTTTGTCTGCCAGTTTCTCCTTGTTTCAt                                                               >  2:511530/1‑141 (MQ=255)
                                                                       tttACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAt                                                               >  1:235704/1‑141 (MQ=255)
                                                                       tttACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAt                                                               >  4:266099/1‑141 (MQ=255)
                                                                         tACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAttt                                                             <  1:73928/141‑1 (MQ=255)
                                                                          aCCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATACACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAtttt                                                            <  2:386706/141‑1 (MQ=255)
                                                                           ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTg                                                           <  2:435465/141‑1 (MQ=255)
                                                                           ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTg                                                           >  2:700448/1‑141 (MQ=255)
                                                                           ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTg                                                           >  2:248171/1‑141 (MQ=255)
                                                                           ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCATTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTGGTCTGCCAGTTTTTCCTTGTTTCAttttt                                                           >  4:209705/1‑140 (MQ=255)
                                                                               agaTTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGtt                                                       >  3:129287/1‑141 (MQ=255)
                                                                                gaTTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTc                                                      <  1:608284/141‑1 (MQ=255)
                                                                                     tGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAg                                                 <  1:417777/141‑1 (MQ=255)
                                                                                      ggCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGt                                                <  2:226307/141‑1 (MQ=255)
                                                                                      ggCATTGTAGCTAGCCATTCCTTTATCTTATACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGt                                                <  1:696708/141‑1 (MQ=255)
                                                                                       gCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTa                                               >  1:499525/1‑141 (MQ=255)
                                                                                       gCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTa                                               >  1:499440/1‑141 (MQ=255)
                                                                                       gCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACAACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTa                                               >  1:581004/1‑141 (MQ=255)
                                                                                        cATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGATTTCTGCAGTAc                                              >  2:177830/1‑141 (MQ=255)
                                                                                        cATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTAc                                              <  2:378575/141‑1 (MQ=255)
                                                                                        cATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTAc                                              >  1:261621/1‑141 (MQ=255)
                                                                                               gctagcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGAttt                                       >  1:147818/1‑141 (MQ=255)
                                                                                                ctagcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTg                                      >  2:517916/1‑141 (MQ=255)
                                                                                                 tagcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGc                                     <  1:11183/141‑1 (MQ=255)
                                                                                                 tagcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGc                                     >  2:380467/1‑141 (MQ=255)
                                                                                                  agcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGcc                                    <  3:40465/141‑1 (MQ=255)
                                                                                                   gcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCa                                   >  1:382808/1‑141 (MQ=255)
                                                                                                     cATTCCTTTATCTTGTACATCTTTAACATTAATAGTcatcatc                                                                                                                                   >  2:234039/1‑43 (MQ=255)
                                                                                                     cATTCCTTTATCTTGTACATCTTTAACATTAATAGTcatcatc                                                                                                                                   <  1:234039/43‑1 (MQ=255)
                                                                                                     cATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTAc                                              >  2:517157/1‑128 (MQ=255)
                                                                                                     cATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTAc                                              <  1:517157/128‑1 (MQ=255)
                                                                                                     cATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAAt                                 <  2:639312/141‑1 (MQ=255)
                                                                                                       ttCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTa                               <  4:160743/141‑1 (MQ=255)
                                                                                                          cTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTATCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGt                            <  2:652157/141‑1 (MQ=255)
                                                                                                           tttATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCAAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGtt                           >  2:678824/1‑141 (MQ=255)
                                                                                                                cTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCCGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCAt                      >  2:372707/1‑141 (MQ=255)
                                                                                                                 ttGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATa                     <  1:334868/141‑1 (MQ=255)
                                                                                                                  tGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATaa                    <  2:630982/141‑1 (MQ=255)
                                                                                                                   gTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAg                   <  2:115991/141‑1 (MQ=255)
                                                                                                                   gTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAg                   >  3:207295/1‑141 (MQ=255)
                                                                                                                   gTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAg                   >  1:584072/1‑141 (MQ=255)
                                                                                                                   gTACATCTTTAACATTAATAGTCAGCATCATGGTTGGGTTATCTTTATCATATGATATTAACCACTCAATTTGTATGTTAGTTTCACCGTGTTTCATCTTTAGGTCTGCATGATCGGAATTGCCATTTAAGTTTTCAttaa                   >  4:49247/1‑138 (MQ=255)
                                                                                                                      cATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATc                >  2:629179/1‑141 (MQ=255)
                                                                                                                        tCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCta              <  2:399397/141‑1 (MQ=255)
                                                                                                                           ttAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATaa           <  2:422947/141‑1 (MQ=255)
                                                                                                                           ttAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACACAATTTGTCTACCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGATTGCATAAGATCTATaa           >  1:368588/1‑141 (MQ=255)
                                                                                                                             aaCATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAat         <  1:261784/141‑1 (MQ=255)
                                                                                                                                aTTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtc      <  1:125734/141‑1 (MQ=255)
                                                                                                                                aTTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtc      >  1:687316/1‑141 (MQ=255)
                                                                                                                                 ttAATAGTCATCATCATGATTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTAATCCATGTTTAATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtct     <  1:177830/141‑1 (MQ=255)
                                                                                                                                   aaTAGTCATCATCATGTTTGGATTATCTTTATCATATGGTATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttc   >  1:373064/1‑141 (MQ=255)
                                                                                                                                   aaTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCATTTTCGCTTTGTTTCATAATAAATGGTGCAGTAACGGATTTGCCAATGAAGTTTGCATAAGATCTATAAATAtcttt   >  1:245698/1‑140 (MQ=255)
                                                                                                                                   aaTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttc   >  3:136093/1‑141 (MQ=255)
                                                                                                                                    aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       >  3:305065/1‑136 (MQ=255)
                                                                                                                                    aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGATTCATTTTAAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttct  >  3:254612/1‑141 (MQ=255)
                                                                                                                                    aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCAACTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttct  >  1:602984/1‑141 (MQ=255)
                                                                                                                                    aTAGTCATCATCATGATTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       <  4:305065/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  USA300TCH1516_ALE/39110‑39382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑